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Ndrg2 (NM_013864) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216067
Ndrg2 (tGFP-tagged) - Mouse N-myc downstream regulated gene 2 (Ndrg2) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $703.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ndrg2
Synonyms AI182517; AU040374; Ndr2; SYLD
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216067 representing NM_013864
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAACTTCAGGAGGTGCAGATCACCGAGGAGAAGCCGCTGTTGCCAGGACAAACACCCGAGACTG
CCAAGGAGGCCGAGTTAGCTGCCCGCATCCTCCTGGACCAGGGACAGACTCACTCTGTGGAGACACCTTA
TGGCTCGGTCACTTTTACTGTGTATGGCACCCCCAAACCCAAACGCCCGGCAATATTCACCTACCATGAT
GTAGGCCTCAACTATAAGTCTTGCTTCCAGCCACTGTTTCGGTTCGGGGATATGCAAGAGATCATACAGA
ACTTCGTGCGGGTTCATGTGGATGCCCCTGGCATGGAAGAGGGGGCGCCTGTGTTTCCTCTGGGATATCA
GTACCCATCTCTGGACCAGCTTGCTGACATGATTCCTTGCATCCTGCAGTACCTAAATTTCTCTACAATA
ATTGGAGTTGGTGTTGGAGCCGGAGCCTACATTCTGTCGCGATATGCTCTGAACCACCCGGACACAGTTG
AAGGTCTTGTTCTCATCAACATTGATCCCAATGCCAAGGGCTGGATGGACTGGGCAGCCCACAAGTTAAC
TGGCCTTACGTCTTCCATTCCGGACATGATTCTTGGACATCTTTTCAGCCAGGAAGAGCTTTCTGGAAAT
TCTGAATTGATACAAAAGTACAGAGGTATCATCCAACACGCACCCAACCTGGAGAACATTGAACTCTACT
GGAACAGCTACAACAACCGCCGAGACCTGAACTTTGAGCGAGGTGGTGAGACGACCCTCAAGTGCCCTGT
GATGCTGGTGGTTGGAGACCAAGCACCTCATGAAGATGCCGTGGTGGAATGTAACTCAAAGCTGGACCCC
ACACAGACCTCGTTCCTCAAGATGGCCGACTCTGGAGGTCAGCCACAGCTGACTCAGCCCGGCAAGCTGA
CAGAGGCTTTCAAGTACTTCCTGCAAGGCATGGGCTACATGGCCTCTTCCTGCATGACTCGCCTATCTCG
GTCTCGCACAGCATCTCTGACCAGTGCAGCATCCATCGATGGCAGTCGGTCCCGATCCCGCACCCTGTCG
CAGAGTAGCGAGTCTGGGACTCTCCCTTCTGGACCCCCAGGGCACACCATGGAAGTCTCCTGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216067 representing NM_013864
Red=Cloning site Green=Tags(s)

MAELQEVQITEEKPLLPGQTPETAKEAELAARILLDQGQTHSVETPYGSVTFTVYGTPKPKRPAIFTYHD
VGLNYKSCFQPLFRFGDMQEIIQNFVRVHVDAPGMEEGAPVFPLGYQYPSLDQLADMIPCILQYLNFSTI
IGVGVGAGAYILSRYALNHPDTVEGLVLINIDPNAKGWMDWAAHKLTGLTSSIPDMILGHLFSQEELSGN
SELIQKYRGIIQHAPNLENIELYWNSYNNRRDLNFERGGETTLKCPVMLVVGDQAPHEDAVVECNSKLDP
TQTSFLKMADSGGQPQLTQPGKLTEAFKYFLQGMGYMASSCMTRLSRSRTASLTSAASIDGSRSRSRTLS
QSSESGTLPSGPPGHTMEVSC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013864
ORF Size 1113 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013864.1
RefSeq Size 2157 bp
RefSeq ORF 1116 bp
Locus ID 29811
UniProt ID Q9QYG0
Cytogenetics 14 C2
Summary Contributes to the regulation of the Wnt signaling pathway. Down-regulates CTNNB1-mediated transcriptional activation of target genes, such as CCND1, and may thereby act as tumor suppressor. May be involved in dendritic cell and neuron differentiation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013864" in other vectors (4)
SKU Description Size Price
MC209811 Ndrg2 (untagged) - Mouse N-myc downstream regulated gene 2 (Ndrg2), transcript variant 1, (10ug) 10 ug
$457.00
MR216067 Ndrg2 (Myc-DDK-tagged) - Mouse N-myc downstream regulated gene 2 (Ndrg2), transcript variant 1 10 ug
$503.00
MR216067L3 Lenti ORF clone of Ndrg2 (Myc-DDK-tagged) - Mouse N-myc downstream regulated gene 2 (Ndrg2), transcript variant 1 10 ug
$803.00
MR216067L4 Lenti ORF clone of Ndrg2 (mGFP-tagged) - Mouse N-myc downstream regulated gene 2 (Ndrg2), transcript variant 1 10 ug
$803.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.