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Rrbp1 (NM_133626) Mouse Tagged ORF Clone

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SKU
MG216072
Rrbp1 (tGFP-tagged) - Mouse ribosome binding protein 1 (Rrbp1) transcript variant 2, (10ug). Note: ORF is codon optimized
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $842.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rrbp1
Synonyms 1700087N07Rik; 5730465C04Rik; ES/130; mKIAA1398; mRRp0; mRRp1.8; mRRp10; mRRp15a; mRRp15b
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216072 representing NM_133626, codon optimized.
Due to the complexity of NM_133626, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATATATACGACACGCAGACTCTTGGCGTGGTAGTGTTCGGAGGATTTATGGTAGTGAGTGCGATAG
GAATCTTCCTCGTTTCTACATTCAGTATGAAAGAGACATCCTACGAGGAAGCGCTTGCCAACCAGCGAAA
GGAGATGGCTAAGACACATCATCAGAAAGGCGAGAAAAAAAAAAAGGAGAAGACAGTCGAGAAGAAAGGT
AAAACCAAAAAAAAGGAGGAGAAGCCAAATGGAAAGATTCCTGAGCATGACTTGGATCCTAATGTCACTA
TCATACTGAAGGAGCCAGTGAGAGTCTCTGCCGTCGCCGTCGCACCTACCTCTGTGCACTCATCTGTCGG
ACATACACCGATCGCAACGGTACCAGCGATGCCACAGGAAAAACTGGCGTCTTCACCAAAGGATAGGAAA
AAGAAAGAGAAGAAAGTGGCAAAAGTTGAACCTGCCGTGTCCAGCATCGTCAATTCAATACAGGTCTTGG
CCAGCAAGTCTGCCATCCTCGAGGCGACTCCAAAGGAGGTGCCAATGGTTGCTGTACCGCCAGTCGGTTC
CAAGGCCTCAAGTCCAGCCACCAGCTCTCAAGGCAAGAAAGGACAAGGAGCACAGAATCAGGCCAAGAAA
GGCGAAGGAGCACAAAACCAAGGTAAGAAAGGAGAAGGCGCGCAGAATCAGGCGAAAAAAGGCGAAGGAG
CTCAGAACCAGGCTAAAAAAGGTGAAGGTGCTCAGAACCAAGGCAAAAAAGGAGAAGGCGCTCAGAATCA
AGCAAAGAAAGGCGAAGGCGGCCAAAATCAGGCCAAGAAAGGAGAAGGCGCTCAGAACCAAGGAAAAAAA
GGTGAAGGAGCACAGAACCAAGGCAAAAAAGGCGAAGGTGCACAGAATCAGGCAAAGAAAGGAGAAGGAG
CGCAGAACCAGGCCAAAAAAGGTGAAGGTGCTCAGAACCAAGGCAAAAAAGGCGAAGGCGCTCAAAACCA
GAGCAAAAAAGGAGAAGGAGCTCAGAACCAGGCGAAGAAAGGAGAAGGTGGCCAAAACCAGGCTAAAAAA
GGAGAAGGTGCACAGAACCAGGCTAAAAAAGGCGAAGGAGCGCAGAATCAGGCCAAGAAAGGAGAAGGAG
TGCAGAATCAGGCCAAAAAAGGAGTCGAAGGTGCACAGAATCAAGGAAAGAAAGGAGAAGCTAATCAGAA
TCAGGCAAAAAAAGGTGAAGGCGGTCAGAACCAAACAAAGAAAGGTGAAGGACCGCAGAACCAAGGAAAA
AAAGGCGAGGCAGCGCAGAAGCAGGATAAAAAAATAGAAGGAGCTCAGAACCAAGGCAAGAAACCAGAAG
GAACAAGCAATCAAGGCAAGAAAGGTGAAGGCGCTCAGAATCAAGGTAAAAAAGGTGAAGGAGCACAGAA
TCAAGGAAAGAAAGGCGAAGGAGCACAAAATCAAGGAAAGAAAGGCGAAGGAGCACAAAATCAAGGTAAA
AAAGGCGAAGGAGCTCAAAACCAAGGTAAGAAAGGAGAAGGCGCACAGAATCAAGGCAAGAAAGGAGAAG
GCGCACAGAATCAAGGTAAGAAAGGCGAAGGTCCACAGAACCAAGCCAAGAAAGGAGAAGGAGCTCAGAA
TCAAGGTAAGAAAGGAGAAGGAGCACAGAATCAAGGCAAGAAAGGAGAAGGAGCGCAGAACCAAGGAAAG
AAAGCCGAAGGAGTGCAAAGCCAGTCCAAGAAAGGTGAAGGAACACAAAACCAAGGTAAGAAAGGAGACG
GCAATCCAAACCAAGGCAAGAAAGGCGAAGGTGCTTCTAACCAGAATAGAAAAACGGACACTGTGGCTAA
CCAAGGCACTAAGCAGGAAGGCGTGTCAAACCAGGTTAAAAAGTCCGAAGGAAGTCCTAACCAAGGAAAA
AAGGCGGAAGGCGCGCCAAACCAAGGAAAAAAAAAAGACGGCTCTCCAAGTCAGGCCAAAAAAGTTGATG
CAGCTGCCAATCAAGGCAAAAAATCAGAGATGGCGCCTGCTCAAGGTCAGAAGGCAAGCATGGTGCAGTC
TCAGGAAGCGCCAAAGCAGGATGCTCCTGCCAAAAAGAAATCAGGCAGCCGCAAGAAAGGTGAGCCAGTC
TGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216072 representing NM_133626
Red=Cloning site Green=Tags(s)

MDIYDTQTLGVVVFGGFMVVSAIGIFLVSTFSMKETSYEEALANQRKEMAKTHHQKGEKKKKEKTVEKKG
KTKKKEEKPNGKIPEHDLDPNVTIILKEPVRVSAVAVAPTSVHSSVGHTPIATVPAMPQEKLASSPKDRK
KKEKKVAKVEPAVSSIVNSIQVLASKSAILEATPKEVPMVAVPPVGSKASSPATSSQGKKGQGAQNQAKK
GEGAQNQGKKGEGAQNQAKKGEGAQNQAKKGEGAQNQGKKGEGAQNQAKKGEGGQNQAKKGEGAQNQGKK
GEGAQNQGKKGEGAQNQAKKGEGAQNQAKKGEGAQNQGKKGEGAQNQSKKGEGAQNQAKKGEGGQNQAKK
GEGAQNQAKKGEGAQNQAKKGEGVQNQAKKGVEGAQNQGKKGEANQNQAKKGEGGQNQTKKGEGPQNQGK
KGEAAQKQDKKIEGAQNQGKKPEGTSNQGKKGEGAQNQGKKGEGAQNQGKKGEGAQNQGKKGEGAQNQGK
KGEGAQNQGKKGEGAQNQGKKGEGAQNQGKKGEGPQNQAKKGEGAQNQGKKGEGAQNQGKKGEGAQNQGK
KAEGVQSQSKKGEGTQNQGKKGDGNPNQGKKGEGASNQNRKTDTVANQGTKQEGVSNQVKKSEGSPNQGK
KAEGAPNQGKKKDGSPSQAKKVDAAANQGKKSEMAPAQGQKASMVQSQEAPKQDAPAKKKSGSRKKGEPV
C

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133626
ORF Size 2103 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133626.2, NP_598329.1
RefSeq Size 2706 bp
RefSeq ORF 2106 bp
Locus ID 81910
UniProt ID Q99PL5
Cytogenetics 2 G1
Summary Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133626" in other vectors (2)
SKU Description Size Price
MC220604 Rrbp1 (untagged) - Mouse ribosome binding protein 1 (Rrbp1), transcript variant 2, (10ug) 10 ug
$707.00
MR216072 Rrbp1 (Myc-DDK-tagged) - Mouse ribosome binding protein 1 (Rrbp1), transcript variant 2. Note: ORF is codon optimized 10 ug
$589.00 $642.00

file-alt Citations

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