Naip2 (NM_010872) Mouse Tagged ORF Clone

CAT#: MG216094

Naip2 (tGFP-tagged) - Mouse NLR family apoptosis inhibitory protein 2 (Naip2) transcript variant 1, (10ug)

Product Images

Circular map for MG216094

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Naip2
• Synonyms: Birc1b; Naip-rs6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216094 representing NM_010872
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCCAGGGAGAAGCCGTTGAGGAGATAATTTGTGAGTTTGATGACGACTTGGTCTCTGAGCTCT
  CTACTCTTCTCAGAGTGGATGCACTTTCAGTCTTGAAGAGACAACAAGAAGAAGACCATAAAACACGAAT
  GAAAATGAAGAAAGGTTTTAACTCACAGATGCGCAGTGAAGCCAAACGACTAAAGACTTTTGAGACCTAT
  GACAAGTTCAGATCATGGACGCCACAGGAGATGGCAGCTGCTGGGTTTTACCACACAGGGGTGAAACTTG
  GGGTTCAGTGCTTTTGCTGTAGCTTGATCCTCTTTTCCACCAGACTCAGGAAACTTCCCATAGAGAACCA
  CAAGAAATTACGACCAGAATGTGAGTTCCTTCTGGGCAAAGATGTTGGTAACATTGGCAAGTATGACATC
  CGGGTTAAGAGTCCAGAGAAGATGCTGAGAGGTGACAAAGCAAGGTACCATGAAGAGGAGGCAAGACTGG
  AGTCCTTTGAGGACTGGCCATTTTATGCCCATGGGACATCACCACGTGTACTCTCAGCAGCTGGCTTTGT
  CTTTACAGGTAAAAGGGACACTGTGCAGTGTTTCTCCTGTGGTGGATGCTTGGGCAACTGGGAAGAAGGA
  GATGATCCCTGGAAGGAGCATGCCAAGTGGTTCCCCAAATGTGAATTTCTTCAAAGTAAGAAGTCCCCAG
  AGGAAATTACCCAATATGTACAAAGCTATGAAGGATTTCTTCATGTAACGGGAGAACATTTTGTGAATTC
  CTGGGTCAGAAGAGAATTACCTATGGTATCAGCTTACTGCAATGACAGCGTCTTCGCTAATGAAGAACTA
  AGGATGGACACGTTTAAGGACTGGCCCCACGAATCACCCGGGGCTGTTGAAGCTCTGGTCAAAGCAGGCC
  TTTTCTACACAGGCAAAAGGGACATTGTCCAGTGTTTTTCCTGTGGAGGATGTATGGAGAAGTGGGCAGA
  AGGTGACAACCCAATAGAAGATCACACCAAGTTTTTTCCCAACTGTGTGTTTCTCCAAACCCTGAAGTCC
  TCTGCAGAAGTGATTCCAGCCCTTCAGAGCCACTGTGCACTTCCAGAAGCCATGGAAACCACAAGTGAAA
  GCAACCATGATGATGCAGCAGCAGTTCATTCTACAGTGGTGGATGTTTCTCCAAGTGAAGCCCAGGAACT
  TGAACCTGCATCCAGCTTGGTGTCTGTTCTCTGTAGAGACCAGGACCACAGTGAAGCCCAAGGGCGTGGA
  TGTGCATCCAGTGGGACCTATCTTCCCTCCACAGACTTGGGTCAGAGTGAAGCCCAGTGGCTTCAAGAGG
  CCAGGAGTCTGAGTGAGCAGCTGAGAGACACCTACACCAAAGCTACTTTCCGCCACATGAACTTGCCAGA
  AGTGTACTCCAGCCTCGGCACTGACCATTTGCTCAGCTGCGATGTGTCCATCATTTCAAAGCACATCAGC
  CAGCCAGTGCAAGGGTCCCTGACGATCCCTGAGGTCTTCTCCAATCTCAACTCTGTCATGTGTGTGGAGG
  GGGAAGCTGGCAGTGGAAAGACAACCTTCCTGAAGAGGATAGCTTTTCTCTGGGCATCAGGATGCTGCCC
  CCTGTTGTACAGGTTCCAGCTGGTCTTCTACCTCTCCCTTAGTTCCATCACACCAGGCCAGGAACTGGCC
  AAAATCATCTGTGCCCAACTCCTAGGGGCAGGAGGCTGCATTAGTGAAGTGTGTCTGAGCAGCATCATCC
  AGCAGTTACAACACCAGGTGCTGTTCCTGTTGGATGACTACAGTGGGCTGGCCTCACTCCCCCAAGCCCT
  ACACACACTGATTACAAAAAACTACTTGTCACGGACCTGCTTATTGATCGCTGTGCATACAAACAAGGTC
  AGGGGCATCCGCCCATACCTAGATACGAGTCTAGAGATCAAAGAGTTTCCCTTCTATAATACTGTCTCTG
  TGTTACGGAAGCTTTTTTCACATGACATAATGCGTGTACGAAAATTTATAAATTACTTTGGATTTCATGA
  AGAGTTGCAGGGAATTCACAAGACACCCCTCTTCGTGGCAGCAGTATGTACTGACTGGTTTAAAAATCCA
  TCTGACCAGCCCTTTCAGGATGTGGCACTTTTCAAGGCCTATATGCAATACTTGTCCTTAAAGCACAAAG
  GTGCAGCTAAGCCTCTCCAGGCCACAGTGTCCTCATGTGGGCAGCTGGCCTTGACAGGGCTTTTCTCATC
  GTGCTTTGAGTTCAATAGTGATAACCTGGCAGAGGCAGGAGTTGATGAAGATGAAGAGCTCACCACCTGC
  TTGATGAGCAAATTCACCGCCCAGAGACTGAGGCCAGTCTACCGGTTTTTAGGTCCTCTGTTCCAGGAGT
  TTCTTGCTGCCGTGAGGTTGACTGAACTCCTGAGTTCAGATAGGCAGGAAGACCAAGATCTGGGACTTTA
  TTATTTGAGACAAATTAACTCACCTCTAAAGGCTATGAGCATCTACCACACTTTTTTGAAATATGTCTCC
  AGCCACCCTTCATCAAAGGCAGCGCCAACAGTTGTATCTCATTTGCTTCAGTTGGTGGATGAGAAAGAGT
  CACTGGAGAACATGTCTGAAAATGAGGATTACATGAAGCTCCATCCAGAAGCTTTGCTGTGGATTGAGTG
  TCTTAGAGGATTGTGGCAGCTGTCTCCTGAATCTTTTTCTTTGTTCATTTCAGAAAACTTATTGCGCATT
  TGTCTAAACTTTGCTCATGAAAGCAACACAGTTGCTGCATGTTCTCCAGTTATTTTGCAGTTCCTTCGAG
  GGAGAACACTGGATTTGAAAGTTTTGAGTTTACAGTACTTTTGGGACCATCCAGAAACCTTGTTGTTATT
  GAAGAGTATTAAGATCTCATTAAATGGAAATAACTGGGTACAGAGAATCGATTTTTCACTCATAGAAAAA
  TCTTTTGAAAAAGTACAGCCACCAACTATAGATCAGGACTATGCCATTGCCTTTCAACCTATAAATGAAG
  TCCAGAAAAATTTATCTGAAAAGAAACACATAATAAAGAAGTATGAGGATATGAAACACCAGATCCCACT
  AAATATTAGTACTGGCTACTGGAAACTGTCCCCCAAGCCATACAAGATCCCCAAGCTGGAAGTTCAAGTG
  ACCAACACAGGTCCAGCAGATCAAGCACTGCTCCAGGTCCTCATGGAAGTCTTCTCAGCTTCACAGAGTA
  TTGAGTTCCGTTTATCCGACAGCAGTGGCTTCCTTGAAAGCATCCGCCCAGCTCTGGAGCTGAGTAAGGC
  CTCTGTCACCAAGTGTTCCATGTCCAGGCTGGAGCTCAGCAGAGAAGATCAGAAGCTGCTTCTCACCCTG
  CCTACCCTGCAGTCTCTCGAGGTCTCAGAGACAAACCAGTTACCAGATCAGCTCTTCCATAACTTGCACA
  AGTTCCTGGGCCTGAAAGAACTGTGTGTGAGACTAGATAGCAAGCCGGATGTGCTCTCAGTCCTTCCTGG
  AGAGTTCCCAAACCTCCATCACATGGAGAAGTTATCCATCAGAACCTCCACGGAGTCTGACCTCTCCAAA
  CTAGTTAAGTTGATTCAGAACTCTCCAAATCTCCATGTTTTCCATCTGAAGTGTAATTTCCTTTCGAATT
  GTGAGCCTCTCATGACTGTGCTTGCTTCCTGCAAGAAACTCAGAGAGATTGAGTTTTCTGGACGATGCTT
  TGAAGCCATGACCTTTGTCAACATTTTGCCAAATTTTGTTTTTCTGAAGATATTGAATCTTAGAGACCAA
  CAATTCCCAGATAAAGAAACATCAGAAAAGTTTGCCCAGGCTCTGGGTTCTCTCAGGAACCTGGAGAAAC
  TGTTCGTTCCCACTGGGGACGGGATTCACCAAGTGGCCAAACTGATTGTCCGGCAGTGTCTGCAGCTCCC
  ATGTCTCCGAGTTCTTGTCTTTGCAGAGACTCTGGATGACGACAGTGTACTGGAAATTGCCAAGGGAGCA
  ACCAGGGGAGGCTTCCAAAAACTTGAGAACTTAGACCTTACACTGAATCACAAGATTACAGAGGAAGGAT
  ACAGAAATTTCTTTCAAGTCCTGGACAACCTGCCAAACCTGAAAAACCTGGACATTTCCAGGCATATCCC
  AGAATGCATTCAAATTCAGGCCATCACTGTCAAGGCTCTGGGTCAGTGTGTGTCCCGACTGCCCAGCCTC
  ACCAGGCTCGGGATGCTCAGTTGGCTCCTGGATGAAGAGGACATAAAAGTGATTAATGATGTGAAGGAAA
  GACACCCTCAGTCCAAACGCTTGACTGTTCACTGGAGGTGGGTAGTGCCATTCTCTCCTGTCATTCAGAA
  G
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216094 representing NM_010872
  Red=Cloning site Green=Tags(s)
  
  MAAQGEAVEEIICEFDDDLVSELSTLLRVDALSVLKRQQEEDHKTRMKMKKGFNSQMRSEAKRLKTFETY
  DKFRSWTPQEMAAAGFYHTGVKLGVQCFCCSLILFSTRLRKLPIENHKKLRPECEFLLGKDVGNIGKYDI
  RVKSPEKMLRGDKARYHEEEARLESFEDWPFYAHGTSPRVLSAAGFVFTGKRDTVQCFSCGGCLGNWEEG
  DDPWKEHAKWFPKCEFLQSKKSPEEITQYVQSYEGFLHVTGEHFVNSWVRRELPMVSAYCNDSVFANEEL
  RMDTFKDWPHESPGAVEALVKAGLFYTGKRDIVQCFSCGGCMEKWAEGDNPIEDHTKFFPNCVFLQTLKS
  SAEVIPALQSHCALPEAMETTSESNHDDAAAVHSTVVDVSPSEAQELEPASSLVSVLCRDQDHSEAQGRG
  CASSGTYLPSTDLGQSEAQWLQEARSLSEQLRDTYTKATFRHMNLPEVYSSLGTDHLLSCDVSIISKHIS
  QPVQGSLTIPEVFSNLNSVMCVEGEAGSGKTTFLKRIAFLWASGCCPLLYRFQLVFYLSLSSITPGQELA
  KIICAQLLGAGGCISEVCLSSIIQQLQHQVLFLLDDYSGLASLPQALHTLITKNYLSRTCLLIAVHTNKV
  RGIRPYLDTSLEIKEFPFYNTVSVLRKLFSHDIMRVRKFINYFGFHEELQGIHKTPLFVAAVCTDWFKNP
  SDQPFQDVALFKAYMQYLSLKHKGAAKPLQATVSSCGQLALTGLFSSCFEFNSDNLAEAGVDEDEELTTC
  LMSKFTAQRLRPVYRFLGPLFQEFLAAVRLTELLSSDRQEDQDLGLYYLRQINSPLKAMSIYHTFLKYVS
  SHPSSKAAPTVVSHLLQLVDEKESLENMSENEDYMKLHPEALLWIECLRGLWQLSPESFSLFISENLLRI
  CLNFAHESNTVAACSPVILQFLRGRTLDLKVLSLQYFWDHPETLLLLKSIKISLNGNNWVQRIDFSLIEK
  SFEKVQPPTIDQDYAIAFQPINEVQKNLSEKKHIIKKYEDMKHQIPLNISTGYWKLSPKPYKIPKLEVQV
  TNTGPADQALLQVLMEVFSASQSIEFRLSDSSGFLESIRPALELSKASVTKCSMSRLELSREDQKLLLTL
  PTLQSLEVSETNQLPDQLFHNLHKFLGLKELCVRLDSKPDVLSVLPGEFPNLHHMEKLSIRTSTESDLSK
  LVKLIQNSPNLHVFHLKCNFLSNCEPLMTVLASCKKLREIEFSGRCFEAMTFVNILPNFVFLKILNLRDQ
  QFPDKETSEKFAQALGSLRNLEKLFVPTGDGIHQVAKLIVRQCLQLPCLRVLVFAETLDDDSVLEIAKGA
  TRGGFQKLENLDLTLNHKITEEGYRNFFQVLDNLPNLKNLDISRHIPECIQIQAITVKALGQCVSRLPSL
  TRLGMLSWLLDEEDIKVINDVKERHPQSKRLTVHWRWVVPFSPVIQK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010872
• ORF Size: 4341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010872.3, NP_035002.2
• RefSeq Size: 4823 bp
• RefSeq ORF: 4344 bp
• Locus ID: 17948
• UniProt ID: Q9QUK4
• Cytogenetics: 13 53.01 cM
• Gene Summary: Sensor component of the NLRC4 inflammasome that specifically recognizes and binds type III secretion system (T3SS) rod proteins such as S.typhimurium (Salmonella) PrgJ and B.thailandensis BsaK from pathogenic bacteria. Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri. Prevents motor-neuron apoptosis induced by a variety of signals.[UniProtKB/Swiss-Prot Function]