March1 (NM_001166375) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG215930
41334 (tGFP-tagged) - Mouse membrane-associated ring finger (C3HC4) 1 (March1) transcript variant 2, (10ug)
$489.00 $530.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol March1
Synonyms 2900024D24Rik; BB085186
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001166375
ORF Size 825 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001166375.1, NP_001159847.1
RefSeq Size 3942 bp
RefSeq ORF 828 bp
Locus ID 72925
UniProt ID Q6NZQ8
Cytogenetics 8 B3.1
Summary E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001166375" in other vectors (3)
SKU Description Size Price
MR215930 41334 (Myc-DDK-tagged) - Mouse membrane-associated ring finger (C3HC4) 1 (March1), transcript variant 2 10 ug
$330.00
MR215930L3 Lenti ORF clone of 41334 (Myc-DDK-tagged) - Mouse membrane-associated ring finger (C3HC4) 1 (March1), transcript variant 2 10 ug
$630.00
MR215930L4 Lenti ORF clone of 41334 (mGFP-tagged) - Mouse membrane-associated ring finger (C3HC4) 1 (March1), transcript variant 2 10 ug
$630.00

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