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Nlrp4f (NM_175290) Mouse Tagged ORF Clone

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SKU
MG216040
Nlrp4f (tGFP-tagged) - Mouse NLR family pyrin domain containing 4F (Nlrp4f), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,058.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nlrp4f
Synonyms C86045; C330026N02Rik; Nalp-kappa; Nalp4f
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216040 representing NM_175290
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCTTTCATTTCAGATTTTGGCCTTATATGGTATTTGAGAGAACTAAATAAGAAGGAATTCATGA
AGTTTAAGGACTTTCTCATACAGGAGATTCTGGAATTGAAACTGAAACAGGTATCTTCGACCAAAGTGAA
GAAAGCATCTCGGGAAGATCTTGCCAACTTACTTCTGAAATGTGGGGAGAATCAAGCCTGGGATATGACC
TTCAGAATCCTCCAGAAGATCAATAGGAAAGATCTCACTGAGAGGGCAACAGGAGCGATTGTTGGAAACC
CAAATTTATATCGAGATCATTTGAAGAAAAAACTGACCCATGATTGTCCCAAAAAATTTAACGTCCGTAT
TCAAGATTTCATTAAAGAGACTTTCATTCAAAATGACTATGATGCTTTTGAGAACCTTCTCATCTCAAAG
GGAACTGAAAGGAAGCCACACATGGTGTTCCTGAAAGGCATGGCTGGAGTTGGCAAGACACTGATGTTGA
AAAATTTAATGCTGGCCTGGTCAAAAGGCCTGGTGTTTCAAAACAAATTCTCTTACGCTTTCTACTTCTG
CTGTCAAGATGTGAAGCAGTTGAAGACAGCCAGCCTTGCTGAACTTATCTCCAGAGAGTGGCCCAGCCCC
TCGGCTCCTATAGAGGAGATCCTATCCCAACCCGAGAAACTCTTATTTATCATTGACAGCTTGGAAGGGA
TGGAATGGGATTTAACCAAACAGGAATCAGAGCTGTGTGATGACTGCATGGAGAAGCAGCCAGTGAGTAC
ACTGCTGAGCAGTTTGCTCAGGAGGAAGATGCTCCCTGAATCCTCTCTCCTCCTCTCCACTACCCCAGAG
ACTTTTGAGAAAATGGAGGACAGGATTCAGTGCACAGATGTGAAGACAGCAACTGCATTTGATGAGAGGA
GTATGAAGATATATTTCCACAGATTGTTCCAAGATAGGAAGAGAGCCCAGGAGGCCTTCAGTTTGGTGAG
AGAAAACAAGCAGCTGTTCACTATATGTCAGGTCCCTCTGCTCTGCTGGATGGTGGCTACTTGTCTAAAA
GAGGAGATAGAGAAGGGAGGAGACCCAGTCTCCCTCTGCCGACGTACCACCTCCCTATATACCACTCACA
TCTTCAGTTTGTTCATTCCCCAAAGTGCCCAGTATCCAAGTAAGAAAAGCCAAGACCAGCTGCAGGGCTT
ATGTTCTCTAGCTGCTGAGGGCATGTGGACTGACACATTTGTGTTTGGCAAGGAGGCTCTCAGGAGAAAT
GGGATCTTTGACTCTGACATCCCCACACTTTTGGACATTGGAATGCTTGGAAAGATCAGAGAATTTGAGA
ATTCTTACATATTCCTCCACCCATCTGTTCAGGAGGTCTGTGCTGCCATCTTTTATATGCTAAAGAGGCA
TGTGGAACACCCTAGCCAGGATGTTAAAAATATAGAGACAGTCTTGTTTATGTTTTTAAAGAAAGTAAAA
ACACAGTGGATTTTTTTGGGCTGTTTCATCTTTGGTCTTTTACAGAAATCAGAACAAGAAAAGCTAGGGG
TATTTTTTGGCCACCGGTTGTCCAAGAACATACACCATAAGTTATATCAGTGCCTGGAAACCTTAAGTGG
CAATGCAGAGCTTCAAGAACAAATAGACGGCATGAGATTGTTTTCCTGTCTGTTTGAGATGGAAGATGAA
GCCTTCCTAGTAAAAGCAATGAATTGTATGCAACAGATTAACTTTGTGGCTAAGAATTATTCTGATTTTA
TTGTTGCTGCTTATTGCTTGAAACACTGTTCTACACTGAAGAAACTATCCTTTTCAACTGAAAATGTCCT
GAATGAAGGAGACCAGAGTTATATGGAAGAGCTACTTATCTGTTGGAATAATATGTGCTCTGTGTTTGTA
AGGAGTAAAGACATTCAGGAACTCCGAATAAAAGACACTAATTTCAATGAGCCAGCCATTCGAGTTTTAT
ATGAATCTCTGAAGTACCCCAGCTTCACCCTTAACAAACTTGTGGCAAATAATGTGTCCTTTGGTGATAA
CCACGTGCTCTTTGAGTTGATTCAGAATTCCAGTTTGCAATACTTGGACCTCAGCTGCTCATTTCTGTCC
CACAATGAGGTGAAACTGTTGTGTGATATCTTGAACCAGGCAGAGTGCAACATAGAAAAACTTATGATAG
CACACTGTAAACTTTCACCTGATGACTGCAAGATCTTTGGCTCCATCCTGATGAGCAGCAAGTCGTTGAA
GGTTCTTAATTTGGCATCCAACAATTTGAATCAAGGAATATCCTCACTGTGCAAGGCTTTGTGCCACCCA
CACTGTACTCTGGAGTACTTAGTGCTGTCCAACTGTTCCCTCAGTGAGCAATGTTGGGACTACCTTTCTG
AAGTTCTTAGGCAGAACAAAACTCTGAGCCATCTAGACATCAGCTCCAATGACCTGAAGGATGAAGGACT
GAAGATTCTCTGTAGGTCTCTGATTCTCCCATACTGTGTCCTGGAGTCACTATGTTTGAGCTGTTGTGGA
ATCACCGAAAGGGGCTGCCAGGATCTGGCTGAAGTCCTGAAGAACAACCAGAACCTGAAGTACCTACATG
TTTCATACAATAAGCTAAAAGACACTGGTGTGATGCTGCTGTGTGATGCTATAAAACATCCTAACTGCCA
CTTAAAGGATCTACAGTTGGAAGCCTGTGAAATAACTGATGCCAGTAATGAGGAGCTTTGTTATGCTTTT
ATGCAGTGTGAGACCCTGCAGACGCTCAACCTCATGGGGAATGCCTTCGAAGTCAGTAGAATGGTTTTCT
TTCCCAGGTTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216040 representing NM_175290
Red=Cloning site Green=Tags(s)

MASFISDFGLIWYLRELNKKEFMKFKDFLIQEILELKLKQVSSTKVKKASREDLANLLLKCGENQAWDMT
FRILQKINRKDLTERATGAIVGNPNLYRDHLKKKLTHDCPKKFNVRIQDFIKETFIQNDYDAFENLLISK
GTERKPHMVFLKGMAGVGKTLMLKNLMLAWSKGLVFQNKFSYAFYFCCQDVKQLKTASLAELISREWPSP
SAPIEEILSQPEKLLFIIDSLEGMEWDLTKQESELCDDCMEKQPVSTLLSSLLRRKMLPESSLLLSTTPE
TFEKMEDRIQCTDVKTATAFDERSMKIYFHRLFQDRKRAQEAFSLVRENKQLFTICQVPLLCWMVATCLK
EEIEKGGDPVSLCRRTTSLYTTHIFSLFIPQSAQYPSKKSQDQLQGLCSLAAEGMWTDTFVFGKEALRRN
GIFDSDIPTLLDIGMLGKIREFENSYIFLHPSVQEVCAAIFYMLKRHVEHPSQDVKNIETVLFMFLKKVK
TQWIFLGCFIFGLLQKSEQEKLGVFFGHRLSKNIHHKLYQCLETLSGNAELQEQIDGMRLFSCLFEMEDE
AFLVKAMNCMQQINFVAKNYSDFIVAAYCLKHCSTLKKLSFSTENVLNEGDQSYMEELLICWNNMCSVFV
RSKDIQELRIKDTNFNEPAIRVLYESLKYPSFTLNKLVANNVSFGDNHVLFELIQNSSLQYLDLSCSFLS
HNEVKLLCDILNQAECNIEKLMIAHCKLSPDDCKIFGSILMSSKSLKVLNLASNNLNQGISSLCKALCHP
HCTLEYLVLSNCSLSEQCWDYLSEVLRQNKTLSHLDISSNDLKDEGLKILCRSLILPYCVLESLCLSCCG
ITERGCQDLAEVLKNNQNLKYLHVSYNKLKDTGVMLLCDAIKHPNCHLKDLQLEACEITDASNEELCYAF
MQCETLQTLNLMGNAFEVSRMVFFPRF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175290
ORF Size 2811 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175290.4, NP_780499.3
RefSeq Size 3527 bp
RefSeq ORF 2814 bp
Locus ID 97895
Cytogenetics 13 B3
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Documents
Resources
"NM_175290" in other vectors (4)
SKU Description Size Price
MC222745 Nlrp4f (untagged) - Mouse NLR family, pyrin domain containing 4F (Nlrp4f), (10ug) 10 ug
$945.00
MR216040 Nlrp4f (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 4F (Nlrp4f) 10 ug
$589.00 $858.00
MR216040L3 Lenti ORF clone of Nlrp4f (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 4F (Nlrp4f) 10 ug
$1,158.00
MR216040L4 Lenti ORF clone of Nlrp4f (mGFP-tagged) - Mouse NLR family, pyrin domain containing 4F (Nlrp4f) 10 ug
$1,158.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.