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Naip2 (NM_010872) Mouse Tagged ORF Clone

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SKU
MG216094
Naip2 (tGFP-tagged) - Mouse NLR family apoptosis inhibitory protein 2 (Naip2) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,438.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

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Other products for "Naip2 or NM_010872"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Naip2
Synonyms Birc1b; Naip-rs6
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216094 representing NM_010872
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCCCAGGGAGAAGCCGTTGAGGAGATAATTTGTGAGTTTGATGACGACTTGGTCTCTGAGCTCT
CTACTCTTCTCAGAGTGGATGCACTTTCAGTCTTGAAGAGACAACAAGAAGAAGACCATAAAACACGAAT
GAAAATGAAGAAAGGTTTTAACTCACAGATGCGCAGTGAAGCCAAACGACTAAAGACTTTTGAGACCTAT
GACAAGTTCAGATCATGGACGCCACAGGAGATGGCAGCTGCTGGGTTTTACCACACAGGGGTGAAACTTG
GGGTTCAGTGCTTTTGCTGTAGCTTGATCCTCTTTTCCACCAGACTCAGGAAACTTCCCATAGAGAACCA
CAAGAAATTACGACCAGAATGTGAGTTCCTTCTGGGCAAAGATGTTGGTAACATTGGCAAGTATGACATC
CGGGTTAAGAGTCCAGAGAAGATGCTGAGAGGTGACAAAGCAAGGTACCATGAAGAGGAGGCAAGACTGG
AGTCCTTTGAGGACTGGCCATTTTATGCCCATGGGACATCACCACGTGTACTCTCAGCAGCTGGCTTTGT
CTTTACAGGTAAAAGGGACACTGTGCAGTGTTTCTCCTGTGGTGGATGCTTGGGCAACTGGGAAGAAGGA
GATGATCCCTGGAAGGAGCATGCCAAGTGGTTCCCCAAATGTGAATTTCTTCAAAGTAAGAAGTCCCCAG
AGGAAATTACCCAATATGTACAAAGCTATGAAGGATTTCTTCATGTAACGGGAGAACATTTTGTGAATTC
CTGGGTCAGAAGAGAATTACCTATGGTATCAGCTTACTGCAATGACAGCGTCTTCGCTAATGAAGAACTA
AGGATGGACACGTTTAAGGACTGGCCCCACGAATCACCCGGGGCTGTTGAAGCTCTGGTCAAAGCAGGCC
TTTTCTACACAGGCAAAAGGGACATTGTCCAGTGTTTTTCCTGTGGAGGATGTATGGAGAAGTGGGCAGA
AGGTGACAACCCAATAGAAGATCACACCAAGTTTTTTCCCAACTGTGTGTTTCTCCAAACCCTGAAGTCC
TCTGCAGAAGTGATTCCAGCCCTTCAGAGCCACTGTGCACTTCCAGAAGCCATGGAAACCACAAGTGAAA
GCAACCATGATGATGCAGCAGCAGTTCATTCTACAGTGGTGGATGTTTCTCCAAGTGAAGCCCAGGAACT
TGAACCTGCATCCAGCTTGGTGTCTGTTCTCTGTAGAGACCAGGACCACAGTGAAGCCCAAGGGCGTGGA
TGTGCATCCAGTGGGACCTATCTTCCCTCCACAGACTTGGGTCAGAGTGAAGCCCAGTGGCTTCAAGAGG
CCAGGAGTCTGAGTGAGCAGCTGAGAGACACCTACACCAAAGCTACTTTCCGCCACATGAACTTGCCAGA
AGTGTACTCCAGCCTCGGCACTGACCATTTGCTCAGCTGCGATGTGTCCATCATTTCAAAGCACATCAGC
CAGCCAGTGCAAGGGTCCCTGACGATCCCTGAGGTCTTCTCCAATCTCAACTCTGTCATGTGTGTGGAGG
GGGAAGCTGGCAGTGGAAAGACAACCTTCCTGAAGAGGATAGCTTTTCTCTGGGCATCAGGATGCTGCCC
CCTGTTGTACAGGTTCCAGCTGGTCTTCTACCTCTCCCTTAGTTCCATCACACCAGGCCAGGAACTGGCC
AAAATCATCTGTGCCCAACTCCTAGGGGCAGGAGGCTGCATTAGTGAAGTGTGTCTGAGCAGCATCATCC
AGCAGTTACAACACCAGGTGCTGTTCCTGTTGGATGACTACAGTGGGCTGGCCTCACTCCCCCAAGCCCT
ACACACACTGATTACAAAAAACTACTTGTCACGGACCTGCTTATTGATCGCTGTGCATACAAACAAGGTC
AGGGGCATCCGCCCATACCTAGATACGAGTCTAGAGATCAAAGAGTTTCCCTTCTATAATACTGTCTCTG
TGTTACGGAAGCTTTTTTCACATGACATAATGCGTGTACGAAAATTTATAAATTACTTTGGATTTCATGA
AGAGTTGCAGGGAATTCACAAGACACCCCTCTTCGTGGCAGCAGTATGTACTGACTGGTTTAAAAATCCA
TCTGACCAGCCCTTTCAGGATGTGGCACTTTTCAAGGCCTATATGCAATACTTGTCCTTAAAGCACAAAG
GTGCAGCTAAGCCTCTCCAGGCCACAGTGTCCTCATGTGGGCAGCTGGCCTTGACAGGGCTTTTCTCATC
GTGCTTTGAGTTCAATAGTGATAACCTGGCAGAGGCAGGAGTTGATGAAGATGAAGAGCTCACCACCTGC
TTGATGAGCAAATTCACCGCCCAGAGACTGAGGCCAGTCTACCGGTTTTTAGGTCCTCTGTTCCAGGAGT
TTCTTGCTGCCGTGAGGTTGACTGAACTCCTGAGTTCAGATAGGCAGGAAGACCAAGATCTGGGACTTTA
TTATTTGAGACAAATTAACTCACCTCTAAAGGCTATGAGCATCTACCACACTTTTTTGAAATATGTCTCC
AGCCACCCTTCATCAAAGGCAGCGCCAACAGTTGTATCTCATTTGCTTCAGTTGGTGGATGAGAAAGAGT
CACTGGAGAACATGTCTGAAAATGAGGATTACATGAAGCTCCATCCAGAAGCTTTGCTGTGGATTGAGTG
TCTTAGAGGATTGTGGCAGCTGTCTCCTGAATCTTTTTCTTTGTTCATTTCAGAAAACTTATTGCGCATT
TGTCTAAACTTTGCTCATGAAAGCAACACAGTTGCTGCATGTTCTCCAGTTATTTTGCAGTTCCTTCGAG
GGAGAACACTGGATTTGAAAGTTTTGAGTTTACAGTACTTTTGGGACCATCCAGAAACCTTGTTGTTATT
GAAGAGTATTAAGATCTCATTAAATGGAAATAACTGGGTACAGAGAATCGATTTTTCACTCATAGAAAAA
TCTTTTGAAAAAGTACAGCCACCAACTATAGATCAGGACTATGCCATTGCCTTTCAACCTATAAATGAAG
TCCAGAAAAATTTATCTGAAAAGAAACACATAATAAAGAAGTATGAGGATATGAAACACCAGATCCCACT
AAATATTAGTACTGGCTACTGGAAACTGTCCCCCAAGCCATACAAGATCCCCAAGCTGGAAGTTCAAGTG
ACCAACACAGGTCCAGCAGATCAAGCACTGCTCCAGGTCCTCATGGAAGTCTTCTCAGCTTCACAGAGTA
TTGAGTTCCGTTTATCCGACAGCAGTGGCTTCCTTGAAAGCATCCGCCCAGCTCTGGAGCTGAGTAAGGC
CTCTGTCACCAAGTGTTCCATGTCCAGGCTGGAGCTCAGCAGAGAAGATCAGAAGCTGCTTCTCACCCTG
CCTACCCTGCAGTCTCTCGAGGTCTCAGAGACAAACCAGTTACCAGATCAGCTCTTCCATAACTTGCACA
AGTTCCTGGGCCTGAAAGAACTGTGTGTGAGACTAGATAGCAAGCCGGATGTGCTCTCAGTCCTTCCTGG
AGAGTTCCCAAACCTCCATCACATGGAGAAGTTATCCATCAGAACCTCCACGGAGTCTGACCTCTCCAAA
CTAGTTAAGTTGATTCAGAACTCTCCAAATCTCCATGTTTTCCATCTGAAGTGTAATTTCCTTTCGAATT
GTGAGCCTCTCATGACTGTGCTTGCTTCCTGCAAGAAACTCAGAGAGATTGAGTTTTCTGGACGATGCTT
TGAAGCCATGACCTTTGTCAACATTTTGCCAAATTTTGTTTTTCTGAAGATATTGAATCTTAGAGACCAA
CAATTCCCAGATAAAGAAACATCAGAAAAGTTTGCCCAGGCTCTGGGTTCTCTCAGGAACCTGGAGAAAC
TGTTCGTTCCCACTGGGGACGGGATTCACCAAGTGGCCAAACTGATTGTCCGGCAGTGTCTGCAGCTCCC
ATGTCTCCGAGTTCTTGTCTTTGCAGAGACTCTGGATGACGACAGTGTACTGGAAATTGCCAAGGGAGCA
ACCAGGGGAGGCTTCCAAAAACTTGAGAACTTAGACCTTACACTGAATCACAAGATTACAGAGGAAGGAT
ACAGAAATTTCTTTCAAGTCCTGGACAACCTGCCAAACCTGAAAAACCTGGACATTTCCAGGCATATCCC
AGAATGCATTCAAATTCAGGCCATCACTGTCAAGGCTCTGGGTCAGTGTGTGTCCCGACTGCCCAGCCTC
ACCAGGCTCGGGATGCTCAGTTGGCTCCTGGATGAAGAGGACATAAAAGTGATTAATGATGTGAAGGAAA
GACACCCTCAGTCCAAACGCTTGACTGTTCACTGGAGGTGGGTAGTGCCATTCTCTCCTGTCATTCAGAA
G

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216094 representing NM_010872
Red=Cloning site Green=Tags(s)

MAAQGEAVEEIICEFDDDLVSELSTLLRVDALSVLKRQQEEDHKTRMKMKKGFNSQMRSEAKRLKTFETY
DKFRSWTPQEMAAAGFYHTGVKLGVQCFCCSLILFSTRLRKLPIENHKKLRPECEFLLGKDVGNIGKYDI
RVKSPEKMLRGDKARYHEEEARLESFEDWPFYAHGTSPRVLSAAGFVFTGKRDTVQCFSCGGCLGNWEEG
DDPWKEHAKWFPKCEFLQSKKSPEEITQYVQSYEGFLHVTGEHFVNSWVRRELPMVSAYCNDSVFANEEL
RMDTFKDWPHESPGAVEALVKAGLFYTGKRDIVQCFSCGGCMEKWAEGDNPIEDHTKFFPNCVFLQTLKS
SAEVIPALQSHCALPEAMETTSESNHDDAAAVHSTVVDVSPSEAQELEPASSLVSVLCRDQDHSEAQGRG
CASSGTYLPSTDLGQSEAQWLQEARSLSEQLRDTYTKATFRHMNLPEVYSSLGTDHLLSCDVSIISKHIS
QPVQGSLTIPEVFSNLNSVMCVEGEAGSGKTTFLKRIAFLWASGCCPLLYRFQLVFYLSLSSITPGQELA
KIICAQLLGAGGCISEVCLSSIIQQLQHQVLFLLDDYSGLASLPQALHTLITKNYLSRTCLLIAVHTNKV
RGIRPYLDTSLEIKEFPFYNTVSVLRKLFSHDIMRVRKFINYFGFHEELQGIHKTPLFVAAVCTDWFKNP
SDQPFQDVALFKAYMQYLSLKHKGAAKPLQATVSSCGQLALTGLFSSCFEFNSDNLAEAGVDEDEELTTC
LMSKFTAQRLRPVYRFLGPLFQEFLAAVRLTELLSSDRQEDQDLGLYYLRQINSPLKAMSIYHTFLKYVS
SHPSSKAAPTVVSHLLQLVDEKESLENMSENEDYMKLHPEALLWIECLRGLWQLSPESFSLFISENLLRI
CLNFAHESNTVAACSPVILQFLRGRTLDLKVLSLQYFWDHPETLLLLKSIKISLNGNNWVQRIDFSLIEK
SFEKVQPPTIDQDYAIAFQPINEVQKNLSEKKHIIKKYEDMKHQIPLNISTGYWKLSPKPYKIPKLEVQV
TNTGPADQALLQVLMEVFSASQSIEFRLSDSSGFLESIRPALELSKASVTKCSMSRLELSREDQKLLLTL
PTLQSLEVSETNQLPDQLFHNLHKFLGLKELCVRLDSKPDVLSVLPGEFPNLHHMEKLSIRTSTESDLSK
LVKLIQNSPNLHVFHLKCNFLSNCEPLMTVLASCKKLREIEFSGRCFEAMTFVNILPNFVFLKILNLRDQ
QFPDKETSEKFAQALGSLRNLEKLFVPTGDGIHQVAKLIVRQCLQLPCLRVLVFAETLDDDSVLEIAKGA
TRGGFQKLENLDLTLNHKITEEGYRNFFQVLDNLPNLKNLDISRHIPECIQIQAITVKALGQCVSRLPSL
TRLGMLSWLLDEEDIKVINDVKERHPQSKRLTVHWRWVVPFSPVIQK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010872
ORF Size 4341 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010872.3, NP_035002.2
RefSeq Size 4823 bp
RefSeq ORF 4344 bp
Locus ID 17948
UniProt ID Q9QUK4
Cytogenetics 13 53.01 cM
Summary Sensor component of the NLRC4 inflammasome that specifically recognizes and binds type III secretion system (T3SS) rod proteins such as S.typhimurium (Salmonella) PrgJ and B.thailandensis BsaK from pathogenic bacteria. Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri. Prevents motor-neuron apoptosis induced by a variety of signals.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010872" in other vectors (4)
SKU Description Size Price
MC224481 Naip2 (untagged) - Mouse NLR family, apoptosis inhibitory protein 2 (Naip2), transcript variant 1, (10ug) 10 ug
$1,362.00
MR216094 Naip2 (Myc-DDK-tagged) - Mouse NLR family, apoptosis inhibitory protein 2 (Naip2), transcript variant 1 10 ug
$589.00 $1,238.00
MR216094L3 Lenti ORF clone of Naip2 (Myc-DDK-tagged) - Mouse NLR family, apoptosis inhibitory protein 2 (Naip2), transcript variant 1 10 ug
$1,538.00
MR216094L4 Lenti ORF clone of Naip2 (mGFP-tagged) - Mouse NLR family, apoptosis inhibitory protein 2 (Naip2), transcript variant 1 10 ug
$1,538.00

file-alt Citations

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