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Sh3rf2 (NM_001146299) Mouse Tagged ORF Clone

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SKU
MG215957
Sh3rf2 (tGFP-tagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $874.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sh3rf2
Synonyms 2310046K19; 9130023G24Rik; AI452083; Ppp1r39; RNF158
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215957 representing NM_001146299
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGATTTGACGTTACTTGATCTCCTGGAGTGCCCTGTGTGCTTTGAAAAGCTCGATGTCACAGCCA
AAGTTCTCCCCTGCCAGCACACCTTCTGCAAACCATGTCTACAGAGGATTTTCAAGGCCCACAAGGAGCT
GAGGTGCCCGGAATGCAGGACTCTGGTGTTCTGCAGCATCGAGGCTCTGCCAGCCAACCTGTTGCTCGTG
CGTTTGCTGGATGGTGTGCGTTCCGGACAGAGCTCGTGGAAAGGAGGCTCCTTCCGAAGGCCTCGGATAC
TGACCTTGCAGGACAACAGGAAAGCCAAGAGCAGCCCCAGAAGTCTACAGGCTAGCCCTTTCCGGCTGGT
GCCCAGTGTCAGGATCCACATGGATGGGGTGCCTCGAGCCAAGGCCTTGTGCAACTACAGAGGGAAGAAT
CCTGGCGACCTGAAGTTTAATAAAGGCGATGTCATCCTCCTTCGGAGACAGCTTGATGAGAACTGGTACC
AGGGGGAGATTAATGGCGTCAGCGGGATCTTCCCGGCCAGCTCTGTGGAAGTCATCAAACAGCTGCCCCA
ACCACCCCCACTCTGCAGAGCCCTCTACAACTTTGACCTTCGAGACAAGGATAAGAGTGAGAACCAGGAC
TGCCTGACCTTCCTCAAGGATGATGTCATCACAGTGATCAGCCGTGTAGATGAGAACTGGGCAGAGGGAA
AGCTCGGAGATAAAGTGGGCATCTTCCCCATCTTGTTTGTAGAGCCAAACGTCTCGGCAAGACACCTCCT
AGAGAACAAAGGACATCAGTTATCACGCACAAGACACCTGTCCCTGATGTCCTCCCCATCCAGAGGCAAA
GCGACCAACACATCCTCGCTCCGAAAGAGCCCAGGGTCCAGGAGGAAGGGATCTGGGCAGTTCTCCATGA
CTACAGCTTTGAACACACTCAACCGTACAGTCCATTCTCCTGAAGGCCACCAGATGGTGGAAATCAGCAC
CCCGATGCTCATCAGCTCTAACAGCCCCTCTGTGCTCACCCAACATGGGGACAAGGCAGACTTCCCTACC
AGCTCTACAGGACAGGTCAGCAGTTCTCAGCCTGCACCTGCCTCCCCGGGACATTCCACAGCCATGGTCA
GTGTGCCCAGCTCCCAGCAACACCTTTCAAATAACATGTTTGTAGCCCTACACACTTACTCAGCCCATAG
ACCCGAAGAGCTGGACCTGCAGAAGGGAGAAGGCATCAGGGTTCTGGGCAAGTACCAGGATGGCTGGCTG
AAGGGCCTCTCCTTACTCACTGGACGAACTGGCATCTTTCCAAGCGACTATGTCATCCCAGTCTTCAGTT
CAACAGCTAGAAAGACATCTAGTTTTCCTGATTCCCGGAGCCCCACTGTCTGCACCACATGGGCGTTATC
CACCTCCTCTGTGTCCTCCCAGGGTAGCTTTGCAGAAGGTGATCCCCGGCAAAGTGGACCCTTCAAATCC
GTCTTTGTGCCCACTGCCGTAGTTAACCCTTCGAGGAGCACACCTGGCCCAGGGTCTTCTGGACAAGGGT
CTCTTAGGAAAGTGCGGAGCAGCATGAGAAAAAATGGATCCCTGCAGAGACCAGTTCAATCCGGGATCCC
CACTTTCATGGTGGGCTCCCTCAGGTGCAGTCCTACCATGGTGATCCGGCCTCAGAGGTTCCAGTTTTAT
CCGCCACAGGGGATGACCCCTTCCCCGACACCGATAATGGTGGAGATGGGGTCTAAATCTATTTACACTG
GGGAGCCTGCCCTGACCTGCATCAACAGAGGTAGCAAGACCAGGATCCACTCAGCAGGCAATTCCATCAT
CATGGAAGGCAAAGAAACGCCCATCAAGAGTGAACCACCACCCAAGCCGCCTGCATCTGCGCCACCCTCC
ATCCTCGTGAAACCAGAAAATTCAAAGAATGGCACTGAAAAGCAAGTCAAAACCGTGAGATTTCAGAATT
ACAGCCCTCCGCCCACCAAACACTCTGCCTCCAGTCCCACCTCTGGAAAGCACGACCATCCAGCCACCTT
GAAGGGGTCCCAGCATGAAGCAGTCTCCTCGGGAGGAGAGATGACCATTCTATTTGCCCACCGAAGTGGC
TGCCACTCCGGGCAACAAACAGACCTTCGGAGAAAGTCAGCCTTTGGCAAGACCATGCCCTTATTGTCCA
CTGCTTCAGCCACACAGACCTTGTTTCCCAGCAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215957 representing NM_001146299
Red=Cloning site Green=Tags(s)

MDDLTLLDLLECPVCFEKLDVTAKVLPCQHTFCKPCLQRIFKAHKELRCPECRTLVFCSIEALPANLLLV
RLLDGVRSGQSSWKGGSFRRPRILTLQDNRKAKSSPRSLQASPFRLVPSVRIHMDGVPRAKALCNYRGKN
PGDLKFNKGDVILLRRQLDENWYQGEINGVSGIFPASSVEVIKQLPQPPPLCRALYNFDLRDKDKSENQD
CLTFLKDDVITVISRVDENWAEGKLGDKVGIFPILFVEPNVSARHLLENKGHQLSRTRHLSLMSSPSRGK
ATNTSSLRKSPGSRRKGSGQFSMTTALNTLNRTVHSPEGHQMVEISTPMLISSNSPSVLTQHGDKADFPT
SSTGQVSSSQPAPASPGHSTAMVSVPSSQQHLSNNMFVALHTYSAHRPEELDLQKGEGIRVLGKYQDGWL
KGLSLLTGRTGIFPSDYVIPVFSSTARKTSSFPDSRSPTVCTTWALSTSSVSSQGSFAEGDPRQSGPFKS
VFVPTAVVNPSRSTPGPGSSGQGSLRKVRSSMRKNGSLQRPVQSGIPTFMVGSLRCSPTMVIRPQRFQFY
PPQGMTPSPTPIMVEMGSKSIYTGEPALTCINRGSKTRIHSAGNSIIMEGKETPIKSEPPPKPPASAPPS
ILVKPENSKNGTEKQVKTVRFQNYSPPPTKHSASSPTSGKHDHPATLKGSQHEAVSSGGEMTILFAHRSG
CHSGQQTDLRRKSAFGKTMPLLSTASATQTLFPSK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001146299
ORF Size 2205 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001146299.1, NP_001139771.1
RefSeq Size 4784 bp
RefSeq ORF 2208 bp
Locus ID 269016
UniProt ID Q8BZT2
Cytogenetics 18 B3
Summary Has E3 ubiquitin-protein ligase activity. Acts as an anti-apoptotic regulator of the JNK pathway by ubiquitinating and promoting the degradation of SH3RF1, a scaffold protein that is required for pro-apoptotic JNK activation. Facilitates TNF-alpha-mediated recruitment of adapter proteins TRADD and RIPK1 to TNFRSF1A and regulates PAK4 protein stability via inhibition of its ubiquitin-mediated proteasomal degradation. Inhibits PPP1CA phosphatase activity (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001146299" in other vectors (4)
SKU Description Size Price
MC221203 Sh3rf2 (untagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2), transcript variant 1, (10ug) 10 ug
$741.00
MR215957 Sh3rf2 (Myc-DDK-tagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2), transcript variant 1 10 ug
$589.00 $674.00
MR215957L3 Lenti ORF clone of Sh3rf2 (Myc-DDK-tagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2), transcript variant 1 10 ug
$974.00
MR215957L4 Lenti ORF clone of Sh3rf2 (mGFP-tagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2), transcript variant 1 10 ug
$974.00

file-alt Citations

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