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Ndor1 (NM_001082476) Mouse Tagged ORF Clone

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SKU
MG215946
Ndor1 (tGFP-tagged) - Mouse NADPH dependent diflavin oxidoreductase 1 (Ndor1), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $757.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ndor1
Synonyms 4930447P04Rik; Ndor; NR1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215946 representing NM_001082476
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGTTCCACAGCTCCTGGTGCTTTTCGGCAGCCAGACTGGTACAGCCCAGGACGAGGCGGAGAGGC
TGGGCCGTGAGGCCCGGCGCCGGCGGCTAGGTTGCCGCGTGCAGGCTTTGGACTCTTACTCCGTGGCGAA
TCTCATTAGGGAGCCCCTGGTGATATTTGTTTGTGCGACCACAGGCCAAGGAGACCCTCCTGACAACATG
AAGAACTTCTGGAGGTTCATATTCCGGAAGAGCCTGCCGTCCAGCTCCCTCTGTCAAATGGATTTTGCTG
TCCTAGGCCTCGGGGATTCCTCATATGCCAAGTTCAACTTTGTGGCCAAGAAGCTACATCGTAGGCTGCT
GCAGCTAGGAGGCAGTGCGCTTCTGCCCCCATGTCTGGGTGATGACCAGCATGAGCTGGGGCCTGATGCT
GCCATTGACCCCTGGGTAGGAGATCTTTGGGAGAAGATAATGGTTATGTACCCAGTACCCCTTGACATTC
CTGAGATTCCTCATGGAGTCCCCTTGCCCTCCAAGTTCATCTTTCAGTTTCTTCAGGAGGTCCCCAGCAT
AGGGGCTGAGGAGCTCAACATAGCCAGCTCAGCCCCTCAGACACCCCCATCAGAGTTACAGCCCTTCCTG
GCACCTGTGATCACCAACCAGAGGGTCACTGGCCCCCAACACTTTCAGGATGTTCGGCTGATTGAATTTG
ACATTACAGACTCCAATATCAGCTTTGCTGCTGGTGATGTTGTATTCATTTTGCCCTCTAACTCGGAGGC
CCACACCCAGCAGTTCTGCCAAGTGTTGTGCCTGGACCCCAATCAGTTTTTCACACTTAAGCCACGGGAA
CCAGGTGTCCCTGACCCACCAGGGCTGCCTCAGCCCTGTACTGTGTGGAACCTCGTGTCCCAGTACTTGG
ACATTGCCAGTGTGCCCCGCCGTTCCTTCTTTGAGCTCCTGGCTTGTCTCTCCCAACATGCGCTGGAGCG
AGAGAAGCTGCTGGAATTGAGCTCTGCCAGAGGTCAGGAAGAGCTCTGGGAGTACTGCAGTCGGCCCCGC
AGAACCATCTTGGAGGTGCTATGCGACTTTCCACATACAGCGGGTGCCATTCCTCCAGACTACCTTTTGG
ACCTAATCCCTCGGATCCGCCCACGGGCCTTCTCCATTGCTTCCTCCCTACTGGCTCATCCCAGGAGGCT
ACAGATCCTTGTGGCTGTGGTAAAGTACCAGACTCGTCTGAAGGAGCCCCGCCATGGTCTCTGCTCCTCC
TGGCTAGCATCCCTGAATCCTGGGCAAGCAGGACCTGTCCGGGTGCCTCTGTGGGTACGTCCTGGGAGCC
TCGTGTTCCCAAAAACACCAGACACACCCATTATCATGGTGGGGGCTGGAACTGGTGTGGCCCCCTTCCG
AGCAGCCATTCAGGAACGAGTGGCTCATGGCCAAACTGGAAACTTCTTGTTCTTTGGCTGCCGCCAGCGG
GATCAGGACTTCTACTGGCAGACTGAATGGCAGAAGCTAGAGCAGAAGGGCTGGCTGACCCTGGTTACAG
CCTTCTCCCGGGAGCAGGAGCAGAAGGTATATGTGCAGCACCGGCTTCGAGAGCTGGGGCCACTTGTCTG
GGAGCTGCTGGATGGCCAAGGTGCCTACTTCTACCTAGCAGGCAATGCTAAATATTTGCCTACGGATGTT
TCGGAAGCCCTTATGTCCATTTTTCAAGAAGAAGGACGACTCTCCACTGCAGATGCATCTGCCTACCTTG
CTAGGCTCCAGCAGACACTGCGCTTCCAGACTGAAACCTGGGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215946 representing NM_001082476
Red=Cloning site Green=Tags(s)

MQVPQLLVLFGSQTGTAQDEAERLGREARRRRLGCRVQALDSYSVANLIREPLVIFVCATTGQGDPPDNM
KNFWRFIFRKSLPSSSLCQMDFAVLGLGDSSYAKFNFVAKKLHRRLLQLGGSALLPPCLGDDQHELGPDA
AIDPWVGDLWEKIMVMYPVPLDIPEIPHGVPLPSKFIFQFLQEVPSIGAEELNIASSAPQTPPSELQPFL
APVITNQRVTGPQHFQDVRLIEFDITDSNISFAAGDVVFILPSNSEAHTQQFCQVLCLDPNQFFTLKPRE
PGVPDPPGLPQPCTVWNLVSQYLDIASVPRRSFFELLACLSQHALEREKLLELSSARGQEELWEYCSRPR
RTILEVLCDFPHTAGAIPPDYLLDLIPRIRPRAFSIASSLLAHPRRLQILVAVVKYQTRLKEPRHGLCSS
WLASLNPGQAGPVRVPLWVRPGSLVFPKTPDTPIIMVGAGTGVAPFRAAIQERVAHGQTGNFLFFGCRQR
DQDFYWQTEWQKLEQKGWLTLVTAFSREQEQKVYVQHRLRELGPLVWELLDGQGAYFYLAGNAKYLPTDV
SEALMSIFQEEGRLSTADASAYLARLQQTLRFQTETWA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001082476
ORF Size 1794 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001082476.2, NP_001075945.1
RefSeq Size 4757 bp
RefSeq ORF 1797 bp
Locus ID 78797
UniProt ID A2AI05
Cytogenetics 2 A3
Summary Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Transfers electrons from NADPH to the Fe/S cluster of CIAPIN1.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001082476" in other vectors (3)
SKU Description Size Price
MR215946 Ndor1 (Myc-DDK-tagged) - Mouse NADPH dependent diflavin oxidoreductase 1 (Ndor1) 10 ug
$289.00 $557.00
MR215946L3 Lenti ORF clone of Ndor1 (Myc-DDK-tagged) - Mouse NADPH dependent diflavin oxidoreductase 1 (Ndor1) 10 ug
$857.00
MR215946L4 Lenti ORF clone of Ndor1 (mGFP-tagged) - Mouse NADPH dependent diflavin oxidoreductase 1 (Ndor1) 10 ug
$857.00

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