OriGene Logo

Mgat4a (NM_173870) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MG216106
Mgat4a (tGFP-tagged) - Mouse mannoside acetylglucosaminyltransferase 4 isoenzyme A (Mgat4a), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $698.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8
100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "Mgat4a or NM_173870"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mgat4a
Synonyms 9530018I07Rik; glcNAc-T-IVa; GnT-IVa
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216106 representing NM_173870
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCTCCGAAATGGAACTGTGGCCACTGCGCTGGTATTTGTCACGTCCTTCCTTACCCTATCCTGGT
ATACCACGTGGCAAAATGGGAAAGAAAAACTAATTGCTTATCAACGAGAATTCCTTGCTCTAAAAGAGCG
TCTTCGAGTGGCCGAGCATAGGATATCTCAGCGCTCCTCGGAGCTAAACACCATTGTCCAGCAGTTCCGC
AGAGCTGGAGCAGAGACTAATGGAAGTAAGACAGCTCTGAGTACAATCTCAGATAATACCATAAAGCTTC
TAAAAGAGTTGACAAGCAAAAAATCACTTCGAGTGCCAAGTATTTATTATCATTTGCCTCATCTATTGCA
AAATGAAAGAAGCCTTCAGCCCGCCGTACAGATTGGCAGTGGAAGAACGGGAGTTTCAATAGTTATGGGA
ATTCCTACTGTGAAGAGAGAAGTTAAATCTTACCTCGTAGAAACCCTTCACTCCCTTATTGATAATCTGT
ATCCTGAAGAGAAGCTGGACTGTGTTATAGTCGTCTTCATAGGAGAGACAGATCTTGATTATGTTCACAG
CGTTGTTGCCAACCTGGAGAAAGAATTTTCTAGAGAAATTAGTTCTGGCCTGCTGGAAATAATCTCTCCT
CCTGAAAGCTATTACCCCGACTTGACAAACCTGAAGGAGACGTTCGGAGACTCCAAGGAAAGAGTGAGAT
GGAGAACCAAGCAAAACCTGGATTACTGTTTTCTGATGATGTATGCTCAGGAGAAGGGCATCTACTACAT
TCAGCTTGAAGACGATATTATTGTCAAACAAAACTATTTTAATACCATAAAGAATTTTGCACTTCAACTT
TCTTCGGAAGAATGGATGATTCTAGAGTTTTCCCAGCTTGGCTTCATTGGAAAAATGTTCCAGGCGCCGG
ACCTGGCGCTGGTCGTGGAGTTCATCCTCATGTTCTATAAGGAGAAGCCCATTGACTGGCTGCTGGACCA
CATTCTCTGGGTGAAGGTCTGCAACCCCGAAAAAGATGCTAAACACTGCGACAGACAGAAGGCAAACCTA
CGAATCCGCTTCCGACCCTCCCTCTTCCAGCACGTGGGCCTACACTCGTCTCTGTCGGGGAAGATTCAGA
AACTCACGGATAAAGATTACATGAAGCCATTGCTTCTCAAGGTCCACGTGAACCCGCCTGCAGAGGTCTC
CACCTCCCTGAAGGTGTACCAAGGGCACACCCTGGAGAAGACCTACATGGGGGAAGACTTCTTTTGGGCC
ATCACCCCCACGGCTGGAGACTACATCTTGTTTAAATTTGACAAACCGGTCAACGTGGAGAGTTATTTGT
TCCACAGCGGCAATCAAGAGCACCCAGGAGACATCCTGCTGAACACGACCGTGGATGTTCTCCCTCTTAA
GAGCGACAGTTTGGAAATCAGCAAAGAAACCAAAGACAAACGATTAGAAGATGGCTATTTCAGAATAGGA
AAATTTGAGTATGGTGTTGCAGAGGGAATTGTGGATCCTGGTCTAAACCCTATTTCAGCCTTTCGACTTT
CCGTTATTCAGAACTCAGCTGTTTGGGCCATTCTTAATGAGATTCATATTAAAAAAGTCACCAGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216106 representing NM_173870
Red=Cloning site Green=Tags(s)

MRLRNGTVATALVFVTSFLTLSWYTTWQNGKEKLIAYQREFLALKERLRVAEHRISQRSSELNTIVQQFR
RAGAETNGSKTALSTISDNTIKLLKELTSKKSLRVPSIYYHLPHLLQNERSLQPAVQIGSGRTGVSIVMG
IPTVKREVKSYLVETLHSLIDNLYPEEKLDCVIVVFIGETDLDYVHSVVANLEKEFSREISSGLLEIISP
PESYYPDLTNLKETFGDSKERVRWRTKQNLDYCFLMMYAQEKGIYYIQLEDDIIVKQNYFNTIKNFALQL
SSEEWMILEFSQLGFIGKMFQAPDLALVVEFILMFYKEKPIDWLLDHILWVKVCNPEKDAKHCDRQKANL
RIRFRPSLFQHVGLHSSLSGKIQKLTDKDYMKPLLLKVHVNPPAEVSTSLKVYQGHTLEKTYMGEDFFWA
ITPTAGDYILFKFDKPVNVESYLFHSGNQEHPGDILLNTTVDVLPLKSDSLEISKETKDKRLEDGYFRIG
KFEYGVAEGIVDPGLNPISAFRLSVIQNSAVWAILNEIHIKKVTS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173870
ORF Size 1605 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173870.3, NP_776295.1
RefSeq Size 7054 bp
RefSeq ORF 1608 bp
Locus ID 269181
UniProt ID Q812G0
Cytogenetics 1 B
Summary Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"NM_173870" in other vectors (4)
SKU Description Size Price
MC217662 Mgat4a (untagged) - Mouse mannoside acetylglucosaminyltransferase 4, isoenzyme A (Mgat4a), (10ug) 10 ug
$549.00
MR216106 Mgat4a (Myc-DDK-tagged) - Mouse mannoside acetylglucosaminyltransferase 4, isoenzyme A (Mgat4a) 10 ug
$289.00 $498.00
MR216106L3 Lenti ORF clone of Mgat4a (Myc-DDK-tagged) - Mouse mannoside acetylglucosaminyltransferase 4, isoenzyme A (Mgat4a) 10 ug
$798.00
MR216106L4 Lenti ORF clone of Mgat4a (mGFP-tagged) - Mouse mannoside acetylglucosaminyltransferase 4, isoenzyme A (Mgat4a) 10 ug
$798.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.