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Pfkfb2 (NM_001162415) Mouse Tagged ORF Clone

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SKU
MG216027
Pfkfb2 (tGFP-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-26-biphosphatase 2 (Pfkfb2) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
  $703.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Pfkfb2 or NM_001162415"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pfkfb2
Synonyms 4930568D07Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216027 representing NM_001162415
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGAGAATAGTACATTTTCCCCAGAAGACTGCAACAGCAGCTATAAACCCCACGCCTCAAATCTGC
GAAGGGCAGGGAAGACATGCTCATGGGCTTCCTATATGACCAACTCCCCAACACTCATTGTTATGATTGG
CTTGCCAGCCCGGGGTAAAACTTATGTGTCTAAGAAACTAACACGCTACCTCAACTGGATTGGAGTACCC
ACCAAAGTGTTTAATCTTGGGGTATATCGACGTGAAGCAGTCAAGTCCTATCAGTCTTATGATTTCTTTC
GACATGACAATGAAGAGGCTATGAAGATCCGCAAACAGTGTGCTCTGGTGGCACTGGAAGATGTTAAAGC
CTATTTTACTGAAGAGAGTGGGCAGATTGCGGTGTTTGATGCCACCAATACCACTCGGGAGAGGAGGGAC
ATGATTTTGAACTTTGCCAAGCAGAATGCCTTCAAGGTATTTTTTGTAGAATCTGTCTGTGATGATCCTG
ATGTCATTGCTGCCAATATTCTGGAGGTAAAAGTGTCGAGCCCTGACTACCCCGAAAGGAATAGGGAGAA
TGTGATGGAGGACTTCCTGAAGAGAATTGAGTGCTACAAGGTCACTTACCAGCCCCTTGACCCAGACAAC
TATGATAAGGATCTTTCTTTCATAAAGGTGATAAATGTAGGCCAGAGATTCCTGGTCAACAGAGTTCAGG
ACTACATCCAGAGTAAGATTGTCTACTACCTGATGAATATCCACGTCCATCCTCGCACCATCTACCTTTG
CCGGCACGGCGAGAGCGAGTTCAACCTTTTGGGGAAGATTGGGGGTGACTCTGGCCTCTCCGTGCGAGGA
AAGCAGTTTGCTCATGCTCTGAAGAAGTTTCTGGAGGAACAGGAGATCCAGGACCTTAAAGTGTGGACGA
GCCAGTTGAAGAGGACAATTCAGACTGCTGAGTCTCTTGGGGTGACCTATGAGCAGTGGAAGATCCTCAA
TGAGATTGATGCTGGCGTGTGTGAGGAGATGACATATTCAGAGATTGAGCAACGGTACCCAGAGGAATTT
GCACTCCGAGATCAAGAGAAGTATCTGTACCGATACCCTGGTGGGGAGTCCTACCAGGACCTGGTGCAGC
GGCTGGAGCCTGTCATCATGGAGCTAGAGCGTCAAGGCAACATCCTCGTTATCTCTCACCAGGCTGTCAT
GCGCTGCCTCCTGGCCTACTTCTTGGATAAAGGCGCAGATGAGTTACCATACTTGAGGTGCCCCCTGCAC
ATCATCTTCAAACTTACTCCTGTGGCCTATGGTTGCAAAGTGGAAACAATTACTCTGAATGTGGATGCTG
TAGACACACATCGTGACAAGCCAACTGAAGTGGAGAATTTGCTGGCTGAGCATAGACACCCCTCAATGGC
ATCCCTCACTTTGCTCTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216027 representing NM_001162415
Red=Cloning site Green=Tags(s)

MSENSTFSPEDCNSSYKPHASNLRRAGKTCSWASYMTNSPTLIVMIGLPARGKTYVSKKLTRYLNWIGVP
TKVFNLGVYRREAVKSYQSYDFFRHDNEEAMKIRKQCALVALEDVKAYFTEESGQIAVFDATNTTRERRD
MILNFAKQNAFKVFFVESVCDDPDVIAANILEVKVSSPDYPERNRENVMEDFLKRIECYKVTYQPLDPDN
YDKDLSFIKVINVGQRFLVNRVQDYIQSKIVYYLMNIHVHPRTIYLCRHGESEFNLLGKIGGDSGLSVRG
KQFAHALKKFLEEQEIQDLKVWTSQLKRTIQTAESLGVTYEQWKILNEIDAGVCEEMTYSEIEQRYPEEF
ALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNILVISHQAVMRCLLAYFLDKGADELPYLRCPLH
IIFKLTPVAYGCKVETITLNVDAVDTHRDKPTEVENLLAEHRHPSMASLTLLS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001162415
ORF Size 1419 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001162415.1, NP_001155887.1
RefSeq Size 3689 bp
RefSeq ORF 1422 bp
Locus ID 18640
Cytogenetics 1 E4
Summary Synthesis and degradation of fructose 2,6-bisphosphate.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001162415" in other vectors (3)
SKU Description Size Price
MR216027 Pfkfb2 (Myc-DDK-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 2 10 ug
$289.00 $503.00
MR216027L3 Lenti ORF clone of Pfkfb2 (Myc-DDK-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 2 10 ug
$803.00
MR216027L4 Lenti ORF clone of Pfkfb2 (mGFP-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 2 10 ug
$803.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.