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Slc39a8 (NM_001135150) Mouse Tagged ORF Clone

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SKU
MG216081
Slc39a8 (tGFP-tagged) - Mouse solute carrier family 39 (metal ion transporter) member 8 (Slc39a8) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc39a8
Synonyms 4933419D20Rik; AA986696; BIGM103; ZIP-8; Zip8
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216081 representing NM_001135150
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCCGGGCCGGGCAGTGGCCGGGCTGCTGCTGCTGGCGGCCACCAGCCTGGGACACCCCTCGGAGG
GACCGGAGCTCGCCTTCAGTGAGGATGTGCTGAGCGTGTTCGGCGCCAACCGGAGCCTGTCGGCCGCGCA
GCTGGGGCGCCTGCTGGAGCGTCTGGGGGCTGCATCCCAGCAGGGAGCGCTGGACCTGGGCCAGCTGCAC
TTCAACCAGTGTTTGTCAGCAGAAGACATCTTTTCTCTTCATGGTTTCTCAAATGTCACCCAGATAACCA
GCTCGAACTTCTCTGCCATCTGCCCCGCGATCTTACAGCAGCTGAACTTCCATCCCTGCGAGGATCTAAG
AAAGCACAACGCAAAGCCCAGTCTTTCCGAAGTCTGGGGCTATGGATTCCTGTCAGTGACAATTATCAAT
CTGGCCTCTCTCCTGGGATTGATTTTAACCCCCTTGATAAAGAAGTCTTATTTCCCCAAGATTTTAACTT
ATTTCGTGGGACTCGCTATTGGGACTCTTTTCTCAAACGCCATTTTCCAGCTTATTCCAGAGGCATTTGG
ATTTAATCCCAAAATTGACAATTACGTTGAGAAAGCAGTTGCTGTGTTTGGTGGATTTTACATGCTTTTC
TTTGTCGAGAGAACACTTAAGATGCTACTGAAAACATATGGCCAGAATGACCATACTCACTTCAGGAATG
ATGACTTTGGTTCTAAAGAAAAAACCCACCAACCCAAAACGTTACCATTGCCTGCAGTCAACGGTGTGAC
TTGCTATGCCAACCCCGCTGTCACTGAGCCTAACGGACACATCCACTTCGACACTGTCAGCGTTGTATCC
CTCCAGGATGGAAAAACAGAGCCAAGCTCATGTACCTGTCTGAAGGGGCCCAAACTGTCAGAAATAGGAA
CAATTGCCTGGATGATCACGCTTTGTGATGCCCTCCACAATTTCATCGACGGCTTGGCGATTGGGGCATC
TTGTACTTTGTCTCTTCTTCAGGGGCTCAGTACGTCCATAGCGATCCTGTGTGAGGAGTTTCCTCATGAG
TTAGGGGACTTTGTGATCTTGCTCAATGCAGGAATGAGCACCCGGCAAGCCTTGCTGTTCAACTTCCTCT
CCGCGTGTTCCTGCTACGTGGGACTAGCTTTCGGCATTTTGGTGGGCAACAATTTTGCTCCCAATATTAT
ATTTGCACTCGCTGGAGGCATGTTCCTCTACATTTCTCTGGCAGATATGTTTCCAGAGATGAACGACATG
CTGAGAGAAAAAGTAACTGGAAGACAAACGGATTTCACCTTCTTCATGATCCAGAATGCAGGGATGTTAA
CCGGCTTCACTGCCATCCTGCTCATCACCTTGTACGCAGGAGACATCGAATTGCAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216081 representing NM_001135150
Red=Cloning site Green=Tags(s)

MAPGRAVAGLLLLAATSLGHPSEGPELAFSEDVLSVFGANRSLSAAQLGRLLERLGAASQQGALDLGQLH
FNQCLSAEDIFSLHGFSNVTQITSSNFSAICPAILQQLNFHPCEDLRKHNAKPSLSEVWGYGFLSVTIIN
LASLLGLILTPLIKKSYFPKILTYFVGLAIGTLFSNAIFQLIPEAFGFNPKIDNYVEKAVAVFGGFYMLF
FVERTLKMLLKTYGQNDHTHFRNDDFGSKEKTHQPKTLPLPAVNGVTCYANPAVTEPNGHIHFDTVSVVS
LQDGKTEPSSCTCLKGPKLSEIGTIAWMITLCDALHNFIDGLAIGASCTLSLLQGLSTSIAILCEEFPHE
LGDFVILLNAGMSTRQALLFNFLSACSCYVGLAFGILVGNNFAPNIIFALAGGMFLYISLADMFPEMNDM
LREKVTGRQTDFTFFMIQNAGMLTGFTAILLITLYAGDIELQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001135150
ORF Size 1386 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001135150.1, NP_001128622.1
RefSeq Size 3533 bp
RefSeq ORF 1389 bp
Locus ID 67547
UniProt ID Q91W10
Cytogenetics 3 63.04 cM
Summary Acts as a manganese and zinc influx transporter. Plays a role in manganese reabsorption in the proximal tubule of the kidney and in manganese uptake into the brain.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001135150" in other vectors (4)
SKU Description Size Price
MC216319 Slc39a8 (untagged) - Mouse solute carrier family 39 (metal ion transporter), member 8 (Slc39a8), transcript variant 1, (10ug) 10 ug
$503.00
MR216081 Slc39a8 (Myc-DDK-tagged) - Mouse solute carrier family 39 (metal ion transporter), member 8 (Slc39a8), transcript variant 1 10 ug
$289.00 $457.00
MR216081L3 Lenti ORF clone of Slc39a8 (Myc-DDK-tagged) - Mouse solute carrier family 39 (metal ion transporter), member 8 (Slc39a8), transcript variant 1 10 ug
$757.00
MR216081L4 Lenti ORF clone of Slc39a8 (mGFP-tagged) - Mouse solute carrier family 39 (metal ion transporter), member 8 (Slc39a8), transcript variant 1 10 ug
$757.00

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