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Tnpo1 (NM_178716) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216038
Tnpo1 (tGFP-tagged) - Mouse transportin 1 (Tnpo1) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,022.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tnpo1
Synonyms AU021749; D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216038 representing NM_178716
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGTGGGACCGGCAAACCAAGATGGAGTATGAGTGGAAACCTGACGAGCAAGGGCTTCAGCAGATCC
TGCAGCTGCTCAAGGAGTCCCAGTCCCCAGACACCACCATCCAGAGGACCGTGCAACAAAAACTGGAACA
ACTCAATCAATATCCAGATTTTAACAACTACTTGATTTTTGTTCTTACAAAATTAAAATCTGAAGATGAG
CCCACACGGTCATTGAGCGGTCTGATCTTGAAGAACAATGTGAAAGCTCATTTTCAGAACTTCCCAAATG
GTGTGACAGACTTCATCAAGAGTGAATGTCTAAATAACATTGGGGACTCCTCTCCGTTGATCAGAGCCAC
TGTCGGTATTTTAATTACAACCATAGCCTCCAAGGGAGAGCTGCAGAATTGGCCTGATCTCTTACCAAAA
CTCTGTAGCCTGCTGGATTCCGAAGACTACAACACTTGTGAGGGAGCCTTCGGTGCCCTTCAGAAGATAT
GTGAGGACTCTGCAGAGATTTTAGACAGTGATGTCTTAGATCGCCCTCTCAACATCATGATCCCCAAGTT
TTTACAGTTTTTCAAGCACAGCAGTCCCAAAATAAGGTCTCATGCTGTTGCATGTGTCAATCAGTTCATC
ATCAGTCGGACCCAGGCGCTCATGCTGCACATCGATTCCTTCATTGAGAACCTCTTTGCACTGGCTGGCG
ACGAGGAAGCAGAGGTGCGGAAGAACGTGTGCCGGGCGCTTGTGATGTTGCTTGAAGTCCGGATGGATCG
CCTGCTTCCTCATATGCACAACATAGTCGAGTACATGCTGCAGAGAACCCAAGACCAAGATGAGAATGTA
GCTCTGGAGGCCTGTGAATTCTGGCTGACTTTGGCTGAACAGCCAATATGCAAAGATGTACTTGTAAGGC
ATCTACCAAAGTTGATTCCTGTGTTAGTGAATGGCATGAAGTACTCAGATATAGATATTATCCTGCTTAA
GGGTGATGTTGAGGAAGATGAGACCATCCCGGATAGTGAGCAGGATATACGGCCGCGGTTTCATCGCTCG
AGGACAGTGGCTCAGCAGCATGAGGAGGATGGGATTGAGGAGGAAGACGATGATGATGACGAAATTGATG
ATGATGACACGATTTCTGACTGGAACCTGAGAAAGTGTTCTGCTGCTGCTCTCGATGTTCTCGCAAATGT
TTATCGTGATGAGCTTTTGCCGCACATTCTGCCACTTTTGAAAGAATTGCTTTTCCATCATGAATGGGTT
GTGAAAGAATCTGGCATCTTGGTTTTAGGAGCAATTGCTGAAGGTTGTATGCAAGGCATGATTCCGTACC
TGCCCGAGCTCATTCCTCACCTTATTCAGTGCCTTTCTGATAAAAAGGCTCTTGTGCGTTCCATCACCTG
CTGGACTCTTAGCCGCTATGCACACTGGGTAGTCAGCCAGCCACCAGATACGTACCTGAAGCCATTAATG
ACAGAACTGCTGAAGCGTATCCTCGATAGCAACAAGAGAGTACAGGAAGCAGCTTGCAGTGCCTTCGCTA
CATTAGAAGAGGAGGCTTGTACAGAGCTCGTCCCTTACCTTGCTTATATACTCGATACCCTCGTCTTCGC
CTTCAGTAAATACCAGCATAAGAACCTGCTCATTCTGTACGACGCCATAGGGACACTGGCAGATTCAGTG
GGACATCATTTAAACAAGCCAGAATATATTCAGATGCTAATGCCTCCTTTGATCCAGAAATGGAACATGC
TGAAGGATGAAGACAAGGATCTTTTCCCTTTGCTTGAGTGTCTCTCGTCTGTTGCCACAGCCTTGCAGTC
TGGCTTCCTTCCATATTGTGAACCTGTATATCAGCGTTGCGTAAACCTAGTTCAGAAGACTCTGGCACAA
GCCATGCTAAACAATGCTCAACCAGAACAGTATGAAGCTCCAGATAAAGATTTTATGATTGTGGCTCTTG
ACTTACTCAGCGGCCTGGCTGAGGGCCTGGGAGGCAACATTGAGCAGCTGGTGGCCCGCAGTAACATCCT
GACGCTGATGTACCAGTGCATGCAGGATAAAATGCCCGAGGTTCGGCAGAGTTCTTTTGCATTGCTAGGT
GACCTGACTAAAGCGTGCTTCCAGCATGTTAAGCCTTGTATAGCTGATTTCATGCCAATATTGGGAACCA
ATCTAAATCCAGAGTTTATTTCAGTCTGCAACAATGCCACCTGGGCGATTGGGGAAATATCAATCCAAAT
GGGTATAGAGATGCAGCCCTACATCCCTATGGTGTTGCACCAGCTTGTGGAGATCATTAACAGACCCAAC
ACCCCAAAGACGCTGTTGGAGAACACAGCAATAACAATTGGTCGTCTTGGTTACGTTTGTCCTCAAGAGG
TGGCCCCCATGCTACAGCAGTTTATAAGACCCTGGTGTACCTCTCTGAGAAACATAAGAGACAATGAAGA
AAAAGATTCAGCATTCCGTGGGATTTGTACCATGATCAGTGTGAATCCCAGTGGCGTAATCCAAGATTTT
ATATTTTTTTGTGATGCTGTTGCATCATGGATTAACCCAAAAGATGATCTCAGAGACATGTTCTGTAAGA
TCCTTCATGGATTTAAAAACCAAGTTGGGGATGAAAATTGGAGGCGATTCTCTGACCAGTTTCCTCTTCC
CTTAAAAGAGCGTCTTGCAGCTTTTTATGGTGTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216038 representing NM_178716
Red=Cloning site Green=Tags(s)

MVWDRQTKMEYEWKPDEQGLQQILQLLKESQSPDTTIQRTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDE
PTRSLSGLILKNNVKAHFQNFPNGVTDFIKSECLNNIGDSSPLIRATVGILITTIASKGELQNWPDLLPK
LCSLLDSEDYNTCEGAFGALQKICEDSAEILDSDVLDRPLNIMIPKFLQFFKHSSPKIRSHAVACVNQFI
ISRTQALMLHIDSFIENLFALAGDEEAEVRKNVCRALVMLLEVRMDRLLPHMHNIVEYMLQRTQDQDENV
ALEACEFWLTLAEQPICKDVLVRHLPKLIPVLVNGMKYSDIDIILLKGDVEEDETIPDSEQDIRPRFHRS
RTVAQQHEEDGIEEEDDDDDEIDDDDTISDWNLRKCSAAALDVLANVYRDELLPHILPLLKELLFHHEWV
VKESGILVLGAIAEGCMQGMIPYLPELIPHLIQCLSDKKALVRSITCWTLSRYAHWVVSQPPDTYLKPLM
TELLKRILDSNKRVQEAACSAFATLEEEACTELVPYLAYILDTLVFAFSKYQHKNLLILYDAIGTLADSV
GHHLNKPEYIQMLMPPLIQKWNMLKDEDKDLFPLLECLSSVATALQSGFLPYCEPVYQRCVNLVQKTLAQ
AMLNNAQPEQYEAPDKDFMIVALDLLSGLAEGLGGNIEQLVARSNILTLMYQCMQDKMPEVRQSSFALLG
DLTKACFQHVKPCIADFMPILGTNLNPEFISVCNNATWAIGEISIQMGIEMQPYIPMVLHQLVEIINRPN
TPKTLLENTAITIGRLGYVCPQEVAPMLQQFIRPWCTSLRNIRDNEEKDSAFRGICTMISVNPSGVIQDF
IFFCDAVASWINPKDDLRDMFCKILHGFKNQVGDENWRRFSDQFPLPLKERLAAFYGV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178716
ORF Size 2694 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178716.3, NP_848831.2
RefSeq Size 5391 bp
RefSeq ORF 2697 bp
Locus ID 238799
UniProt ID Q8BFY9
Cytogenetics 13 52.24 cM
Summary Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed:11493596). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A (By similarity). In vitro, mediates nuclear import of SRP19 (By similarity). Mediates the import of histones H2A, H2B, H3 and H4 (PubMed:11493596). Mediates nuclear import of ADAR/ADAR1 in a RanGTP-dependent manner (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178716" in other vectors (3)
SKU Description Size Price
MR216038 Tnpo1 (Myc-DDK-tagged) - Mouse transportin 1 (Tnpo1), transcript variant 1 10 ug
$589.00 $822.00
MR216038L3 Lenti ORF clone of Tnpo1 (Myc-DDK-tagged) - Mouse transportin 1 (Tnpo1), transcript variant 1 10 ug
$1,122.00
MR216038L4 Lenti ORF clone of Tnpo1 (mGFP-tagged) - Mouse transportin 1 (Tnpo1), transcript variant 1 10 ug
$1,122.00

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