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Rnf19a (NM_013923) Mouse Tagged ORF Clone

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SKU
MG216055
Rnf19a (tGFP-tagged) - Mouse ring finger protein 19A (Rnf19a), (10ug)
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ORF Plasmid:
tGFP DDK
  $969.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rnf19a
Synonyms AA032313; Dorfin; Rnf19; Ubce7ip2; UIP117; XYbp
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216055 representing NM_013923
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAAGAACAAGAAATAAGTTTTATTTTTAAGTATAATGAAGGGCTGTGTATGAACATTGACTCTGACT
CAATTCTGATGAGCATTTTAGACATGAGTTTACATCAGCAAATGGGCTCAGATAGAGATCTTCAGTCTTC
TACTTCCTCCGTGAGCTTGCCTTCTGTCAAAAAGGCACCCAAACAGAGAAGGATTTCAATAGGCTCTCTC
TTTCGGAGGAAGAAGGACAGCAAACGAAAATCAAGGGAGCTCAATGGTGGAGTGGATGGAATTGCAAGTA
TTGAGAGTATACATTCTGAAATGTGTGCTGATAAGAACTCCATTTTCTCTACAAATACCTCTTCTGACAA
TGGATTGACTTCCATCAGCAAACAAATTGGAGACTTTATAGAATGTCCTTTGTGCCTTTTACGGCATTCT
AAAGACAGATTTCCTGATATAATGACATGCCACCATAGATCTTGTGTGGATTGCTTACGACAATACCTAA
GGATAGAAATCTCTGAAAGTAGAGTTAATATCAGTTGTCCAGAATGCACTGAACGGTTTAATCCTCATGA
CATTCGCTTGATATTAAGTGATGATGTCTTAATGGAAAAATATGAAGAATTTATGCTTCGACGGTGGCTT
GTTGCAGATCCTGACTGTAGGTGGTGTCCGGCTCCAGACTGTGGATACGCTGTGATAGCATTTGGATGTG
CCAGCTGTCCAAAACTAACTTGTGGGCGAGAAGGCTGTGGAACAGAGTTTTGCTACCACTGTAAACAGAT
CTGGCATCCAAACCAGACCTGTGATGCTGCTCGACAGGAAAGAGCCCAAAGTTTACGCTTAAGAACTATA
CGTTCTTCATCTATTAGTTACAGCCAGGAATCTGGAGCAGCAGCTGATGATATAAAGCCATGTCCACGAT
GTGCTGCTTATATAATAAAGATGAATGATGGGAGTTGCAATCATATGACATGTGCTGTCTGTGGTTGTGA
GTTTTGTTGGCTATGTATGAAAGAAATCTCCGATTTACATTATCTAAGTCCCTCAGGTTGTACTTTTTGG
GGGAAGAAACCCTGGAGCCGAAAGAAGAAAATCCTGTGGCAGCTGGGAACCCTTGTTGGTGCTCCCGTAG
GGATTGCTCTCATAGCTGGCATTGCCATTCCCGCCATGATCATTGGCATTCCTGTGTACGTGGGCCGCAA
GATTCATAATCGCTATGAAGGCAAAGATGTTTCAAAACATAAGCGGAATCTGGCCATAGCAGGCGGTGTC
ACCCTGTCTGTAATCGTGTCTCCGGTGGTAGCTGCAGTGACTGTAGGCATTGGTGTTCCCATCATGCTAG
CTTATGTCTATGGTGTGGTCCCAATTTCTCTCTGCCGAAGTGGGGGTTGTGGTGTCTCTGCGGGCAATGG
GAAAGGAGTGAGGATTGAGTTTGATGATGAGAATGACATAAACGTCGGTGGGACTAACGCAGCCATAGAT
ACAACATCAGTAGCAGAAGCAAGGCACAACCCTAGCATAGGAGAGGGAAGTGTCGGCGGCCTGACTGGCA
GTTTGAGTGCAAGTGGAAGCCACATGGATCGAATAGGCACCATTAGAGACAACCTGAGTGAGACAGCGAG
CACCATGGCATTAGCTGGAGCTAGTATCACGGGCAGTCTGTCGGGAAGTGCCATGGTAAACTGTTTCAAC
AGGTTGGAAGTACAAGCAGACGTACAGAAAGAACGGTGTAGTCTCAGTGGAGAGTCTGGCACAGTGAGCT
TGGGAACAGTTAGTGATAACGCCAGCACCAAAGCAATGGCGGGATCCATTCTCAACTCCTACATCCCTCT
GGACAGAGAAGGGAACAGCATGGAAGTGCAAGTGGATATTGAGTCAAAGCCATTCAAGTTCCGACACAAC
AGTGGAAGCAGCAGTGTGGATGACAGTGGTGCTACCCGAGGCCACACAGGCGGGGCGTCCAGTGGCTTGC
CCGAAGGTAAATCCAGTGCTACCAAGTGGTCCAAGGAAGCAACAGGAGGAAAAAAGTCAAAAAGTGGTAA
ACTGAGGAAAAAGGGTAACATGAAGATCAATGAGACCAGAGAGGACATGGATGCACAGTTGTTAGAGCAA
CAAAGCACGAACTCAAGTGAGTTTGAGGCGCCATCCCTCAGTGACAGCATGCCGTCTGTAGCAGATTCCC
ACTCCAGTCATTTTTCTGAATTTAGCTGCTCAGACTTAGAAAGCATGAGAACGTCCTGTAGTCACGGCTC
CAGTGATTGCCACGCCCGCTTCACAGCTGTGAACACCCTTCCTGAGGTAGAAAACGACCGACTGGAGAAC
TCACCACACCAGTGCAGCAGCGCTCTGCTTTCCAAAGCTGCTTCCTGTTCAGATGTCCCACAGCCCAGCC
ATGCTGCAGACGAGCATGGCACCAGCAGAAGTGGGGGGAAGCCTATGGTGGATTTGTGTTTTGGTGATGC
ACTGAGAGAGACAAATAATAACCACTCGCACCAGACAGCAGACCTGAAAGTTGCTGTCCAGACTGAAATT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216055 representing NM_013923
Red=Cloning site Green=Tags(s)

MQEQEISFIFKYNEGLCMNIDSDSILMSILDMSLHQQMGSDRDLQSSTSSVSLPSVKKAPKQRRISIGSL
FRRKKDSKRKSRELNGGVDGIASIESIHSEMCADKNSIFSTNTSSDNGLTSISKQIGDFIECPLCLLRHS
KDRFPDIMTCHHRSCVDCLRQYLRIEISESRVNISCPECTERFNPHDIRLILSDDVLMEKYEEFMLRRWL
VADPDCRWCPAPDCGYAVIAFGCASCPKLTCGREGCGTEFCYHCKQIWHPNQTCDAARQERAQSLRLRTI
RSSSISYSQESGAAADDIKPCPRCAAYIIKMNDGSCNHMTCAVCGCEFCWLCMKEISDLHYLSPSGCTFW
GKKPWSRKKKILWQLGTLVGAPVGIALIAGIAIPAMIIGIPVYVGRKIHNRYEGKDVSKHKRNLAIAGGV
TLSVIVSPVVAAVTVGIGVPIMLAYVYGVVPISLCRSGGCGVSAGNGKGVRIEFDDENDINVGGTNAAID
TTSVAEARHNPSIGEGSVGGLTGSLSASGSHMDRIGTIRDNLSETASTMALAGASITGSLSGSAMVNCFN
RLEVQADVQKERCSLSGESGTVSLGTVSDNASTKAMAGSILNSYIPLDREGNSMEVQVDIESKPFKFRHN
SGSSSVDDSGATRGHTGGASSGLPEGKSSATKWSKEATGGKKSKSGKLRKKGNMKINETREDMDAQLLEQ
QSTNSSEFEAPSLSDSMPSVADSHSSHFSEFSCSDLESMRTSCSHGSSDCHARFTAVNTLPEVENDRLEN
SPHQCSSALLSKAASCSDVPQPSHAADEHGTSRSGGKPMVDLCFGDALRETNNNHSHQTADLKVAVQTEI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013923
ORF Size 2520 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013923.2, NP_038951.1
RefSeq Size 4276 bp
RefSeq ORF 2523 bp
Locus ID 30945
UniProt ID P50636
Cytogenetics 15 B3.1
Summary E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013923" in other vectors (4)
SKU Description Size Price
MC222004 Rnf19a (untagged) - Mouse ring finger protein 19A (Rnf19a), (10ug) 10 ug
$847.00
MR216055 Rnf19a (Myc-DDK-tagged) - Mouse ring finger protein 19A (Rnf19a) 10 ug
$589.00 $769.00
MR216055L3 Lenti ORF clone of Rnf19a (Myc-DDK-tagged) - Mouse ring finger protein 19A (Rnf19a) 10 ug
$1,069.00
MR216055L4 Lenti ORF clone of Rnf19a (mGFP-tagged) - Mouse ring finger protein 19A (Rnf19a) 10 ug
$1,069.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.