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Folh1 (NM_016770) Mouse Tagged ORF Clone

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SKU
MG216018
Folh1 (tGFP-tagged) - Mouse folate hydrolase (Folh1) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $1,233.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Folh1
Synonyms GCP2; mopsm
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216018 representing NM_016770
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGAACGCACTGCAGGACAGAGACTCCGCGGAGGTCCTGGGACACCGCCAGCGCTGGCTCCGTGTTG
GGACACTGGTGCTGGCTTTAACCGGAACCTTCCTCATTGGCTTCCTCTTTGGGTGGTTTATAAAACCTTC
CAATGAAGCTACTGGTAATGTTTCCCATTCTGGCATGAAGAAGGAGTTTTTGCATGAATTGAAGGCTGAG
AACATCAAAAAATTTTTATACAATTTCACACGGACACCACACTTGGCAGGAACACAAAATAATTTTGAGC
TTGCAAAGCAAATTCATGACCAGTGGAAAGAATTTGGCCTGGATTTGGTTGAGTTATCCCATTACGATGT
CTTGCTGTCCTATCCAAATAAAACTCATCCTAACTATATCTCAATAATTAATGAAGATGGAAATGAGATT
TTCAAAACATCATTATCTGAACAGCCACCCCCAGGATATGAGAATATATCAGATGTAGTGCCACCATACA
GTGCCTTCTCTCCACAAGGGACACCAGAGGGTGATCTAGTGTATGTCAACTATGCACGAACTGAAGACTT
CTTTAAACTGGAACGGGAAATGAAGATCAGTTGTTCTGGGAAGATTGTGATTGCCAGATATGGGAAAGTG
TTCAGAGGAAATATGGTTAAAAATGCTCAACTGGCAGGGGCAAAAGGAATGATTCTGTACTCAGACCCTG
CTGACTACTTTGTTCCTGCGGTGAAGTCCTATCCAGATGGCTGGAACCTCCCTGGAGGTGGTGTCCAACG
TGGAAATGTCTTAAATCTTAATGGTGCAGGTGACCCGCTCACACCAGGTTACCCAGCAAATGAACATGCT
TATAGGCATGAGTTGACAAACGCTGTTGGCCTTCCAAGTATTCCTGTCCATCCTATTGGATATGATGATG
CACAGAAACTCTTAGAACACATGGGTGGTCCAGCACCCCCTGACAGTAGCTGGAAGGGAGGATTAAAAGT
GCCTTACAACGTGGGACCTGGCTTTGCTGGAAACTTTTCAACACAAAAGGTCAAGATGCATATTCACTCT
TACACTAAAGTGACAAGAATCTATAATGTCATTGGCACCCTCAAAGGAGCTCTGGAACCAGACAGATATG
TTATTCTTGGAGGTCACCGAGATGCTTGGGTATTTGGTGGCATTGACCCTCAGAGTGGAGCAGCTGTTGT
TCATGAAATTGTGCGGAGCTTTGGAACCCTGAAGAAGAAAGGACGGAGGCCTAGAAGGACAATTTTGTTT
GCAAGCTGGGATGCAGAAGAATTTGGCCTTCTTGGTTCTACTGAGTGGGCAGAGGAACATTCAAGACTCC
TACAAGAGCGAGGTGTGGCTTATATTAATGCTGATTCTTCCATAGAAGGAAATTACACTCTAAGAGTTGA
TTGCACACCACTGATGTACAGCTTAGTGTACAACCTAACAAAAGAGCTGCAAAGCCCAGATGAAGGTTTT
GAAGGAAAATCTCTTTATGACAGCTGGAAAGAAAAGAGTCCTTCACCTGAGTTCATTGGAATGCCCAGAA
TTAGCAAGCTGGGGTCTGGCAATGATTTTGAAGTGTTCTTCCAAAGACTTGGAATTGCTTCAGGCAGAGC
CCGATATACTAAAAATTGGAAAACTAACAAAGTCAGCAGCTATCCTCTCTATCACAGTGTCTATGAAACA
TATGAGCTGGTAGTAAAATTTTATGACCCAACATTTAAATACCACCTCACTGTGGCCCAGGTTCGAGGAG
CAATGGTATTTGAACTTGCCAATTCTATAGTGCTTCCCTTTGACTGCCAAAGTTATGCTGTAGCTCTGAA
GAAGTATGCTGACACTATCTACAATATTTCAATGAAACATCCACAAGAAATGAAGGCTTACATGATATCA
TTTGATTCACTGTTTTCTGCAGTCAATAATTTTACAGATGTTGCATCTAAGTTCAATCAGAGACTGCAAG
AGTTAGACAAAAGCAACCCCATATTACTGAGAATTATGAATGACCAGCTGATGTATCTGGAACGTGCATT
CATTGATCCTTTAGGCTTACCAGGAAGGCCTTTCTACAGGCATATCATCTATGCTCCAAGCAGCCACAAC
AAGTATGCAGGAGAATCATTCCCTGGGATTTATGATGCCCTTTTTGATATAAGTAGCAAAGTCAATGCTT
CTAAGGCCTGGAACGAAGTGAAGAGACAGATTTCTATTGCAACCTTTACAGTGCAAGCTGCAGCAGAGAC
TCTGAGGGAAGTAGCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216018 representing NM_016770
Red=Cloning site Green=Tags(s)

MWNALQDRDSAEVLGHRQRWLRVGTLVLALTGTFLIGFLFGWFIKPSNEATGNVSHSGMKKEFLHELKAE
NIKKFLYNFTRTPHLAGTQNNFELAKQIHDQWKEFGLDLVELSHYDVLLSYPNKTHPNYISIINEDGNEI
FKTSLSEQPPPGYENISDVVPPYSAFSPQGTPEGDLVYVNYARTEDFFKLEREMKISCSGKIVIARYGKV
FRGNMVKNAQLAGAKGMILYSDPADYFVPAVKSYPDGWNLPGGGVQRGNVLNLNGAGDPLTPGYPANEHA
YRHELTNAVGLPSIPVHPIGYDDAQKLLEHMGGPAPPDSSWKGGLKVPYNVGPGFAGNFSTQKVKMHIHS
YTKVTRIYNVIGTLKGALEPDRYVILGGHRDAWVFGGIDPQSGAAVVHEIVRSFGTLKKKGRRPRRTILF
ASWDAEEFGLLGSTEWAEEHSRLLQERGVAYINADSSIEGNYTLRVDCTPLMYSLVYNLTKELQSPDEGF
EGKSLYDSWKEKSPSPEFIGMPRISKLGSGNDFEVFFQRLGIASGRARYTKNWKTNKVSSYPLYHSVYET
YELVVKFYDPTFKYHLTVAQVRGAMVFELANSIVLPFDCQSYAVALKKYADTIYNISMKHPQEMKAYMIS
FDSLFSAVNNFTDVASKFNQRLQELDKSNPILLRIMNDQLMYLERAFIDPLGLPGRPFYRHIIYAPSSHN
KYAGESFPGIYDALFDISSKVNASKAWNEVKRQISIATFTVQAAAETLREVA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016770
ORF Size 2256 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016770.3, NP_058050.3
RefSeq Size 3047 bp
RefSeq ORF 2259 bp
Locus ID 53320
UniProt ID O35409
Cytogenetics 7 D3
Summary Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides (By similarity). In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_016770" in other vectors (4)
SKU Description Size Price
MC221298 Folh1 (untagged) - Mouse folate hydrolase (Folh1), transcript variant 1, (10ug) 10 ug
$1,034.00
MR216018 Folh1 (Myc-DDK-tagged) - Mouse folate hydrolase (Folh1), transcript variant 1 10 ug
$1,033.00
MR216018L3 Lenti ORF clone of Folh1 (Myc-DDK-tagged) - Mouse folate hydrolase (Folh1), transcript variant 1 10 ug
$1,333.00
MR216018L4 Lenti ORF clone of Folh1 (mGFP-tagged) - Mouse folate hydrolase (Folh1), transcript variant 1 10 ug
$1,333.00

file-alt Citations

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