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Arhgap10 (NM_030113) Mouse Tagged ORF Clone

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SKU
MG216047
Arhgap10 (tGFP-tagged) - Mouse Rho GTPase activating protein 10 (Arhgap10), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $920.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Arhgap10
Synonyms A930033B01Rik; PSGAP-m; PSGAP-s
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216047 representing NM_030113
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCTGCAGCCCCTGGAGTTTAGCGACTGCTACCTCGACAGCCCGTGGTTCCGGGAGAGGATCCGCG
CGCACGAAGCGGAGCTCGAGAGGACCAACAAGTTCATCAAAGAGCTCATCAAAGACGGCAAGAACCTCAT
CTCTGCCACCAAAAGTCTGTCAGCTGCCCAGAGGAAGTTCGCTCACTCGCTCAGAGACTTCAAGTTCGAG
TTTATCGGCGATGCTGAGACTGACGATGAACGGTGCATAGATGCCTCCTTACGGGAATTTTCTAACTTTC
TGAAGAATCTGGAAGAACAGAGAGAAATCATGGCATTAAGTGTGACTGAAACCCTGATTAAACCCTTGGA
GAAATTCAGAAAAGAGCAGCTTGGAGCTGTAAAGGAAGAAAAAAAGAAGTTTGACAAAGAGACGGAAAAG
AATTACAGTCTCATCGACAAGCACTTGACGCTGTCTGCCAGGAAGAAGGACTCACACTTGCAGGAGGCCG
ACCTCCAAGTGGAGCAGAACCGGCAGCATTTCTACGAGCTCTCGCTTGAATATGTGTGCAAGCTCCAGGA
AATCCAAGAAAGAAAGAAGTTCGAGTTTGTGGAGCCTATGCTGTCCTTCTTTCAGGGAATGTTCACTTTC
TATCATCAAGGCCACGAGCTTTCCAAAGACTTCAATCACTACAAGATGGAACTACAAATCAACATTCAGA
ATACACGGAATCGATTTGAGGGAACCAGATCAGAAGTGGAGGAACTTATGAATAAAATTAGACAAAATCC
CAAGGACCAGAAGCGAGCCAGCCAGTTCACAGCAGAAGGCTACCTGTACGTCCAGGAGAAAAGGCCTGCT
CCATTTGGGTCCAGTTGGGTCAAACACTACTGCATGTACCGGAAAACAGCCAAGAAATTCAACATGATTC
CATTTGAGCACAGATCTGGGGGGAAACTGGGGGATGGCGAGGCTTTCTTTTTGAAAGAATGCACCAAAAG
GCACATGGACTCCACGGACAGAAGGTTTTGCTTTGACATCGAAGCTGCTGACAGGCCTGGCGTCCCCTTG
ACCGTGCAGGCGTTTTCAGAAGAAGAGAGGAAGCAGTGGTTGGAAGCTCTGGGTGGAAAAGAAGCTCTGT
TCCACACGTTTAACAGAGCCATTGTCCCGAGACCGGAAGGAGGTGCACAGCTGGATAAGATGGGATTCAC
AATTCTCAGAAAATGCATCAGCGCCGTTGAAACCCGAGGTATAAATGACCAAGGACTGTACAGAGTTGTG
GGGGTGAGTTCAAAGGTCCAGAGACTTCTGAGTATGTTGATGGATGTAAAAATGTGCAATGAGCTGGACC
TGGAGAATTCTGCAGATTGGGAAGTGAAGACAGTAACAAGTGCCTTGAAGCAGTATCTGAGGAGTCTTCC
AGAGCCTCTCATGACCTATGAGTTACACAGAGACTTCATTGTTCCAGCCAAGAGCGGCAGCCCGGAATCC
CGAGTTAATGCCATCCACTTCTTGGTACACAAACTGCCAGAGAAGAATAAGGAGATGTTGGATATCTTGG
TGAAGCATCTAACCAACGTGTCAAGTCACTCGAAGCAGAATCTAATGACAGTGGCGAATTTAGGGGTGGT
GTTTGGACCAACTCTGATGAGACCACAGGAAGAGACTGTTGCCGCCATCATGGATTTGAAGTTTCAGAAT
ATCGTTGTAGAAATCTTAATTGAAAACCATGAAAAGATTTTTCGAACCTCGCCTGATACCACATTTGCTG
AGCCCACATGCCTGTCAGCATCACCCCCAAACGCTCCACCACGACAGTCCAAGAGACAAGGGCAGAGGAC
GAAGAGACCAGTGGCCGTGTACAACCTTTGTCTTGAGCTGGAGGAGGGTGACAGTCCCAGCCCTCTCAAG
GAGGACCCCCCCAGCAGCAGTCAGGACTCTCTCTCCACCCCATCTCCCACAACGTCAGCTGCCCATGGGC
CTCCTGGACTAGACGGAAACCACCTAGCAGCAGACGGAGGGAGCTGTGGAGATGCCACCGCCACCACGCC
AAGCCAGACCCGGCCATCCATGGTGCAGTGGCTAAATATGCAGTCTCCAACCACACCAAGCTCCAACCCA
GCTGGAACACCTCCTTCACCCAGAATGTCACCGTTCCCCCTCTCTCCTGCTGCCTCCATTGTAGACAAGC
TGCCTGAGTGCGTTATCAATCGAAAGGCTCGGGCAGTGTACCCGTGTGAAGCAGAACACAGCTCGGAATT
GTCATTTGAAATAGGAGCCATTTTTGAGGATGTTCAGACCTCAAGGGAACCCGGATGGCTGGAAGGGACC
CTGAACGGCAAGCGAGGGCTGATTCCACAGAATTATGTCAAGCTCCTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216047 representing NM_030113
Red=Cloning site Green=Tags(s)

MGLQPLEFSDCYLDSPWFRERIRAHEAELERTNKFIKELIKDGKNLISATKSLSAAQRKFAHSLRDFKFE
FIGDAETDDERCIDASLREFSNFLKNLEEQREIMALSVTETLIKPLEKFRKEQLGAVKEEKKKFDKETEK
NYSLIDKHLTLSARKKDSHLQEADLQVEQNRQHFYELSLEYVCKLQEIQERKKFEFVEPMLSFFQGMFTF
YHQGHELSKDFNHYKMELQINIQNTRNRFEGTRSEVEELMNKIRQNPKDQKRASQFTAEGYLYVQEKRPA
PFGSSWVKHYCMYRKTAKKFNMIPFEHRSGGKLGDGEAFFLKECTKRHMDSTDRRFCFDIEAADRPGVPL
TVQAFSEEERKQWLEALGGKEALFHTFNRAIVPRPEGGAQLDKMGFTILRKCISAVETRGINDQGLYRVV
GVSSKVQRLLSMLMDVKMCNELDLENSADWEVKTVTSALKQYLRSLPEPLMTYELHRDFIVPAKSGSPES
RVNAIHFLVHKLPEKNKEMLDILVKHLTNVSSHSKQNLMTVANLGVVFGPTLMRPQEETVAAIMDLKFQN
IVVEILIENHEKIFRTSPDTTFAEPTCLSASPPNAPPRQSKRQGQRTKRPVAVYNLCLELEEGDSPSPLK
EDPPSSSQDSLSTPSPTTSAAHGPPGLDGNHLAADGGSCGDATATTPSQTRPSMVQWLNMQSPTTPSSNP
AGTPPSPRMSPFPLSPAASIVDKLPECVINRKARAVYPCEAEHSSELSFEIGAIFEDVQTSREPGWLEGT
LNGKRGLIPQNYVKLL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030113
ORF Size 2358 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030113.2, NP_084389.2
RefSeq Size 3038 bp
RefSeq ORF 2361 bp
Locus ID 78514
UniProt ID Q6Y5D8
Cytogenetics 8 C1
Summary GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_030113" in other vectors (4)
SKU Description Size Price
MC221582 Arhgap10 (untagged) - Mouse Rho GTPase activating protein 10 (Arhgap10), (10ug) 10 ug
$793.00
MR216047 Arhgap10 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 10 (Arhgap10) 10 ug
$589.00 $720.00
MR216047L3 Lenti ORF clone of Arhgap10 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 10 (Arhgap10) 10 ug
$1,020.00
MR216047L4 Lenti ORF clone of Arhgap10 (mGFP-tagged) - Mouse Rho GTPase activating protein 10 (Arhgap10) 10 ug
$1,020.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.