Adam11 (NM_009613) Mouse Tagged ORF Clone

CAT#: MG216099

Adam11 (tGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 11 (Adam11) transcript variant 2, (10ug)

Product Images

Circular map for MG216099

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adam11
• Synonyms: AW060611; Mdc
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216099 representing NM_009613
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGGCGCGCCC
  
  ATGAGGCGGCTGCGGCGCTGGGCGATCGCGGCTCTGCTGCTGTTACCGCTTCTCCCCCCGCCCGGTCTTG
  GGGCCCTGGGTCCCAGAGGAGCTCTGCACTGGAGGAGCTCAGCCCATGTGGGGAGCCCAGAGAGTCCAGA
  GGGCTCTGAGGTCACAGAGCCCAGCCGGCTGGTAAGGCAGAGCTCCGGGGGAGAGGTCCGAAAGCCACAG
  TTGGACACCAGGGTCCGCCAGGATCCGCCCAGGGGGACGCCTGTGCACCTGGCCCAGGTGAGTTTCGTCA
  TCCCGGCCTTCGACTCAAACTTCACTCTGGACCTGGAGCTGAACCATCACCTCCTGTCCTCGCAGTATGT
  GGAGCGCCACTTCAGCCGGGAGGGAACAAGACAACACAGCACTGGGGCTGGAGACCACTGCTACTACCAT
  GGGAAACTCCGGGGCAACCCACAGTCCTTTGCTGCACTCTCTACATGCCAGGGGCTGCATGGGGTCTTCT
  CTGATGGCAACCTGACTTACATCGTAGAGCCTAAGGAGATAGCTGGGCCCTGGGGACCCCCACAGGGACC
  CCTTCCCCACCTCATTTACCGGACCCCTCTCCTCCCAGCCCCCCTTGGATGCAGGGAGCCAGGCTGCCTG
  TTTGCTGTCCCTGCCCAGTCTGCTCTCCCCAACTGGCCCAAGCTAAGAAGGAAAAGGCAGGTCCGCAGGG
  GCCACCCCACAGTGCACAGCGAGACCAAGTATGTGGAGTTGATTGTAATCAATGACCACCAGCTGTTTGA
  GCAGATGCGGCAGTCAGTGGTCCTCACCAGCAACTTTGCAAAATCTGTTGTGAACCTGGCAGATGTGATA
  TACAAGGAACAGCTCAACACAAGAATTGTTCTGGTTGCCATGGAAACGTGGGCAGATGGGGACAAGATCC
  AGGTGCAGGATGACCTACTGGAGACCCTGGCCCGGCTTATGGTCTACCGGCGGGAGGGTCTGCCTGAGCC
  CAGTGATGCCACCCACCTCTTCTCGGGTAGAACCTTCCAGAGCACCAGCAGCGGGGCGGCCTACGTGGGA
  GGCATCTGTTCACTGTCCCGGGGTGGAGGTGTGAACGAGTATGGCAACATGGGTGCCATGGCGGTGACCC
  TGGCCCAGACGCTAGGGCAGAACTTGGGCATGATGTGGAATAAGCACCGGAGCTCAGCAGGGGACTGCAA
  GTGTCCAGACATTTGGCTGGGCTGCATCATGGAGGACACTGGGTTCTATTTGCCCCGCAAGTTCTCGCGC
  TGCAGCATCGACGAATACAACCAGTTTCTGCAGGAGGGAGGCGGGAGCTGCCTGTTCAACAAGCCCCTCA
  AGCTTCTGGACCCTCCCGAGTGCGGAAACGGCTTCGTGGAGGCGGGAGAGGAGTGCGACTGCGGGTCGGT
  GCAGGAGTGCAGCCGAGCAGGGGGCAACTGCTGCAAAAAATGCACCTTGACGCACGACGCCATGTGCAGC
  GATGGGCTCTGTTGTCGCCGCTGCAAGTATGAGCCACGAGGTGTCTCCTGCCGAGAAGCGGTGAATGAGT
  GTGACATTGCAGAGACCTGCACCGGCGACTCAAGCCAGTGTCCCCCTAACCTTCACAAGCTGGACGGTTA
  CTACTGTGATCATGAGCAGGGTCGTTGCTATGGAGGCCGCTGTAAAACCCGGGACCGGCAGTGCCAAGCC
  CTATGGGGCCATGCGGCTGCGGATCGTTTCTGCTATGAGAAGCTGAACGTGGAGGGGACAGAGCGTGGAA
  ACTGTGGACGCAAGGGATCTGGTTGGGTCCAGTGCAGTAAGCAGGATGTGCTCTGTGGCTTCCTTCTGTG
  CGTCAACATCTCTGGAGCTCCGCGGCTAGGGGATCTGGGGGGCGACATCAGCAGTGTCACCTTCTACCAC
  CAGGGCAAGGAGTTGGACTGCAGGGGAGGCCACGTGCAGCTAGCTGATGGCTCGGACCTGAGCTATGTGG
  AGGACGGCACGGCCTGTGGGCCCAACATGTTGTGCCTAGATCACCGCTGCCTGCCAGCCTCTGCCTTCAA
  CTTCAGCACCTGCCCTGGAAGTGGAGAGCGAAGGATCTGCTCCCATCATGGGGTTTGCAGCAACGAGGGG
  AAGTGTATCTGTCAGCCAGACTGGACAGGCAAAGACTGCAGTATTCACAACCCACTGCCCACGTCCCCTC
  CCACTGGGGAGACTGAGAGATACAAAGGTCCCAGCGGTACCAACATCATCATTGGCTCCATCGCCGGGGC
  TGTCCTGGTCGCAGCCATCGTCCTGGGCGGCACGGGCTGGGGATTTAAAAACATCCGTCGTGGAAGGTCC
  GGAGGGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216099 representing NM_009613
  Red=Cloning site Green=Tags(s)
  
  MRRLRRWAIAALLLLPLLPPPGLGALGPRGALHWRSSAHVGSPESPEGSEVTEPSRLVRQSSGGEVRKPQ
  LDTRVRQDPPRGTPVHLAQVSFVIPAFDSNFTLDLELNHHLLSSQYVERHFSREGTRQHSTGAGDHCYYH
  GKLRGNPQSFAALSTCQGLHGVFSDGNLTYIVEPKEIAGPWGPPQGPLPHLIYRTPLLPAPLGCREPGCL
  FAVPAQSALPNWPKLRRKRQVRRGHPTVHSETKYVELIVINDHQLFEQMRQSVVLTSNFAKSVVNLADVI
  YKEQLNTRIVLVAMETWADGDKIQVQDDLLETLARLMVYRREGLPEPSDATHLFSGRTFQSTSSGAAYVG
  GICSLSRGGGVNEYGNMGAMAVTLAQTLGQNLGMMWNKHRSSAGDCKCPDIWLGCIMEDTGFYLPRKFSR
  CSIDEYNQFLQEGGGSCLFNKPLKLLDPPECGNGFVEAGEECDCGSVQECSRAGGNCCKKCTLTHDAMCS
  DGLCCRRCKYEPRGVSCREAVNECDIAETCTGDSSQCPPNLHKLDGYYCDHEQGRCYGGRCKTRDRQCQA
  LWGHAAADRFCYEKLNVEGTERGNCGRKGSGWVQCSKQDVLCGFLLCVNISGAPRLGDLGGDISSVTFYH
  QGKELDCRGGHVQLADGSDLSYVEDGTACGPNMLCLDHRCLPASAFNFSTCPGSGERRICSHHGVCSNEG
  KCICQPDWTGKDCSIHNPLPTSPPTGETERYKGPSGTNIIIGSIAGAVLVAAIVLGGTGWGFKNIRRGRS
  GGA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009613
• ORF Size: 2319 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009613.3, NP_033743.2
• RefSeq Size: 4647 bp
• RefSeq ORF: 2322 bp
• Locus ID: 11488
• UniProt ID: Q9R1V4
• Cytogenetics: 11 66.48 cM
• Gene Summary: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. The protein encoded by this gene is believed to lack metalloproteinase activity due to the lack of a critical catalytic motif. Mice lacking the encoded protein exhibit defects in spatial learning, motor coordination and altered perception of pain. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing. [provided by RefSeq, May 2016]