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Qtrt1 (NM_021888) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215941
Qtrt1 (tGFP-tagged) - Mouse queuine tRNA-ribosyltransferase 1 (Qtrt1), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Qtrt1
Synonyms 2610028E17Rik; Tgt
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215941 representing NM_021888
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGTAGGCAGCCCAGGTTCGCTTGAGTCCGCTCCACGAATAATGCGGCTGGTCGCTGAGTGCA
GTCGCTCCGGAGCTCGGGCGGGTGAGCTGCGGCTGCCGCATGGAACGGTAGCCACCCCTGTGTTCATGCC
TGTGGGTACACAGGCCACCATGAAGGGTATCACCACGGAGCAGCTGGACTCCCTGGGCTGCCGCATCTGC
TTGGGCAACACCTACCATCTGGGGCTGAGGCCGGGACCGGAGCTGATCCGGAAAGCCCAGGGTCTTCACG
GCTTCATGAATTGGCCCCACAATCTGCTGACGGATAGCGGCGGCTTCCAGATGGTGTCCCTCTTCTCCCT
GTCCGAGGTGACGGAGGAGGGCGTCCACTTCCGCTCGCCCTACGATGGAGAAGAGACACTTTTGAGCCCA
GAGAGGTCGGTGGAGATCCAGAATGCTCTGGGCTCGGACATCATCATGCAGTTAGACCATGTGGTGAGCA
GCACAGTGACAGGCCCTCTAGTGGAGGAGGCCATGCACAGGTCAGTCCGCTGGCTGGACAGATGCATCGC
AGCCCATAAGCACCCGGACAAGCAGAACCTCTTTGCCATCATCCAGGGTGGACTGAATGCTGATCTTCGG
ACCACTTGTCTGAAAGAGATGACCAAGAGAGATGTGCCGGGCTTCGCCATCGGAGGTCTGAGTGGAGGAG
AAAGCAAGGCACAGTTCTGGAAGATGGTGGCGTTGAGCACCTCCATGCTGCCTAAGGACAAGCCACGATA
CCTGATGGGGGTTGGCTATGCCACCGATTTGGTGGTCTGCGTGGCTCTTGGATGTGACATGTTCGACTGT
GTGTACCCCACACGGACAGCGCGCTTTGGATCCGCCCTTGTGCCCACTGGGAACCTTCAGTTGAAGAAGA
AGCAGTATGCCAAGGATTTCAGCCCCATAAACCCTGAGTGTCCCTGTCCCACCTGCCAGACGCACAGCCG
AGCCTTCCTGCATGCCCTGCTACACAGTGACAACACTACAGCACTACACCACCTAACCGTGCACAACATT
GCCTATCAGCTGCAGCTCCTGAGTGCCGTGCGCAGCAGTATCTTGGAACAGCGCTTTCCTGACTTTGTAC
GAAACTTCATGCGCACTATGTATGGTGACCACAGCCTCTGTCCTGCCTGGGCCGTTGAAGCACTGGCCTC
TGTGGGAATCATGCTCACA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215941 representing NM_021888
Red=Cloning site Green=Tags(s)

MAAVGSPGSLESAPRIMRLVAECSRSGARAGELRLPHGTVATPVFMPVGTQATMKGITTEQLDSLGCRIC
LGNTYHLGLRPGPELIRKAQGLHGFMNWPHNLLTDSGGFQMVSLFSLSEVTEEGVHFRSPYDGEETLLSP
ERSVEIQNALGSDIIMQLDHVVSSTVTGPLVEEAMHRSVRWLDRCIAAHKHPDKQNLFAIIQGGLNADLR
TTCLKEMTKRDVPGFAIGGLSGGESKAQFWKMVALSTSMLPKDKPRYLMGVGYATDLVVCVALGCDMFDC
VYPTRTARFGSALVPTGNLQLKKKQYAKDFSPINPECPCPTCQTHSRAFLHALLHSDNTTALHHLTVHNI
AYQLQLLSAVRSSILEQRFPDFVRNFMRTMYGDHSLCPAWAVEALASVGIMLT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021888
ORF Size 1209 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021888.2, NP_068688.2
RefSeq Size 1328 bp
RefSeq ORF 1212 bp
Locus ID 60507
UniProt ID Q9JMA2
Cytogenetics 9 A3
Summary Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:19414587, PubMed:29862811). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_021888" in other vectors (4)
SKU Description Size Price
MC210209 Qtrt1 (untagged) - Mouse queuine tRNA-ribosyltransferase 1 (Qtrt1), (10ug) 10 ug
$503.00
MR215941 Qtrt1 (Myc-DDK-tagged) - Mouse queuine tRNA-ribosyltransferase 1 (Qtrt1) 10 ug
$289.00 $457.00
MR215941L3 Lenti ORF clone of Qtrt1 (Myc-DDK-tagged) - Mouse queuine tRNA-ribosyltransferase 1 (Qtrt1) 10 ug
$757.00
MR215941L4 Lenti ORF clone of Qtrt1 (mGFP-tagged) - Mouse queuine tRNA-ribosyltransferase 1 (Qtrt1) 10 ug
$757.00

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