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Gsg1 (NM_001080553) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215951
Gsg1 (tGFP-tagged) - Mouse germ cell-specific gene 1 (Gsg1) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
  $703.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gsg1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215951 representing NM_001080553
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTTCCAGAAGGGTTCCTCTGACCAGCGGACCTTCATTTCTGCCATCCTCAACATGTTATCACTCG
GCCTCTCCACAGCATCCTTGCTCAGCAGCGAGTGGTTTGTGGGCACACAGAAGGTGCCCAAGCCCCTGTG
CGGGCAAAGTCTGGCAGCCAAGTGCTTTGACATGCCGATGTCCTTGGATGGGGGCATCGCCAACACATCA
GCCCAGGAGGTGGTACAATACACCTGGGAGACTGGGGATGACCGGTTCTCCTTCCTTGCCTTCCGCAGTG
GCATGTGGCTATCCTGTGAGGAAACCATGGAAGAGCCAGGGGAGAAGTGCAGACGTTTCATTGAACTCAC
ACCACCAGCCCAGAGAGGTGAGAAAGGACTACTGGAATTTGCCACGTTGCAAGGCTCATGTCACCCCACT
CTCCGATTTGGAGGGGAGTGGTTAATGGAGAAGGCTTCTCTCCTCCACCTCCCTTGGGGGCCCGTGGCAA
AGGTCTTTTGGCTGTCACTGGGAGCCCAGACTGCCTATATCGGACTTCAACTCATCAGCTTCCTCCTGCT
ACTGACGGATCTGTTGCTCACCACGAACCCTGGCTGTGGGCTCAAGCTAAGCGCGTTTGCAGCCGTCTCC
TTGGTCCTGTCAGGACTTCTGGGGATGGTGGCTCATATGCTATATTCACAAGTCTTCCAGGCAACTGCCA
ACTTAGGTCCGGAGGACTGGAGACCACACTCTTGGAATTACGGCTGGGCCTTCTACACAGCGTGGGTTTC
CTTCACCTGCTGCATGGCGTCAGCGGTCACCACCTTCAACATGTACACGAGGATGGTGCTGGAGTTCAAG
TGCAGGCACAGCAAGAGCTTTAACACCAACCCCAGCTGCCTGGCGCAGCACCACCGCTGTTTCCTTCCTC
CTCCGCTGACGTGCACAACCCACGCAGGGGAACCTTTGTCCAGCTGCCATCAGTACCCCAGCCATCCCAT
CCGCTCTGTCTCTGAAGCTATTGACCTCTACTCGGCGCTACAGGACAAAGAATTTCAACAAGGGATCAGC
CAGGAGCTAAAGGAAGTGGTCGAGCCATCTGTAGAAGAGCAGCGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215951 representing NM_001080553
Red=Cloning site Green=Tags(s)

MEFQKGSSDQRTFISAILNMLSLGLSTASLLSSEWFVGTQKVPKPLCGQSLAAKCFDMPMSLDGGIANTS
AQEVVQYTWETGDDRFSFLAFRSGMWLSCEETMEEPGEKCRRFIELTPPAQRGEKGLLEFATLQGSCHPT
LRFGGEWLMEKASLLHLPWGPVAKVFWLSLGAQTAYIGLQLISFLLLLTDLLLTTNPGCGLKLSAFAAVS
LVLSGLLGMVAHMLYSQVFQATANLGPEDWRPHSWNYGWAFYTAWVSFTCCMASAVTTFNMYTRMVLEFK
CRHSKSFNTNPSCLAQHHRCFLPPPLTCTTHAGEPLSSCHQYPSHPIRSVSEAIDLYSALQDKEFQQGIS
QELKEVVEPSVEEQR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001080553
ORF Size 1095 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001080553.1, NP_001074022.1
RefSeq Size 1388 bp
RefSeq ORF 1098 bp
Locus ID 14840
UniProt ID Q8R1W2
Cytogenetics 6 66.07 cM
Summary May cause the redistribution of PAPOLB from the cytosol to the endoplasmic reticulum.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001080553" in other vectors (4)
SKU Description Size Price
MC202183 Gsg1 (untagged) - Mouse germ cell-specific gene 1 (Gsg1), transcript variant 2, (10ug) 10 ug
$457.00
MR215951 Gsg1 (Myc-DDK-tagged) - Mouse germ cell-specific gene 1 (Gsg1), transcript variant 2 10 ug
$503.00
MR215951L3 Lenti ORF clone of Gsg1 (Myc-DDK-tagged) - Mouse germ cell-specific gene 1 (Gsg1), transcript variant 2 10 ug
$803.00
MR215951L4 Lenti ORF clone of Gsg1 (mGFP-tagged) - Mouse germ cell-specific gene 1 (Gsg1), transcript variant 2 10 ug
$803.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.