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Fbxl12 (NM_013911) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216031
Fbxl12 (tGFP-tagged) - Mouse F-box and leucine-rich repeat protein 12 (Fbxl12) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $530.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fbxl12
Synonyms 3110048D16Rik; Fbl12
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216031 representing NM_013911
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACTCTGTTTGACCTCCCAGACTTGGTGCTCTTGGAGATCTTCTCTTACCTCCCTGTCCGGGACC
GGATCCGCATCTCCAGGGTCTGTCACCGCTGGAAGAGGCTAGTGGATGACCGGTGGCTATGGCGACACGT
CGACCTGACGCTCTACACGATGCGGCCGAAAGTCATGTGGCACCTCCTGCGCCGGTACATGGCGTCCCGG
CTCTACTCGTTGCGCATGGGCGGCTACCTGTTTTCTGGCTCTCAGGCCCCACAGCTGTCCCCCGCCTTGA
TGAGGGCCCTGGGTCAGAAATGCCCCAATCTGAAGCGCCTGTGCCTGCACGTGGCTGACCTGAGCATGGT
GCCTATCACCAGCCTTCCTAGCACACTGAGGACCCTGGAGCTGCACAGCTGCGAAATCTCCATGATCTGG
TTGCAGAAAGAGCAGGACCCCACGGTGCTGCCTCTTCTGGAATGCATCGTGCTGGACCGAGTGCCGGCCT
TCCGCGATGAGCATCTGCAGGGCCTTACCCGATTCCGAGCCCTGCGCTCGCTGGTGCTGGGCGGCACCTA
CCGGGTCACTGAGACCGGGCTAGATGCCAGCCTGCAGGAGCTCAGCTACCTGCAAAGGCTTGAGGTGCTG
GGCTGCACCCTGTCAGCTGACAGCACGCTGCTGGCCATCAGCCGCCACCTTCGAGATGTGCGCAAGATTC
GGCTGACCGTTGGGGGCCTCTCAGCCCAGGGCCTGGTCTTCCTGGAGGGAATGCCTGTCCTGGAGAGTTT
GTGCTTCCAGGGTCCCCTTATCACCCCAGACATGCCCACACCCACTCAGATTGTGTCCTCCTGCCTCACC
ATGCCGAAGCTCAGAGTGCTTGAGGTGCAAGGGCTGGGCTGGGAGGGTCAGGAAGCAGAGAAAATCCTGT
GCAAGGGCCTGCCCCACTGCATAGTTATTGTTAGGGCTTGTCCCAAAGAATCCATGGATTGGTGGATG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216031 representing NM_013911
Red=Cloning site Green=Tags(s)

MATLFDLPDLVLLEIFSYLPVRDRIRISRVCHRWKRLVDDRWLWRHVDLTLYTMRPKVMWHLLRRYMASR
LYSLRMGGYLFSGSQAPQLSPALMRALGQKCPNLKRLCLHVADLSMVPITSLPSTLRTLELHSCEISMIW
LQKEQDPTVLPLLECIVLDRVPAFRDEHLQGLTRFRALRSLVLGGTYRVTETGLDASLQELSYLQRLEVL
GCTLSADSTLLAISRHLRDVRKIRLTVGGLSAQGLVFLEGMPVLESLCFQGPLITPDMPTPTQIVSSCLT
MPKLRVLEVQGLGWEGQEAEKILCKGLPHCIVIVRACPKESMDWWM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013911
ORF Size 978 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013911.2, NP_038939.2
RefSeq Size 2098 bp
RefSeq ORF 981 bp
Locus ID 30843
UniProt ID Q9EPX5
Cytogenetics 9 A3
Summary Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the polyubiquitination and proteasomal degradation of CAMK1 leading to disruption of cyclin D1/CDK4 complex assembly which results in G1 cell cycle arrest in lung epithelia (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013911" in other vectors (4)
SKU Description Size Price
MC202263 Fbxl12 (untagged) - Mouse F-box and leucine-rich repeat protein 12 (Fbxl12), transcript variant 1, (10ug) 10 ug
$300.00
MR216031 Fbxl12 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 12 (Fbxl12), transcript variant 1 10 ug
$330.00
MR216031L3 Lenti ORF clone of Fbxl12 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 12 (Fbxl12), transcript variant 1 10 ug
$630.00
MR216031L4 Lenti ORF clone of Fbxl12 (mGFP-tagged) - Mouse F-box and leucine-rich repeat protein 12 (Fbxl12), transcript variant 1 10 ug
$630.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.