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Megf6 (NM_001162977) Mouse Tagged ORF Clone

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SKU
MG216019
Megf6 (tGFP-tagged) - Mouse multiple EGF-like-domains 6 (Megf6), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,545.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

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PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
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Other products for "Megf6 or NM_001162977"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Megf6
Synonyms 2600001P17Rik; Egfl3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216019 representing NM_001162977
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGTCGGGGTGGAGGCGAGGGCGTCTTGGCGCGTGGTGGCCCTGACGCTGCTGCTGCTCCCCGCCG
TGCCTGCAGCCTCCCAGCCGCTACCCCCGCGCCCGCTGCAGCCGAGCATGCCCCACGTGTGTGCAGAACA
GAAGCTGACCCTGGTGGGCCACCGCCAGCCTTGTGTGCAAGCCTTCAGCCGCGTAGTGCCTGTGTGGAGG
TCGGGCTGCGGGCAGCAGGCATGGTGTGTTGGCCAAGAGCGCAGGACTGTGTACTATATGAGCTACAGGC
AGGTGTATGCCACAGAGGCTCGCACTGTGTTCAGGTGCTGTCCTGGCTGGAGCCAGAAGCCTGGCCAGGA
AGGCTGCCTCTCAGATGTGGATGAATGTGCCAATGCCAACGGGGGCTGTGAGGGCCCGTGCTGCAACACC
GTGGGTGGCTTTTACTGTCGCTGCCCACCCGGCTACCAGCTGCAGGGTGATGGCAAAACGTGCCAAGATG
TGGATGAATGTCGATCACACAATGGTGGTTGTCAACACAGGTGTGTGAATACCCCCGGCTCTTACCTCTG
TGAGTGCAAACCCGGCTTCCGGCTCCACACAGATGGCAGGACCTGCCTGGCCATCAGCTCCTGCACTCTG
GGAAATGGTGGCTGCCAGCATCAGTGTGTACAGCTCACCGTGACACAGCACCGCTGCCAGTGCCGGCCCC
AGTACCAGCTGCAGGAGGATGGCAGGCGCTGTGTCCGGAGAAGCCCCTGTGCAGACGGCAATGGTGGCTG
TATGCATACATGCCAGGAACTCCGGGGCCTTGCCCACTGTGGCTGCCACCCAGGTTACCAGCTTGCTGCA
GACCGCAAAGCCTGTGAAGATGTGGACGAATGTGCCTTGGGTCTGGCCCAGTGTGCTCATGGCTGCCTCA
ACACTCAGGGGTCCTTTAAGTGTGTGTGTCACGCAGGCTATGAGCTGGGTGCTGATGGCCGGCAGTGTTA
CCGGATCGAGATGGAGATTGTGAACAGCTGCGAGGCTGGCAACGGAGGCTGCTCACACGGCTGTAGTCAC
ACTAGCACAGGGCCCCTGTGCACCTGCCCTCGTGGTTATGAGCTGGATGAGGACCAGAAGACCTGCATTG
ACATCGACGACTGCGCCAACTCTCCGTGCTGCCAGCAGGTGTGTGCCAACACCCCCGGTGGGTATGAGTG
TAGCTGCTTCGCCGGCTACAGACTCAACACAGACGGCTGTGGCTGTGAGGATGTTGACGAGTGTGCCTCT
GGTCACAGCGGCTGCGAACACCACTGTTCTAATCTGGCTGGCTCCTTCCAGTGCTTCTGTGAGGCTGGCT
ACCGGCTGGATGAGGACCGCAGGGGCTGCACGCCCCTAGAAGAATCCGTGGTGGACCTGGATGGCCAGCT
GCCCTTTGTCCGGCCCCTGCCCCACATTGCGGTGCTGGGGGATGAGCTTCCACAACTCTTCCAAGATGAC
TATGGGGCTGAGGAGGAGGCTGTGGCGGAGCTGCGGGGAGAACACACTCTCACTGAGAAGTTTGTCTGCT
TGGATCACTCCTTCGGCCATGACTGCAGCCTAACCTGCGATGACTGCAGGAATGGGGGGACCTGCTTCCC
GGGCCTGGATGGCTGTGACTGCCCAGAGGGCTGGACCGGGATCATCTGCAATGAGACTTGTCCTCCAGAC
ACTTTTGGGAAGAACTGTAGCTCTCCCTGCATCTGTCAGAATGGTGGGACCTGTGACCCGGTGTCAGGAG
CCTGCCGCTGTCCCCCAGGCGTCAGTGGAGCCCACTGTGAGGATGGCTGCCCCAAGGGCTTCTACGGCAA
ACACTGTCGTAAGAAATGCCACTGTGCTAACCGGGGCCGGTGCCACCGCCTCTATGGGGCCTGCCTCTGT
GACCCAGGCCTCTATGGCCGTTTCTGTCACCTTGCTTGCCCTCCCTGGGCCTTTGGACCGGGCTGTTCTG
AGGACTGCCTTTGTGAGCAGTCACACACAAGATCTTGTAACTCGAAGGATGGCAGCTGCTCCTGCAAGGC
CGGCTTCCAGGGCGAGCGCTGCGAGGCGGAGTGCGAGCCAGGCTCCTTTGGGCCAGGCTGCAGAAACCGT
TGCACCTGCCGGCCAGGTGTGGCCTGTGACCCTGTGAGTGGCGAGTGTCGGACGCAGTGTCCTCCTGGCT
ACCAGGGGGAGGACTGTGGCCAAGAGTGCCCAGTGGGGACATTTGGTGTGAACTGCTCAGGTTCCTGCTC
CTGCGTGGGGGCCCCCTGTCACCGAGTCACGGGGGAGTGTCTGTGCCCACCAGGGAAGACTGGGGAGGAC
TGTGGAGCCGACTGTCCCGAGGGCCGTTGGGGGCTCGGCTGTCAAGAGATCTGCCCTGCATGTGAGCATG
GTGCCAGCTGCGACCCTGAGACTGGAACCTGCCTGTGTCTTCCTGGTTTTGTTGGTAGCCGCTGCCAGGA
TGCTTGCCCAGCAGGCTGGTTTGGGACCGGCTGCCAGATGAGGTGTGCTTGTGCCAATGATGGGCATTGC
CATCCGGCCACTGGACGCTGTAGCTGTGCCCCTGGGTGGACTGGCCTCAGCTGCCAGAGAGCCTGTGACA
GTGGACACTGGGGGCCTGACTGCATCCACCCCTGCAACTGCAGTGCAGGCCATGGGAACTGTGACGCCGT
CAGTGGCCTCTGCCTGTGTGAGGCTGGCTACGAAGGTCCGCAGTGTGAGCAGTGGTGTCGCCAAGGCTAC
TTTGGGCCCGGCTGCGAGCAGAAGTGCAGGTGTGAGCATGGGGCCACCTGTGACCATGTCAGTGGGGCCT
GTACCTGCCCAGCTGGCTGGAGGGGAAGCTTCTGCGAGCACGCCTGTCCTGCTGGCTTCTTTGGGTTAGA
CTGTGGCAGTGCCTGCAACTGCTCCGCCGGGGCCCCCTGTGATGCTGTCACTGGCTCGTGCATCTGCCCA
GCTGGCCGCTGGGGCCCACACTGTGCCCAGACTTGCCCACCTCTCACCTTTGGGCTTAACTGCAGCCAGA
TTTGTACCTGTTTCAATGGGGCCTCTTGCGACCCTGTCCTTGGGCAGTGCCACTGTGCTCCTGGCTGGAT
GGGACCCACCTGCCTGCAGGCCTGCCCTGCTGGCCTGTATGGCAAGAACTGTCAGCATTCCTGTCTCTGC
CGAAATGGAGGGAACTGTGACCCCATCTTGGGCCAGTGCACGTGCCCAGAGGGTTGGACCGGCTTGGCCT
GTGAGAACGAGTGCCTTCCTGGACACCATGGGGCTGGTTGCCGGCTCAACTGCAGTTGCCTTAATGGGGG
CACATGTGACCGTCTTACTGGCCACTGCCGCTGCCCAACTGGCTGGACTGGGGACAAGTGTCAGAGCCCC
TGCGTCAGTGGCATGTTTGGGGTTCACTGTGAGGAGCACTGTGCCTGCCGGAAAGGTGCCACCTGTCACC
ATGTCACGGGGGCCTGCCTCTGTCCCCCAGGATGGAGGGGCTCACACTGTGAACAGGCCTGTCCACGTGG
CTGGTTTGGAGAGGCCTGTGCTCAGCGTTGCCACTGCCCGCCTGGTGCCTCCTGCCACCATGTCTCTGGA
GAGTGCCACTGTCCACCCGGCTTCACTGGGCCTGGCTGTGAGCAGGCCTGCCAGCCTGGCACCTTTGGCA
AGGACTGTGAGCACCCGTGCCAATGTCCTGGTGAGACCTGGGCCTGCCACCCTGCTTCAGGGGCCTGCGT
ATGTGCTGCTGGTTACCATGGCACCGACTGTCAGCAACGATGCCCATCTGGGCGCTATGGGCCAGGCTGT
GAACAAATTTGTAAGTGTCTCAATGGTGGGACCTGCGATCCAGCCACGGGAGCCTGCTACTGCCCTGCTG
GGTTCCTTGGGGCCGACTGTAGCCTTGCCTGTCCACAGGGTCGCTTCGGCCCCAGCTGTGCCCACGTGTG
TACATGCGGGCAAGGGGCGGCATGTGACCCAGTGTCGGGGACTTGCATCTGTCCTCCCGGGAAGACGGGA
GGCCATTGTGAGCGCGGCTGTCCCCAGGACCGGTTTGGCAAGGGCTGTGAACACAAGTGTGCCTGCAGGA
ATGGGGGCCTGTGTCATGCTACCAATGGCAGCTGCTCCTGCCCCCTGGGCTGGATGGGGCCACACTGTGA
GCACGCCTGCCCTGCTGGGCGCTATGGTGCTGCCTGCCTCCTGGAGTGTTCCTGTCAGAACAATGGCAGC
TGTGAGCCCACCTCCGGCGCTTGCCTCTGTGGCCCTGGCTTCTATGGTCAAGCTTGTGAAGACACCTGCC
CTGCCGGCTTCCATGGATCTGGTTGCCAGAGAGTTTGCGAGTGTCAACAGGGCGCTCCCTGTGACCCTGT
CAGTGGCCGGTGCCTCTGCCCTGCTGGCTTCCATGGCCAGTTCTGCGAGAGGGGGTGCAAGCCAGGCTTT
TTTGGAGATGGCTGCCTGCAGCAGTGTAACTGCCCCACGGGTGTGCCCTGTGATCCCATCAGCGGCCTCT
GCCTTTGCCCACCAGGGCGTGCAGGAACCACATGTGACCTAGATTGCAGAAGAGGCCGCTTTGGGCCGGG
CTGTGCCCTGCGCTGTGATTGTGGGGGTGGGGCTGACTGCGACCCCATCAGTGGGCAGTGCCACTGTGTG
GACAGCTACACGGGACCCACTTGCCGGGAAGTGCCCACACAGCTGTCCTCTATCAGACCAGCACCCCAGC
ACTCCAGCAGCAAGGCCATGAAGCAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216019 representing NM_001162977
Red=Cloning site Green=Tags(s)

MPVGVEARASWRVVALTLLLLPAVPAASQPLPPRPLQPSMPHVCAEQKLTLVGHRQPCVQAFSRVVPVWR
SGCGQQAWCVGQERRTVYYMSYRQVYATEARTVFRCCPGWSQKPGQEGCLSDVDECANANGGCEGPCCNT
VGGFYCRCPPGYQLQGDGKTCQDVDECRSHNGGCQHRCVNTPGSYLCECKPGFRLHTDGRTCLAISSCTL
GNGGCQHQCVQLTVTQHRCQCRPQYQLQEDGRRCVRRSPCADGNGGCMHTCQELRGLAHCGCHPGYQLAA
DRKACEDVDECALGLAQCAHGCLNTQGSFKCVCHAGYELGADGRQCYRIEMEIVNSCEAGNGGCSHGCSH
TSTGPLCTCPRGYELDEDQKTCIDIDDCANSPCCQQVCANTPGGYECSCFAGYRLNTDGCGCEDVDECAS
GHSGCEHHCSNLAGSFQCFCEAGYRLDEDRRGCTPLEESVVDLDGQLPFVRPLPHIAVLGDELPQLFQDD
YGAEEEAVAELRGEHTLTEKFVCLDHSFGHDCSLTCDDCRNGGTCFPGLDGCDCPEGWTGIICNETCPPD
TFGKNCSSPCICQNGGTCDPVSGACRCPPGVSGAHCEDGCPKGFYGKHCRKKCHCANRGRCHRLYGACLC
DPGLYGRFCHLACPPWAFGPGCSEDCLCEQSHTRSCNSKDGSCSCKAGFQGERCEAECEPGSFGPGCRNR
CTCRPGVACDPVSGECRTQCPPGYQGEDCGQECPVGTFGVNCSGSCSCVGAPCHRVTGECLCPPGKTGED
CGADCPEGRWGLGCQEICPACEHGASCDPETGTCLCLPGFVGSRCQDACPAGWFGTGCQMRCACANDGHC
HPATGRCSCAPGWTGLSCQRACDSGHWGPDCIHPCNCSAGHGNCDAVSGLCLCEAGYEGPQCEQWCRQGY
FGPGCEQKCRCEHGATCDHVSGACTCPAGWRGSFCEHACPAGFFGLDCGSACNCSAGAPCDAVTGSCICP
AGRWGPHCAQTCPPLTFGLNCSQICTCFNGASCDPVLGQCHCAPGWMGPTCLQACPAGLYGKNCQHSCLC
RNGGNCDPILGQCTCPEGWTGLACENECLPGHHGAGCRLNCSCLNGGTCDRLTGHCRCPTGWTGDKCQSP
CVSGMFGVHCEEHCACRKGATCHHVTGACLCPPGWRGSHCEQACPRGWFGEACAQRCHCPPGASCHHVSG
ECHCPPGFTGPGCEQACQPGTFGKDCEHPCQCPGETWACHPASGACVCAAGYHGTDCQQRCPSGRYGPGC
EQICKCLNGGTCDPATGACYCPAGFLGADCSLACPQGRFGPSCAHVCTCGQGAACDPVSGTCICPPGKTG
GHCERGCPQDRFGKGCEHKCACRNGGLCHATNGSCSCPLGWMGPHCEHACPAGRYGAACLLECSCQNNGS
CEPTSGACLCGPGFYGQACEDTCPAGFHGSGCQRVCECQQGAPCDPVSGRCLCPAGFHGQFCERGCKPGF
FGDGCLQQCNCPTGVPCDPISGLCLCPPGRAGTTCDLDCRRGRFGPGCALRCDCGGGADCDPISGQCHCV
DSYTGPTCREVPTQLSSIRPAPQHSSSKAMKH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001162977
ORF Size 4716 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001162977.1, NP_001156449.1
RefSeq Size 6876 bp
RefSeq ORF 4719 bp
Locus ID 230971
Cytogenetics 4 E2
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Documents
Resources
"NM_001162977" in other vectors (4)
SKU Description Size Price
MC224701 Megf6 (untagged) - Mouse multiple EGF-like-domains 6 (Megf6), (10ug) 10 ug
$1,480.00
MR216019 Megf6 (Myc-DDK-tagged) - Mouse multiple EGF-like-domains 6 (Megf6) 10 ug
$589.00 $1,345.00
MR216019L3 Lenti ORF clone of Megf6 (Myc-DDK-tagged) - Mouse multiple EGF-like-domains 6 (Megf6) 10 ug
$1,645.00
MR216019L4 Lenti ORF clone of Megf6 (mGFP-tagged) - Mouse multiple EGF-like-domains 6 (Megf6) 10 ug
$1,645.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.