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Thnsl1 (NM_177588) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216015
Thnsl1 (tGFP-tagged) - Mouse threonine synthase-like 1 (bacterial) (Thnsl1) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $884.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Thnsl1
Synonyms AW413632
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216015 representing NM_177588
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGACCTTGACCAGATGTCACCATTTGAAACAGATAGCACAGGAGTGTCTTTCTAGCCTGCTGGTTA
AAGTGCAGAGCCGCACACAGCTCCTGCTTCCACGAGCCTCTGCACGTGCAGAATCAGGGAAGTCATGGCA
CTCCACCCACTCTCTTGTTGGAGACAAAAATATTGTCCTGATGGGGCCGCCTGGTTCTGGGAAAACAACA
GTTGGCAGAATATTAGGTGACAAACTAGGCTGTTGTGTCATCGATGTAGATAGTGATGTCCTTGAGAAAG
CCTGGAATATGAGCGCATCTGAAAAATTGCAGGATGTTGGTAATGAGCGATTTTTAGAAGAGGAAGGAAA
AACAGTGTTAAACTTGTCTGCATCTGGAAGTGTGATTTCCCTCAGTGGGTCCAATCCCATGCATGATGCT
AGCATGTGGCATCTGAAGAAAAATGGAATTGTTGTATACCTAGATGTGCCTCTAACAGATATAATTAGTC
GTCTGAAATCAATGAGAATTGATAGGATTGTTGGCCAGAACACTGGAGCATCTCTGAGAGACTCGCTGAA
GCACGTAAGACTGTACTATAAGAAGTGGTACGATGCCCGAGTCTTCTGTGAGAGTGGGGCTTCAGCTGAG
GAGGTTGCAGACAAAGTGCTTGATGTGGTTAAAAGATACCAAGACGTGGATTCAGAGACATTCATTTCAA
CAAGGCATGTTTGTCTTAAAGATCATGACAAGAAGTTTCCACCAAAATACTTCAGTGAAGCTGTAGTTGA
GGGATTGGCTTCTGATGGTGGCCTCTTTGTTCCTGAGAAGGAGTTTCCAAAACTAAGTCCTGGGGAGTGG
AATAACCTAATAGGAGCAACCTACATAGAAAGAGCACAGGTGCTCTTGGAAAGGTGCATTCACCCTGCGG
ACATACCTGCTGCCAAATTAGGAGAAATGATTGAAACTGCTTATGGGGAAAACTTTGCCTGCTCCAAAGT
TGCCCCTGTCAGGCACCTTTCTGGCAATCAGTTCATCTTGGAGTTGTTCTATGGCCCAACGGGGTCCTTT
AAGGATTTGTCTTTACAGCTTATGCCTCATATTTTTGCCTACTGTATCCCACCAGGTTGCAATTATGTGA
TACTTGTAGCCACTTCAGGAGACACTGGGAGTGCAGTCTTAAATGGCTTTAGCCATCTTAATAAAAATGA
TAAGGAAAGAATAGCTGTGGTCACATTTTTTCCAGAGAACGGAGTTAGTGATTTCCAGAAAGCAGAGATA
ATTGGCAGTCAGAGAGAAAATGGTTGGGCAATAGGTGTCAGGTCAGATTTTGATTTTTGCCAGACAGCTA
TTAGAAAAATATTTAATGATTCTGATTTTACTGGCTTTCTGGCTGTGGAATATGGAACAATCTTAAGTTC
AGCTAATTCTATAAATTGGGCTCGACTACTTCCGCAGATAGTTTATCATGCCTCTGCATACCTTGAGCTT
GTTAACCAACGGTTTATTTCTTTTGGAAGCCCAGTAGATGTCTGTGTTCCCACAGGAAACTTTGGGAACG
TGTTGGCGGCAGTGTATGCCAAGATGATGGGAATCCCTATTCGAAAATTTATCTGTGCCTCTAATCAGAA
CCACGTTTTGACTGACTTCATAAAAACTGGACATTATGATCTAAGGAATAGGAAATTAGCACAAACCTTT
TCACCATCAATAGATATTCTCAAGTCTTCCAACCTGGAGCGACATTTATACTTGATGGCTAACAAAGACG
GACAGTTAATGGCAAATTTATACCATCAGTTAGAGAGTCAGCTTCACTTCCGGATTGAGAAGATGCTGGT
CGAGAAGCTGCAGCAAGAGTTTGTTGCCGACTGGTGCTCTGAGGGAGAATGCCTCGCAGCTATCAGCACT
ACCTACAATGCATCAGGCTATATTTTGGATCCACATACCGCTGTTGCAAAAGTGGTTGCAGACAAGATGC
AAGACAAAAGCTGCCCAGTGCTCATTGCATCCACAGCTCACTACTCCAAGTTTGCCCCTGCTATCATGCA
GGCACTGGGGATTAAAGAACTCAACCAGACGTCGTCCAGCCAGCTTTACTTACTGAGCTCGTACAATGCA
TTGCCCCCTCCTCACGAGGCTCTGCTAGAGAGAATGAAGCAGAAGGAGAAGATGGATTACCAGGTTTGTG
TAGCTGATGTGGATGTCCTGAAGAGTCATGCAGAAAAACTCATCCAAAATTGGTTTGTAAGGAAGTCTGA
G

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216015 representing NM_177588
Red=Cloning site Green=Tags(s)

MLTLTRCHHLKQIAQECLSSLLVKVQSRTQLLLPRASARAESGKSWHSTHSLVGDKNIVLMGPPGSGKTT
VGRILGDKLGCCVIDVDSDVLEKAWNMSASEKLQDVGNERFLEEEGKTVLNLSASGSVISLSGSNPMHDA
SMWHLKKNGIVVYLDVPLTDIISRLKSMRIDRIVGQNTGASLRDSLKHVRLYYKKWYDARVFCESGASAE
EVADKVLDVVKRYQDVDSETFISTRHVCLKDHDKKFPPKYFSEAVVEGLASDGGLFVPEKEFPKLSPGEW
NNLIGATYIERAQVLLERCIHPADIPAAKLGEMIETAYGENFACSKVAPVRHLSGNQFILELFYGPTGSF
KDLSLQLMPHIFAYCIPPGCNYVILVATSGDTGSAVLNGFSHLNKNDKERIAVVTFFPENGVSDFQKAEI
IGSQRENGWAIGVRSDFDFCQTAIRKIFNDSDFTGFLAVEYGTILSSANSINWARLLPQIVYHASAYLEL
VNQRFISFGSPVDVCVPTGNFGNVLAAVYAKMMGIPIRKFICASNQNHVLTDFIKTGHYDLRNRKLAQTF
SPSIDILKSSNLERHLYLMANKDGQLMANLYHQLESQLHFRIEKMLVEKLQQEFVADWCSEGECLAAIST
TYNASGYILDPHTAVAKVVADKMQDKSCPVLIASTAHYSKFAPAIMQALGIKELNQTSSSQLYLLSSYNA
LPPPHEALLERMKQKEKMDYQVCVADVDVLKSHAEKLIQNWFVRKSE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177588
ORF Size 2241 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177588.2, NP_808256.1
RefSeq Size 3899 bp
RefSeq ORF 2244 bp
Locus ID 208967
UniProt ID Q8BH55
Cytogenetics 2 A3
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Documents
Resources
"NM_177588" in other vectors (4)
SKU Description Size Price
MC221324 Thnsl1 (untagged) - Mouse threonine synthase-like 1 (bacterial) (Thnsl1), transcript variant 1, (10ug) 10 ug
$753.00
MR216015 Thnsl1 (Myc-DDK-tagged) - Mouse threonine synthase-like 1 (bacterial) (Thnsl1), transcript variant 1 10 ug
$589.00 $684.00
MR216015L3 Lenti ORF clone of Thnsl1 (Myc-DDK-tagged) - Mouse threonine synthase-like 1 (bacterial) (Thnsl1), transcript variant 1 10 ug
$984.00
MR216015L4 Lenti ORF clone of Thnsl1 (mGFP-tagged) - Mouse threonine synthase-like 1 (bacterial) (Thnsl1), transcript variant 1 10 ug
$984.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.