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Efhc2 (NM_028916) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215934
Efhc2 (tGFP-tagged) - Mouse EF-hand domain (C-terminal) containing 2 (Efhc2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $887.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Efhc2
Synonyms 4933407D04Rik; mRib72-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215934 representing NM_028916
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTACCGTTTCTGCCTGGCAACAGCTTTAACCGCAATATAGGAAAGGAGAGGTTCCATAAATCTC
AACATTGGGGCTTTTGCAACAATGTCAGGATGCTGGTGAGTGAGAATAAGCCTGGAGTAGGTGGAGATCT
GCTATATGGCCAAAAGATAAAGCCTAAACACAGTGTATTTCCCAAAGGAGATGGAACCGATGCACCATCC
TGGGTTGCTTTTGATAAGCAGGTGTTATCTTTTGATGCCTATTTGGAAGATGAAATATCTGATAAAAGAC
AAGAAATCTTTAGAATAAGATACTACAAAATCTATTTCTACCTTGAAGATGACACCATTCAAGTAAATGA
ACCAGAAGTGATAAATAGTGGACTGCCTCAAGGCACTTCTATCAGGCGTCAACGGATTCCTTACCCCCCT
CCCAATGATGATCAGTTTTATACTGTATATGACTTCAATATCAACATAAGTGTTGTCTTCTATGGGCGCA
CATTCAAGATTTATGATTGCGATCCATTTACAAAAAACTTTTTGAAGAAAATAGGAATCAAATTGAACCC
TCCAGGACAATGTCCACTCGATCCCTACATGAAGATGCGGAGAGAGACATTAGAGTTTGTGGATCCCTTT
CGTCCCTATCAGTCTTTTGACACCCTGAAACGATTCATCCAGTATGATGGGAAGGTTTTGCGTTTCTTCT
GCTTGTGGGATGATTCAACCTCACTGTTTGGGGACCGTAGAGAATTTGTCCTGCATTACTTCCTGTGTGA
TGGTACTGTTGAAATCAGGGAAGTACTGCCATCTAATTCAGGCCGGGATGCTATGTCATCATTCCTTCGG
CGGGGTAAGCTTCCCAAGTATGGCCCACCTGGAATCTACCAACCAGGTCAGATAACAGATCGAGCAGTTC
TCAATGTGTATGGAGGCTTGTCAGAATGGCGAGCAGATGGCTATCTGCTGGATAAATACCAGCTAGGAAA
AGTAGAGCAAGACTTTTACACAGACCAGGACCTGTCTATAGGTGCCACCATCAATGTATGGGGAAGAAAA
GTCCTCCTCTGTGACTGTGATGAGTTTACCAAGACCTATTATAGGACAAAGTATGGAGTTGATAACTTTA
CGCCCATTTCATGCAAGCCTCCTCATCTTCCAAAGATAGAAAGGAAATACCCACCTTATACTGGCTTTGG
GTCTGAAGAGGATTCTTTCCGCTCCTGTGTAGGCCTGAAGCCCACACCTCATCGGAAGAACTTCAAAAAA
TTCATGGAACTAGACAGCTTTGGAAACATAAGCAATATACTCCGATATTTTGGAAAACTAATTACACACA
AATGCGCTGATGTGGACAGGATCTTTGTCATTGCATTTTATCTCAGTGATGACACAATTTCAGTGTTTGA
GCCTATAGAGAATAATTCAGGGAATGCTGGTGGAATGTTCTTAAAAAGAAGTCGTGTGAAAAAGCCTGGA
CAAGAAGTTTTTAAAAGTGAATTTTCAGAATACATCAAGGCTGAAGAGCTCTATATTGGAGCCACAGTGA
ATATTAATGGCTACCTGTTTATTCTGCTTAATGCTGATGAGTACACCTTAAACTACATGGAGAATAACAC
AGACAAGTTTCCATATAGCAACTTTGAACTTGCCATACAAAAACTGAAGCAGGAAAAATCAAAATCAAGA
GAGATCACACAAGTATTTGCGGCTGCTGACTATAATCACACGAAGGTGGTACCTTACAATACATTCAGAG
ATATCCTGATGAGTATCACCATGGGGAAGCTCATAGACCAGGAGCTTATAACCATTGCACGCCACTACCG
TGTGCCTGAGATTATGGATCCAGACTTAGCATACTTAATTGCCCGGGCCCATGAAAAGTTCAAGAAAAAT
ATATTTGAGAATTTTGACATGTTCATTTATAACTGTGTGTATGAAGACAGAGAAAAAAAAGGTGTATTAC
CCACCAAAGACATCAGAAGGATGTGCAAGTCTTCCAGGCTACCTCTGGATGATGACTTTCTAGATTGCTT
ACTGTCCAGGTTTGAAGACAAAGACCATCAAATAAATTATGAGATATTTTTCTCCGTCCTGAACTGGAGA
ATGAATCCAACACCGGATTTGCAAGCCCCGCCATACCTTAAAGAGAAATGTGAAGACGTTTGGGTGGGAA
TGCCATCACCTATTCCTGTGAAATACGTTAGATACTTAGACTTTTTGATTGATGTATATGGCTTAGAGGA
CAACATGCTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215934 representing NM_028916
Red=Cloning site Green=Tags(s)

MALPFLPGNSFNRNIGKERFHKSQHWGFCNNVRMLVSENKPGVGGDLLYGQKIKPKHSVFPKGDGTDAPS
WVAFDKQVLSFDAYLEDEISDKRQEIFRIRYYKIYFYLEDDTIQVNEPEVINSGLPQGTSIRRQRIPYPP
PNDDQFYTVYDFNINISVVFYGRTFKIYDCDPFTKNFLKKIGIKLNPPGQCPLDPYMKMRRETLEFVDPF
RPYQSFDTLKRFIQYDGKVLRFFCLWDDSTSLFGDRREFVLHYFLCDGTVEIREVLPSNSGRDAMSSFLR
RGKLPKYGPPGIYQPGQITDRAVLNVYGGLSEWRADGYLLDKYQLGKVEQDFYTDQDLSIGATINVWGRK
VLLCDCDEFTKTYYRTKYGVDNFTPISCKPPHLPKIERKYPPYTGFGSEEDSFRSCVGLKPTPHRKNFKK
FMELDSFGNISNILRYFGKLITHKCADVDRIFVIAFYLSDDTISVFEPIENNSGNAGGMFLKRSRVKKPG
QEVFKSEFSEYIKAEELYIGATVNINGYLFILLNADEYTLNYMENNTDKFPYSNFELAIQKLKQEKSKSR
EITQVFAAADYNHTKVVPYNTFRDILMSITMGKLIDQELITIARHYRVPEIMDPDLAYLIARAHEKFKKN
IFENFDMFIYNCVYEDREKKGVLPTKDIRRMCKSSRLPLDDDFLDCLLSRFEDKDHQINYEIFFSVLNWR
MNPTPDLQAPPYLKEKCEDVWVGMPSPIPVKYVRYLDFLIDVYGLEDNML

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028916
ORF Size 2250 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028916.5
RefSeq Size 2570 bp
RefSeq ORF 2253 bp
Locus ID 74405
UniProt ID Q9D485
Cytogenetics X A1.2
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Documents
Resources
"NM_028916" in other vectors (4)
SKU Description Size Price
MC221312 Efhc2 (untagged) - Mouse EF-hand domain (C-terminal) containing 2 (Efhc2), (10ug) 10 ug
$756.00
MR215934 Efhc2 (Myc-DDK-tagged) - Mouse EF-hand domain (C-terminal) containing 2 (Efhc2) 10 ug
$589.00 $687.00
MR215934L3 Lenti ORF clone of Efhc2 (Myc-DDK-tagged) - Mouse EF-hand domain (C-terminal) containing 2 (Efhc2) 10 ug
$987.00
MR215934L4 Lenti ORF clone of Efhc2 (mGFP-tagged) - Mouse EF-hand domain (C-terminal) containing 2 (Efhc2) 10 ug
$987.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.