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Tnrc6b (NM_177124) Mouse Tagged ORF Clone

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SKU
MG215989
Tnrc6b (tGFP-tagged) - Mouse trinucleotide repeat containing 6b (Tnrc6b) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,840.00
8 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

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PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
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Other products for "Tnrc6b or NM_177124"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tnrc6b
Synonyms 2700090M07Rik; A730065C02Rik; AI848765; D230019K20Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215989 representing NM_177124
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAGAGAAGGAGCAAGAAAGGGAAGAACAGTTAATGGAAGACAAGAAAAGGAAGAAAGAGGATAAAA
AGAAAAAAGAAGCCACCCAGAAGGTCACGGAACAAAAAACCAAAGTGCCCGAAGTGACGAAACCAAGTTT
AAGCCAACCAACGGCCGCCAGCCCAATTGGCAGCTCTCCATCGCCACCAGTCAATGGTGGCAACAATGCC
AAAAGGGTGGCAGTGCCGAACGGACAACCGCCAAGCGCCGCCCGCTACATGCCTCGGGAGGTGCCACCGC
GATTCCGTTGCCAGCAGGACCATAAAGTGTTACTAAAACGTGGGCAGCCCCCTCCACCGTCCTGCATGCT
CCTCGGGGGTGGGGCGGGGCCTCCTCCCTGCACAGCACCTGGAGCAAACCCCAACAACAACGCACAAGTG
ACAGGAGCGCTGCTGCAGAGTGAGAGTGGGACTGCACCAGAGTCAACCCTTGGAGGTGCTGCTGCTTCAA
ATTATGCAAATTCCACTTGGGGCCCGGGAGCCTCCTCCAACAGCGGCGCCTCCCCCAACCCAATTCACAT
CTGGGACAAGGTGATTGTAGACGGGTCTGACATGGAAGAGTGGCCTTGTATTGCCAGCAAAGACACTGAA
TCTTCTTCCGAAAACACCACCGATAACAACAGTGCCTCGAACCCTGGCTCTGAGAAGAGCAGTCTGCCAG
GAAGCACCACTAGTAACAAAGGCAAAGGGAGCCAATGCCAGGCTGCAAGTTCTGGGAACGAATGTAATCT
TGGGGTCTGGAAATCTGACCCCAAGGCTAAGTCTGTTCAACCTCCCAACTCTACTTCAGATAGCAACAAT
GGACTAGGAAACTGGAGGAGCACGAGTGGTCAGGATAGAATTGGACCTGGCTCTGGCTTCAGCAACTTTA
ACCCAAATAGCAACCCATCTGCCTGGCCAGCATTGGTCCAAGAAGGGACTTCTAGAAAAGGGGCATTGGA
AACAGAAAGTAGTAGTTCCAGTGCACAAGTTAGCACAGTAGGTCAGGCATCCAGGGAACAGCAGTCAAAG
ATGGAAAATGCGGGTGTTAATTTTGTTGTCTCTGGCAGAGAACAGGCTCAAATTCATAACACTGATGGAC
CAAAAAATGGAAACACTAACTCCTTGAACTTAAGTTCACCAAACCCCATGGAAAATAAGGGAATGCCCTT
TGGAATGGGCTTGGGGAACACCTCCAGGAGCACTGATGCCCCTTCACAAAGCACTGGAGATCGAAAGACT
GGGAGTGTTGGGTCTTGGGGTGCAGCTAGGGGGCCTTCTGGAACTGACACAGTCTCTGGACAAAGCAATT
CTGGAAATAATGGGAACAATGGAAAAGACAGAGAGGACTCCTGGAAAGGAGCTTCTGTTCCAAAACCAAC
TGGGTCAAAAAGTGACTCTTGGGACAACAATAACAGGTCTACGGGTGGGTCCTGGAACTTTGGCCCTCAG
GACAATAATGACAACAAATGGGGTGAAGGGAACAAAATGACATCTGGGGTCTCTCAGGGAGAATGGAAAC
AGCCGACTGGGTCTGATGAGTTGAAAATTGGAGAATGGAGTGGTCCAAACCAACCAAATTCTAGCACTGG
AGCATGGGACAATCAAAAGGGCCACCCCCTCCCTGAAAACCAAGGCAATGCCCAGGCTCCCTGTTGGGGA
AGATCTTCCAGCTCCGCAGGAAGTGAAGTTGGAGGTCAAAGCACTGGAAGCAACCACAAAGCAGGAAGTA
GTGACAGTCATAATTCTGGTCGTCGGTCATATAGGCCCACGCATCCTGATTGCCAGGCTGTCTTGCAGAC
TCTTTTGAGCCGGACTGATTTGGACCCTAGGGTGCTCTCAAACACTGGCTGGGGCCAAACTCAAATTAAG
CAGGACACAGTGTGGGATATTGAAGAGGTGCCAAGGCCTGAGGGGAAATCTGACAAAGGAACTGAGGGGT
GGGAGAGCGCTGCCACACAGACCAAGAACTCAGGGGGCTGGGGAGATGCACCCAGCCAAAGCAATCAAAT
GAAGTCTGGATGGGGGGAGCTCTCAGCCTCGACAGAGTGGAAAGACCCCAAGAGCACAGGAGGCTGGAAT
GACTATAAGAACAACAACTCTTCTAACTGGGGAGGAGGCCGAGCAGATGAAAAGACACCTTCCTCTTGGA
ATGAGAGTTCTTGTAAGGATCAGGGGTGGGGAGGTGGGCGCCAACCCAACCAAGGATGGACTTCTGGGAA
GAATGGTTGGGGCGAGGAGGTGGATCAAGTGAAAAATAATAATTGGGAAAGTTCTGCCAATAAGCCTGTG
TCTGGGTGGGGTGAAGGAGGACAGAATGAAATTGGAACTTGGGGTAATGGTGGGAATACAAACCTAGCAT
CAAAAGGTGGGTGGGAAGATTGCAAAAGATCCCCTGCATGGAATGAGACAGGCAGACAGCCCAATTCCTG
GAATAAACAACACCAACAGCAGCAGCAGCCACCGCCACCACAGCCAGAGGCTTCTGGTTCATGGGGAGGC
CCACCCCCGCCACCTCAAGGCAATGTTCGACCTTCTAATTCCAACTGGAGCAGTGGACCCCAGCCTACAA
CCCCTAAGGATGATGAACCCAGTGGTTGGGAGGAGCCATCCCCACAGTCAATTAGTCGGAAAATGGACAT
TGATGATGGCACTTCAGCTTGGGGAGACCCTAACAGTTATAACTACAAGAATGTGAACCTTTGGGATAAG
AATTCTCAAGGGGGCCCAGCACCTCGAGAACCAAACCTGCCTACCCCAATGACCGGGAAATCAGCATCAG
TCTGGAGCAAAAGCACACCACCTGCTCCAGATAATGGGACTTCAGCCTGGGGTGAGCCAAATGAAAGCAG
TCCTGGGTGGGGTGAAATGGATGATGCAGGAGCATCAACCACAGGCTGGGGAAACACACCTGCCAACGCT
CCCAATGCCATGAAACCCAATTCCAAATCTATGCAAGACGGCTGGGGGGAGAGTGACGGACCAGTCACAG
GAGCTCGCCATCCTAGCTGGGAAGAGGAGGATGACGGAGGAGTCTGGAACACCGCCGGCTCTCAGGGAAG
CACTTCCTCCCACAACTCAGCAAGCTGGGGACAAGGAGGAAAGAAACAAATGAAGTGCTCACTAAAAGGA
GGAAACAATGATTCATGGATGAATCCTCTTGCCAAACAGTTTTCAAATATGGGATTGCTGAGTCAAACTG
AGGATAACCCAAGCAGCAAAATGGATTTGTCTGTAGATAAGAAATTTGATGTGGACAAGAGAACAATGAA
TCTCGGGGATTTTAATGATATCATGAGGAAGGATCGACCTGGGTTCCGTCCACCTAATTCCAAAGACTTG
GGAACCACAGACAGTGGGCCTTATTTTGAGAAGGGCGGCAGTCATGGTTTGTTTGGAAACAGCACAGCAC
AATCGAGAGGCCTGCACACTCCAGTGCAGCCACTAAGTTCTTCTCCTGGTCTCCGGGCGCAAGTGCCTCC
CCAGTTTATCTCCCCCCAGGTTTCTGCCTCAATGCTCAAGCAGTTCCCCAACAGTGGCCTGAATCCAGGT
CTCTTTAATGTGGGGCCCCAGCTATCTCCTCAACAGATTGCCATGCTGAGCCAGCTTCCACAGATACCCC
AGTTTCAGTTGGCATGTCAGCTTCTCCTGCAGCAGCAGCAGCAACAGCAGCAGTTGCTACAGAACCAGAG
AAAGATTTCTCAAGCAGTGCGCCAGCAGCAAGAACAGCAGCTGGCAAGAATGGTGAGTGCTCTCCAGCAG
CAGCAACAGCAGCAGCAGCAGCAGCAGAGGCAGCCTAGCATGAAGCATTCACCTTCTCATCCTGTTGGGC
CTAAGCCACATTTGGACAACATGGTACCCAATGCGCTGAATGTGGGGCTCCCAGACCTTCCAACCAAAGG
GCCAATTCCTGGATATGGTTCTGGCTTCAGCTCTGGTGGCATGGACTATGGTATGGTTGGTGGCAAGGAG
GCTGGGACCGAGTCTCGCTTCAAACAGTGGACCTCAATGATGGAAGGGCTGCCATCTGTGGCCACACAAG
AAGCCACCATGCACAAAAACGGCGCTATAGTGGCCCCTGGTAAGACCCGAGGAGGTTCACCATACAACCA
GTTTGATATAATCCCAGGTGACACACTGGGTGGCCATACGGGTCCTGCTGGTGATAGCTGGTTACCTGCC
AAATCTCCACCGACTAATAAAATCGGAAGTAAATCCAGCAATGCCAGTTGGCCTCCAGAATTCCAACCAG
GAGTGCCATGGAAAGGTATCCAAAATATTGACCCTGAATCTGACCCCTATGTCACCCCAGGAAGTGTGCT
AGGGGGTACAACCACATCTCCCATTGTAGATACTGACCACCAACTGCTGCGGGATAACACCACAGGGTCT
AATTCCTCCCTCAACACCTCGCTGCCTTCACCTGGTGCCTGGCCCTACAGTGCCTCTGACAACTCCTTTA
CCAACGTTCATAGCACTTCAGCAAAGTTCCCTGATTATAAATCCACGTGGTCCCCAGATCCCATAGGACA
CAACCCCACTCATCTCTCCAATAAGATGTGGAAAAATCACATCTCCTCCAGGAACACTACACCGCTGACC
CGCCCACCTCCTGGCCTGACCAACCCCAAACCGGCATCTCCTTGGAGCAGCACAGCACCGCGATCGGTCA
GGGGGTGGGGGACACAGGACTCCCGGATTGCCTCGGCCTCTACCTGGAGTGATGGTGGCTCTGTCCGTCC
TAGTTACTGGCTGGTTCTCCACAATCTCACCCCACAGATTGATGGGTCAACCCTGAGAACGATCTGCATG
CAGCATGGCCCACTGCTGACATTCCATCTGAACCTAACCCAGGGCACTGCCCTGATCCGATACAGCACCA
AACAAGAGGCAGCCAAGGCCCAGACTGCACTGCACATGTGTGTGTTGGGAAACACTACCATCCTGGCCGA
GTTTGCCACTGAGGATGAAGTCAGCCGCTTTCTGGCTCAAGCTCAGCCCCCTACGCCTGCAGCCACCCCG
AGTGCACCTGCCACAGGGTGGCAGTCCCTGGAGACCAGCCAGAACCAAGCAGACCCCGTTGGACCTGCGC
TGAACCTTTTCGGTGGGTCCACCGGGCTCGGGCAGTGGAGCAGCAGCGCTGGTGGCAGCAGTGGGGCCGA
TCTTGCCGGCACTTCCTTGTGGGGCCCCCCAAACTATTCTTCTAGCTTGTGGGGAGTCCCGACGGTGGAA
GATCCCCATAGGATGGGCAGCCCTGCTCCTTTACTACCTGGTGACCTTCTGGGAGGAGGGTCGGATTCAA
TC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215989 representing NM_177124
Red=Cloning site Green=Tags(s)

MREKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNA
KRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLLKRGQPPPPSCMLLGGGAGPPPCTAPGANPNNNAQV
TGALLQSESGTAPESTLGGAAASNYANSTWGPGASSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTE
SSSENTTDNNSASNPGSEKSSLPGSTTSNKGKGSQCQAASSGNECNLGVWKSDPKAKSVQPPNSTSDSNN
GLGNWRSTSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGTSRKGALETESSSSSAQVSTVGQASREQQSK
MENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGLGNTSRSTDAPSQSTGDRKT
GSVGSWGAARGPSGTDTVSGQSNSGNNGNNGKDREDSWKGASVPKPTGSKSDSWDNNNRSTGGSWNFGPQ
DNNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWG
RSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIK
QDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKSTGGWN
DYKNNNSSNWGGGRADEKTPSSWNESSCKDQGWGGGRQPNQGWTSGKNGWGEEVDQVKNNNWESSANKPV
SGWGEGGQNEIGTWGNGGNTNLASKGGWEDCKRSPAWNETGRQPNSWNKQHQQQQQPPPPQPEASGSWGG
PPPPPQGNVRPSNSNWSSGPQPTTPKDDEPSGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDK
NSQGGPAPREPNLPTPMTGKSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDAGASTTGWGNTPANA
PNAMKPNSKSMQDGWGESDGPVTGARHPSWEEEDDGGVWNTAGSQGSTSSHNSASWGQGGKKQMKCSLKG
GNNDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLSVDKKFDVDKRTMNLGDFNDIMRKDRPGFRPPNSKDL
GTTDSGPYFEKGGSHGLFGNSTAQSRGLHTPVQPLSSSPGLRAQVPPQFISPQVSASMLKQFPNSGLNPG
LFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQQQQQQQQLLQNQRKISQAVRQQQEQQLARMVSALQQ
QQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPNALNVGLPDLPTKGPIPGYGSGFSSGGMDYGMVGGKE
AGTESRFKQWTSMMEGLPSVATQEATMHKNGAIVAPGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPA
KSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNIDPESDPYVTPGSVLGGTTTSPIVDTDHQLLRDNTTGS
NSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFPDYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLT
RPPPGLTNPKPASPWSSTAPRSVRGWGTQDSRIASASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICM
QHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATEDEVSRFLAQAQPPTPAATP
SAPATGWQSLETSQNQADPVGPALNLFGGSTGLGQWSSSAGGSSGADLAGTSLWGPPNYSSSLWGVPTVE
DPHRMGSPAPLLPGDLLGGGSDSI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177124
ORF Size 5322 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177124.4, NP_796098.3
RefSeq Size 17125 bp
RefSeq ORF 5325 bp
Locus ID 213988
Cytogenetics 15 E1
Summary Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent translational repression and siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_177124" in other vectors (2)
SKU Description Size Price
MC205301 Tnrc6b (untagged) - Mouse trinucleotide repeat containing 6b (Tnrc6b), transcript variant 2, (10ug) 10 ug
$930.00
MR215989 Tnrc6b (Myc-DDK-tagged) - Mouse trinucleotide repeat containing 6b (Tnrc6b), transcript variant 2 10 ug
$1,640.00

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