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Tmem165 (NM_011626) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216105
Tmem165 (tGFP-tagged) - Mouse transmembrane protein 165 (Tmem165), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Tmem165 or NM_011626"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem165
Synonyms AV026557; pFT27; Tpardl; Tparl
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216105 representing NM_011626
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGGCGCGCCC

ATGGCGGCCGCGGCCCGGGGGAGCGGCCGGGCGCCGACGCGAAGGCTGCTCGTGCTCTTGCTGCTTCAGT
TGCTCTGGGCCCCGGCTGGGGTCCGCGCCGGCCCGGAGGAAGACCTGAGCCATCGGAACCAGGAGCTGCC
GGCGCCCGCCCAGCAGCTGCAGCCGCAGCCCGCGGCGGTGCAGGGCCTCGAGCCGGCCCGGGCCGAGAAA
GGATTGACACCAGTCGCCCCAGTTCATACCAACAAAGAAGATGCAGCTGCCCAGACGAATCTGGGATTTA
TCCATGCGTTTGTGGCTGCCATATCAGTCATCATAGTGTCTGAACTCGGCGACAAGACGTTTTTCATAGC
TGCCATCATGGCGATGCGCTATAACCGGCTGACTGTGCTGGCCGGGGCCATGCTGGCCTTGGCCTTGATG
ACATGCTTGTCGGTTTTGTTTGGCTATGCCACAACAGTCATCCCCAGGGTGTATACATACTATGTTTCAA
CTGCACTCTTCGCCATTTTTGGCATTAGAATGCTTCGGGAAGGTTTGAAGATGAGCCCAGATGAGGGTCA
GGAGGAGCTAGAAGAAGTCCAAGCAGAGTTAAAGAAGAAGGATGAAGAATTCCAACGAACCAAACTCTTA
AATGGGCCAGATGTTGAAACTGGTACAAGCACAGCAATACCTCAGAAAAAGTGGTTACATTTTATTTCAC
CCATTTTTGTTCAAGCGCTCACATTAACGTTCTTGGCAGAATGGGGCGATCGCTCTCAACTCACTACCAT
TGTCCTGGCAGCTAGAGAGGACCCTTATGGTGTAGCAGTGGGTGGCACAGTGGGACACTGCTTATGTACT
GGATTGGCAGTAATTGGAGGAAGGATGATAGCGCAAAAGATCTCTGTCCGAACTGTGACAATCATCGGAG
GCATCGTATTTTTGGCGTTTGCATTTTCTGCCCTGTTTATAAGTCCAGAGTCTGGTTTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216105 representing NM_011626
Red=Cloning site Green=Tags(s)

MAAAARGSGRAPTRRLLVLLLLQLLWAPAGVRAGPEEDLSHRNQELPAPAQQLQPQPAAVQGLEPARAEK
GLTPVAPVHTNKEDAAAQTNLGFIHAFVAAISVIIVSELGDKTFFIAAIMAMRYNRLTVLAGAMLALALM
TCLSVLFGYATTVIPRVYTYYVSTALFAIFGIRMLREGLKMSPDEGQEELEEVQAELKKKDEEFQRTKLL
NGPDVETGTSTAIPQKKWLHFISPIFVQALTLTFLAEWGDRSQLTTIVLAAREDPYGVAVGGTVGHCLCT
GLAVIGGRMIAQKISVRTVTIIGGIVFLAFAFSALFISPESGF

TRTRPLE - GFP Tag - V
Restriction Sites AscI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011626
ORF Size 969 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011626.2, NP_035756.2
RefSeq Size 1896 bp
RefSeq ORF 972 bp
Locus ID 21982
UniProt ID P52875
Cytogenetics 5 40.56 cM
Summary May function as a calcium/proton transporter involved in calcium and in lysosomal pH homeostasis. Therefore, it may play an indirect role in protein glycosylation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011626" in other vectors (4)
SKU Description Size Price
MC209568 Tmem165 (untagged) - Mouse transmembrane protein 165 (Tmem165), (10ug) 10 ug
$300.00
MR216105 Tmem165 (Myc-DDK-tagged) - Mouse transmembrane protein 165 (Tmem165) 10 ug
$289.00 $300.00
MR216105L3 Lenti ORF clone of Tmem165 (Myc-DDK-tagged) - Mouse transmembrane protein 165 (Tmem165) 10 ug
$600.00
MR216105L4 Lenti ORF clone of Tmem165 (mGFP-tagged) - Mouse transmembrane protein 165 (Tmem165) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.