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Zfp746 (NM_001163475) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215970
Zfp746 (tGFP-tagged) - Mouse zinc finger protein 746 (Zfp746), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $807.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Zfp746
Synonyms 2810407L07Rik; AI317225; Znf746
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215970 representing NM_001163475
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGGCGGCCGCGGCTCCGATTTCTCCATGGACAATGGCAGCTACAATTCAGGCTATGGAGAGGA
AGATTGAATCACAGGCTGCTCGACTGCTTTCTCTAGAAGGTCGAACTGGGATGGCAGAGAAGAAGCTGGC
TGACTGTGAGAAGACAGCTGTGGAGTTCAGCAACCAGCTGGAGGGCAAGTGGGCCGTGCTGGGAACCCTG
CTGCAGGAATATGGGCTGCTGCAGCGGCGGCTGGAAAATGTGGAGAATCTGCTACGCAACCGGAACTTCT
GGATTCTTCGGCTTCCCCCAGGCAGCAAGGGCGAGGTCCCCAAGGAGTGGGGCAAGCTGGAGGACTGGCA
GAAGGAGCTCTACAAGCATGTGATGAGGGGCAACTACGAGACACTGGTCTCCCTGGACTATGCCATCTCC
AAACCTGAGGTCCTCTCCCAGATTGAACAAGGCAAGGAGCCATGCACCTGGCGTCGCACTGGGCCCAAGG
TCCCGGAGGTTCCTGTGGACCCAAGTCCAGGCTCTGGAGCACCAGTTCCTGCCCCTGACCTCCTGATGCA
AATCAAGCAAGAGGGGGAGCTGCAGCTGCAGGAGCAGCAGGCTCTGGGTGTGGAAGCCTGGGCAGCTGGA
CAGCCAGATATTGGGGAAGAGCCGTGGGGCCTCAGCCAGTTGGACTCTGGAGCAGGAGACATCTCCACAG
ATGCTACCTCTGGTGTCCATTCCAACTTTTCAACCACCATCCCACCCACTTCCTGGCAAGCAGATCTCCC
TCCCCACCACCCCTCTTCAGCATGCTCAGATGGGACACTGAAGCTCAACACAGCAGCATCCACAGAAGCA
GATGTAAAAATTGTTATCAAAACAGAAGTCCAGGAAGAGGAGGTGGTGGCCACACCTGTTCATCCCACGG
ACCTGGAGGCTCATGGGACTTTGTTTGCACCAGGCCAAGCCACAAGATTCTTCCCTAGCCCTGTCCAGGA
AGGAGCCTGGGAAAGTCAAGGCAGTTCCTTCCCCAGCCAGGACCCAGTGCTAGGCCTGCGGGAGCCCACT
CGGCCAGAGAGGGACATTGGTGAGCTCAGTCCTGCCATAGCACAGGAGGAGGCTCCTGCCGGGGATTGGC
TCTTTGGCGGAGTCAGGTGGGGCTGGAATTTCCGGTGTAAACCTCCAGTGGGCCTGAACCCGAGGACGGT
GCCCGAGGGGCTCCCTTTCTCATCTCCAGACAACGGAGAGGCCATCTTGGACCCCAGCCAGGCCCCAAGA
CCCTTCAATGATCCCTGCAAATACCCTGGCCGGACAAAAGGCTTCGGCCACAAACCGGGGCTGAAGAAGC
ACCCGGCGGCACCACCCGGAGGCCGGCCCTTCACCTGTGCCACCTGCGGAAAGAGCTTCCAGCTGCAGGT
GAGCCTGAGTGCCCACCAGCGCAGCTGCGGACTGTCCGATGGGGCGGCCACGGGGGCAGCTTCCACCACT
ACTGGAGGCGGAGGTGGTGGCAGCGGCGGAGGAGGAGGCAGCAGCGGAGGTGGGAGCAGTGCACGGGACA
GCAGCGCTCTGAGGTGTGGGGAGTGTGGTCGCTGCTTCACACGCCCTGCACACCTCATCCGCCACCGCAT
GCTACACACTGGTGAGAGGCCCTTCCCCTGCACCGAGTGCGAGAAGCGCTTCACAGAGCGTTCCAAGCTC
ATCGACCACTACCGAACGCACACGGGCGTGCGGCCATTCACCTGCACAGTGTGTGGGAAGAGCTTCATCC
GCAAGGACCACCTCCGCAAGCACCAGCGCAACCACCCTGCAGTGGCCAAGGCGCCCGCACACGGTCAGCC
ACTCCCACCACTGCCGGCTCCTCCCGACCCTTTCAAGAGCCCCGCAGCCAAAGGACCCATGGCCTCCACA
GACCTTGTGACTGACTGGACTTGTGGCCTAAGTGTTCTGGGACCCAGTGATGGTGGTGGAGACCTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215970 representing NM_001163475
Red=Cloning site Green=Tags(s)

MAEAAAAPISPWTMAATIQAMERKIESQAARLLSLEGRTGMAEKKLADCEKTAVEFSNQLEGKWAVLGTL
LQEYGLLQRRLENVENLLRNRNFWILRLPPGSKGEVPKEWGKLEDWQKELYKHVMRGNYETLVSLDYAIS
KPEVLSQIEQGKEPCTWRRTGPKVPEVPVDPSPGSGAPVPAPDLLMQIKQEGELQLQEQQALGVEAWAAG
QPDIGEEPWGLSQLDSGAGDISTDATSGVHSNFSTTIPPTSWQADLPPHHPSSACSDGTLKLNTAASTEA
DVKIVIKTEVQEEEVVATPVHPTDLEAHGTLFAPGQATRFFPSPVQEGAWESQGSSFPSQDPVLGLREPT
RPERDIGELSPAIAQEEAPAGDWLFGGVRWGWNFRCKPPVGLNPRTVPEGLPFSSPDNGEAILDPSQAPR
PFNDPCKYPGRTKGFGHKPGLKKHPAAPPGGRPFTCATCGKSFQLQVSLSAHQRSCGLSDGAATGAASTT
TGGGGGGSGGGGGSSGGGSSARDSSALRCGECGRCFTRPAHLIRHRMLHTGERPFPCTECEKRFTERSKL
IDHYRTHTGVRPFTCTVCGKSFIRKDHLRKHQRNHPAVAKAPAHGQPLPPLPAPPDPFKSPAAKGPMAST
DLVTDWTCGLSVLGPSDGGGDL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001163475
ORF Size 1956 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163475.1, NP_001156947.1
RefSeq Size 3651 bp
RefSeq ORF 1959 bp
Locus ID 69228
UniProt ID Q3U133
Cytogenetics 6 B2.3
Summary Transcription repressor that specifically binds to the 5'-TATTTTT/G-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001163475" in other vectors (4)
SKU Description Size Price
MC220095 Zfp746 (untagged) - Mouse zinc finger protein 746 (Zfp746), (10ug) 10 ug
$669.00
MR215970 Zfp746 (Myc-DDK-tagged) - Mouse zinc finger protein 746 (Zfp746) 10 ug
$289.00 $607.00
MR215970L3 Lenti ORF clone of Zfp746 (Myc-DDK-tagged) - Mouse zinc finger protein 746 (Zfp746) 10 ug
$907.00
MR215970L4 Lenti ORF clone of Zfp746 (mGFP-tagged) - Mouse zinc finger protein 746 (Zfp746) 10 ug
$907.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.