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Trcg1 (NM_001014398) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216058
Trcg1 (tGFP-tagged) - Mouse taste receptor protein 1 (Trcg1), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $955.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trcg1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216058 representing NM_001014398
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACAAGCAGTGGTTTCCTGCAGCTGGAATTCTCTTGGCTGCCCTCCTAGTAGTCTCTGCTTCTACCC
TGACCCTTCTCTCTACTAATGAAGACCCTGAGCAGTTTCCCTCAGCCCCTGGCACATCAGCTCAGCAAAG
TAGCCGCATTCTACTGGGCATCCTGACAGACGTCACTGGTGGTATCAACTCAGTTGAGAGGGAACCGGAG
GCCCTGGGGAGGAGGGCAGGAGGCCTCTCTACAGAAGGAGCTGGGGGTCAGGAGTCTCCCTCAATGCCTG
GCCCCTCAGGCAGGGTCATACCTGAACCAATTCCCTCAGCCCTGACCACATCTGCATCCGACATGGCCTC
TCAGCCAGTGTCCTCTGGGGCTGACCCTATAGAGGAAATCATGGCTCTTGGAACTCTAGAGACAATTACG
ATGTCATCACCACAGCCTTCTCCCAGACATGAATCTGAGCAGAAGTTCGACAAGGTCTTCAGGTTGTGCG
GGACAGTTCCTGCCTCTGAAACACTGGAGAAAAATGTTGCATCTAAACCAGCTTGGCATTTAATTAAAGC
TCCCCCTCCCCCCATATTCTCTAGATCTCCACACCTGTTATGGTGTACCCCCAATTCCACTGTCTACATA
CCAGTGCCTGCATGGAGGGATGGCCACTCCAGGCCAGAGGCATCCTCATCTGTGCCACTAGCTCCAAGTA
CCTCCTTAGGACTGCCTATCTTTCCATGGATGCCTAACATACTGAAAGCTACAGAGTCCCTGTTGCCTGC
ATCTCCTGGAAGATCAGGGCTGGACCTCACCTCCCAAGTGGGCTCCAGAGCATCTGAAAACACCGTGGCT
TTGGACACAGGCCCAGTATCCCGTGGTACTTCTGTGCTGCTGTTGCCCTCAGCAACATCCCCTTCACAGG
CATCCTCCCTCCACTCCCCTCGCCCCTCATCAGCATCACCCCTGTCTGCATCCCCATCACCTGCAGCTTT
GTCCTTCTTCCCATCACCAGCCTCAATCCTGGTCCCGGTTTCCTCTGTCACCACAGGAGCCAGTGACTCT
CCTAAAGCTCCTGTGTCTGTCATTGCACCCTCAACTACAGACTCTTTCATTAAAACCTCAAACCTCGGAC
CCCAGATAGCTCTACAACCTAGTCACCCTGGGCTATGGCTTCCCACCAGCCCAATCCACATGCCCACGCT
CTCCCTCCAACATTTCTCTAGCCCTCCCTCTACCGCACATAGCTCTGGCTTCACAGAGTCATCTGTACAT
GCTGATCCTACCCTGGCCTCTACCCTCCCTCACCCTGGCCAGGATATGTCTTTGCAGGACTTGAGTTTCT
CCACTGGAGGACGTAGTCATACGACCCACTCTGTGACCTTTAGGATCAACAGCAATCGCTTCACAAAAGC
TGTCTGGAACCTGGTACCCTTGGAGCGCTGGCTGCTGAACAGGCTTATCTGCTACCAGCTCCGGTTCATC
TACCAGGAGGCCTTCCCCAACTTCAGGAATGTCAGCGCCCTGCTGTTTCGGCCTGGCTGTCCAGAGGTGA
AAGCCTCCCTCATTTTTGGTCCTCCGGATCCCTCGTCCATAGAAATCCTCTGGACTTTGTACCGCAAAGT
GAAGTCCTCCAGATGGTCGCTTGGGTACCTGTCCCTGGCCGACCATGGCCTTTCCTCTGATGGGTACAGC
ATGACTGACCTTACCCAGGAAATCATCAACATTAGCTTCACACTCATGAGGCCCTTCCTGCCCCAGCTGC
TTCTGCCTAGTTCTCAGTCTTGTATCCTGCTGGAAAAGCAGACCATCCAGCTGGTCACCCACGAAGTGTC
AAGATTCTACAAGGCTGAGCTCCAGAGCCAGCCCCTGCTCCTATTCAGCAACGTGAAGGAGTGGGTGAGC
GTTTACATGGAATACAAATTCAAGAGCCCCATCCCCATCCGTCTCCAAGGCCTGGCCAGTCACCTGGCCC
ATCATATAACAGATCCCACCCTTCAGAAATCCAGCATAATGGCCAATGGGGAGAAAGCAGATCTGGTGTT
TTATGAGATGTGGCTCTTGATCCTGGGTCACCCCTTCACCAAGACCTTGGAGAACAAGACTAGTTCTGAG
TGCCAGGAGCTTCGTGGACTGCTGACGAGACAGCTAACCTCAGTCCTCCAGCCTTTGAAGAACTTTGGTC
AAGTGGTGGTGGAGGAATTCCACCAGGAACCACTGACTGCCAGGGTACAAACGGCCTTCTTTGGGGCTGT
GCCAGCCCAGGCCATCATTCAAGACACCGTGCTCCAAGCCCTGGGCTCCCTGCAGGAAACTGAGGGTCTG
CAGTTAGAGATGCTCCTCCCAGTCCTTGGCACCCCCAGCTCCAGAGCCTCGAGAGGCCCCAGGGGCGGGG
CCATGCTGAACCTCCAGCGCTTCACTTCTCTCTTTGTCCTGGTGGCTCTTTGTACGGCTCCTCCCTTCAT
CAATAAGCAAGCCCTATACCTCTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216058 representing NM_001014398
Red=Cloning site Green=Tags(s)

MDKQWFPAAGILLAALLVVSASTLTLLSTNEDPEQFPSAPGTSAQQSSRILLGILTDVTGGINSVEREPE
ALGRRAGGLSTEGAGGQESPSMPGPSGRVIPEPIPSALTTSASDMASQPVSSGADPIEEIMALGTLETIT
MSSPQPSPRHESEQKFDKVFRLCGTVPASETLEKNVASKPAWHLIKAPPPPIFSRSPHLLWCTPNSTVYI
PVPAWRDGHSRPEASSSVPLAPSTSLGLPIFPWMPNILKATESLLPASPGRSGLDLTSQVGSRASENTVA
LDTGPVSRGTSVLLLPSATSPSQASSLHSPRPSSASPLSASPSPAALSFFPSPASILVPVSSVTTGASDS
PKAPVSVIAPSTTDSFIKTSNLGPQIALQPSHPGLWLPTSPIHMPTLSLQHFSSPPSTAHSSGFTESSVH
ADPTLASTLPHPGQDMSLQDLSFSTGGRSHTTHSVTFRINSNRFTKAVWNLVPLERWLLNRLICYQLRFI
YQEAFPNFRNVSALLFRPGCPEVKASLIFGPPDPSSIEILWTLYRKVKSSRWSLGYLSLADHGLSSDGYS
MTDLTQEIINISFTLMRPFLPQLLLPSSQSCILLEKQTIQLVTHEVSRFYKAELQSQPLLLFSNVKEWVS
VYMEYKFKSPIPIRLQGLASHLAHHITDPTLQKSSIMANGEKADLVFYEMWLLILGHPFTKTLENKTSSE
CQELRGLLTRQLTSVLQPLKNFGQVVVEEFHQEPLTARVQTAFFGAVPAQAIIQDTVLQALGSLQETEGL
QLEMLLPVLGTPSSRASRGPRGGAMLNLQRFTSLFVLVALCTAPPFINKQALYLS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001014398
ORF Size 2475 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001014398.2, NP_001014420.2
RefSeq Size 2727 bp
RefSeq ORF 2478 bp
Locus ID 541610
UniProt ID Q58Y74
Cytogenetics 9 B
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Documents
Resources
"NM_001014398" in other vectors (4)
SKU Description Size Price
MC221918 Trcg1 (untagged) - Mouse taste receptor cell gene 1 (Trcg1), (10ug) 10 ug
$832.00
MR216058 Trcg1 (Myc-DDK-tagged) - Mouse taste receptor cell gene 1 (Trcg1) 10 ug
$589.00 $755.00
MR216058L3 Lenti ORF clone of Trcg1 (Myc-DDK-tagged) - Mouse taste receptor cell gene 1 (Trcg1) 10 ug
$1,055.00
MR216058L4 Lenti ORF clone of Trcg1 (mGFP-tagged) - Mouse taste receptor cell gene 1 (Trcg1) 10 ug
$1,055.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.