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Zwilch (NM_026507) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216108
Zwilch (tGFP-tagged) - Mouse Zwilch kinetochore associated homolog (Drosophila) (Zwilch), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $810.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Zwilch or NM_026507"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Zwilch
Synonyms 2310031L18Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216108 representing NM_026507
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGGAGGCCACGGCGGTATGTGGTCGCGAATGAACCGCGCGGCGGAGGAGTTTTATGCTCGTCTCC
GGCAGGAATTTAATGAAGAGAAGAAAGGAGCCTCTAAAGACCCATTCATCTATGAGGCTGATGTACAAGT
GCAGTTGATCAGCAAAGGCCAACCAAGTCTTCTGAAAACTATTCTAAATGAAAATGACTCAGTGTTTCTC
GTGGAAAAAGTGGTTTTGGAGAAGGAAGAAACAAGTCAAGTTGAAGAACTGCAGTCTGAAGAAACTGCTA
TTTCTGATCTCTCTGCTGGTGAAAATATTAGGCCACTGGCCTTACCTGTTGGGAGAGCAAGGCAGTTAAT
TGGGCTCTATACCATGGCTCACAATCCTAATATGACTCATCTGAAGATTAAGCAGCCAGTGACAGCCCTT
CCTCCTCTTTGGGTGAGATGTGACGGTTCAGATCCTGAAGGTACCTGTTGGCTAGGAGCTGAGCTTATCA
CAACAAATGACATCATTGCAGGCGTTATCCTGTATGTGCTCACTTGTAAAGCTGATAAAAATTACTCTGA
AGATCTGGAAAATTTAAAAACTTCACACAAGAAAAGACATCACGTGTCTGCTGTGACAGCCAGAGGCTTT
GCCCAGTATGAGCTCTTTAAGTCTGACGACTTGGATGACACTGTTGCCCCATCACAAACTACAGTCACGC
TGGACCTTTCCTGGAGCCCTGTGGATGAGATGCTCCAAACCCCTCCCCTGTCTTCAACTGCAGCTCTGAA
TATCAGAGTACAATCAGGAGAGTCCAGAGGTTGTCTGAGTCATCTTCACAGAGAACTGAAATTTCTCCTT
GTTTTAGCTGATGGCATCAGGACTGGTGTAACTGAATGGCTTGAGCCTCTGGAAACAAAATCTGCTCTTG
AATTTGTTCAGGAATTTCTGAATGACTTAAATAAACTGGATGAATTTGATGATTCTACAAAAAAAGATAA
ACAAAAAGAGGCAGTGAACCATGACGCAGCTGCAGTTGTCAGGTCCATGCTTCTCACCGTGCGGGGGGAT
CTCGATTTTGCTGAACAGCTTTGGTGTAGAATGAGCAGCAGTGTGGTTTCCTATCAAGACTTGGTGAAGT
GTTTCACACTCATCCTGCAGAGTCTTCAGCGTGGTGATATACAGCCATGGTTGCACAGTGGAAGTAACAG
TTTACTAAGTAAACTCATTCATCAGTCTTACCATGGAGCTATGGACAGTGTCCCTCTCTCTGGAACTACC
CCACTGCAGATGCTTTTGGAAATTGGTTTGGACAAACTGAAGAAAGATTATATCAGCTTTTTTGTCAGTC
AGGAACTTGCATCTTTGAATCATTTGGAATACTTCATTTCTCCCTCAGTATCGACACAGGAACAGGTTTG
CCGTGTTCAGAAACTCCATCATATTCTTGAAATCTTAGTCATCTGCATGCTTTTCATTAAACCTCAGCAT
GAGCTTCTCTTCTCTTTAACACAATCCTGCATAAAATATTATAAACAAAATCCTCTTGATGAGCAACACA
TTTTTCAGTTGCCAGTCAGACCAGCTGCTGTCAAGAATTTGTATCAAAGTGAGAAGCCGCAGAAATGGAG
AGTGGAGCTCAGCAATAGTCAGAAGAGGGTGAAAACAGTGTGGCAACTGAGTGACAGCTCCCCTGTAGAC
CATTCCAGTTTCCACAGGCCTGAGTTTCCTGAGCTGACACTGAATGGTAGTCTGGAAGAAAGGACAGCCT
TCGTTAACATGCTTACCTGCAGCCAGGTGCACTTCAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216108 representing NM_026507
Red=Cloning site Green=Tags(s)

MDGGHGGMWSRMNRAAEEFYARLRQEFNEEKKGASKDPFIYEADVQVQLISKGQPSLLKTILNENDSVFL
VEKVVLEKEETSQVEELQSEETAISDLSAGENIRPLALPVGRARQLIGLYTMAHNPNMTHLKIKQPVTAL
PPLWVRCDGSDPEGTCWLGAELITTNDIIAGVILYVLTCKADKNYSEDLENLKTSHKKRHHVSAVTARGF
AQYELFKSDDLDDTVAPSQTTVTLDLSWSPVDEMLQTPPLSSTAALNIRVQSGESRGCLSHLHRELKFLL
VLADGIRTGVTEWLEPLETKSALEFVQEFLNDLNKLDEFDDSTKKDKQKEAVNHDAAAVVRSMLLTVRGD
LDFAEQLWCRMSSSVVSYQDLVKCFTLILQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGAMDSVPLSGTT
PLQMLLEIGLDKLKKDYISFFVSQELASLNHLEYFISPSVSTQEQVCRVQKLHHILEILVICMLFIKPQH
ELLFSLTQSCIKYYKQNPLDEQHIFQLPVRPAAVKNLYQSEKPQKWRVELSNSQKRVKTVWQLSDSSPVD
HSSFHRPEFPELTLNGSLEERTAFVNMLTCSQVHFK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026507
ORF Size 1788 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026507.4, NP_080783.3
RefSeq Size 2784 bp
RefSeq ORF 1791 bp
Locus ID 68014
UniProt ID Q8R060
Cytogenetics 9 C
Summary Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026507" in other vectors (4)
SKU Description Size Price
MC204160 Zwilch (untagged) - Mouse Zwilch, kinetochore associated, homolog (Drosophila) (Zwilch), (10ug) 10 ug
$549.00
MR216108 Zwilch (Myc-DDK-tagged) - Mouse Zwilch, kinetochore associated, homolog (Drosophila) (Zwilch) 10 ug
$610.00
MR216108L3 Lenti ORF clone of Zwilch (Myc-DDK-tagged) - Mouse Zwilch, kinetochore associated, homolog (Drosophila) (Zwilch) 10 ug
$910.00
MR216108L4 Lenti ORF clone of Zwilch (mGFP-tagged) - Mouse Zwilch, kinetochore associated, homolog (Drosophila) (Zwilch) 10 ug
$910.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.