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Afg1l (NM_145743) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215922
Lace1 (tGFP-tagged) - Mouse lactation elevated 1 (Lace1), (10ug)
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ORF Plasmid:
tGFP DDK
  $886.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Afg1l
Synonyms CG8520; Lace1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215922 representing NM_145743
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCTCCTGGTCGCCCTTGGTTACTCTGCGCTCCGCCGCGCGCAGCCGGCTGACTGGGAGAGGTG
TTGGGTGCGGGGCGCGGGTCGTCGCAATCCCTCCTCCGGCCCCTGGCCCTGGGAAACCCCTGTGGAAAGC
CTACACAGTCCAGACATCCGAGGGTGTACGCCCAACAGCCGCTTCTGAGGCCCGTTTGAAAGCCTTGGCT
GTTTGCCACGGGCCTCTGGACCACTACGACTTTCTGATCAAATCTCAGGAGCTGAGGGAGGATGAACATC
AAAGAAGAGTCGTTCAGTGTTTGCAGAAGTTACAGGAGGACCTTAAAGGATACAGCATAGAGGAAGGAGG
GCTCTTTTCAAAGCTTTTTTCAAGGAACAAACCTCCAAAGGGCCTCTATGTTTATGGAGACGTTGGTACA
GGAAAAACAATGGTGATGGACATGTTTTATGCATATGTGGAAACGAAGAGAAAGAAGCGGGTCCATTTTC
ACGGCTTCATGCTGGACGTGCACAGAAGGATACACCACCTTAAACAGAGTTTGCCAAAAAGGAAGGCGGG
ATTCATGGCTAAGTCATATGACCCGATAGCTCCCATAGCCGAGGAAATCAGTCAAGAAACTTCTCTCCTA
TGTTTTGATGAATTTCAGGTCACTGACATCGCCGACGCCATGATTCTGAAACAGCTTTTTGAAAATCTGT
TCAAAAACGGGGTCGTCGTTGTGGCAACATCCAACAGACCACCGGAAGATCTCTATAAAAATGGACTCCA
GAGGGCTAACTTTGTGCCATTTATAGCTGTCCTGAAGGAATATTGTGATACGCTCCAGCTGGATTCTGGG
GTAGATTACCGGAAAAGGGAACTCGCCCCTGCAGGAAAACTCTACTACCTCACAAGTGAGGCTGATGTGG
AGGCCGTCGTGGATAAGTTGTTTGATGAGCTGGCTCAGAAACAAAATGATTTAACTAGCCCAAGGATTCT
GAAAATGCAAGGCAGAGAGCTGCGGCTGAATAAAGCCTGTGGAAGCGTTGCCGACTGCACATTTGAAGAG
CTGTGTGAGAGACCACTTGGAGCAAGTGACTATTTGGAATTATCAAAGAATTTTGATACGGTAATCATAC
GAAACATTCCGCAGTTTTCACTGGCAAAGAGGACGCAAGCGAGAAGATTCATCACTCTCATTGATAACTT
CTATGACTTCAAGGTGCGCATTATCTGCTCCGCTTCAGCTCCGATCTCAAGCTTGTTTCTGCATCAGCAT
CAGGACAGTGAGTCGGACCAAAGCAGAATCCTGATGGATGATTTGGGGCTGAGCCAGGACTCAGCCGGGC
TCTCCATGTTTACGGGGGAGGAGGAAATCTTCGCCTTTCAGCGCACCATTTCCCGGCTCACAGAGATGCA
GACGGAGCAGTACTGGATTGAAGGGGACAGAAGCAGGAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215922 representing NM_145743
Red=Cloning site Green=Tags(s)

MAASWSPLVTLRSAARSRLTGRGVGCGARVVAIPPPAPGPGKPLWKAYTVQTSEGVRPTAASEARLKALA
VCHGPLDHYDFLIKSQELREDEHQRRVVQCLQKLQEDLKGYSIEEGGLFSKLFSRNKPPKGLYVYGDVGT
GKTMVMDMFYAYVETKRKKRVHFHGFMLDVHRRIHHLKQSLPKRKAGFMAKSYDPIAPIAEEISQETSLL
CFDEFQVTDIADAMILKQLFENLFKNGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCDTLQLDSG
VDYRKRELAPAGKLYYLTSEADVEAVVDKLFDELAQKQNDLTSPRILKMQGRELRLNKACGSVADCTFEE
LCERPLGASDYLELSKNFDTVIIRNIPQFSLAKRTQARRFITLIDNFYDFKVRIICSASAPISSLFLHQH
QDSESDQSRILMDDLGLSQDSAGLSMFTGEEEIFAFQRTISRLTEMQTEQYWIEGDRSRK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145743
ORF Size 1440 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145743.2, NP_665686.2
RefSeq Size 2530 bp
RefSeq ORF 1443 bp
Locus ID 215951
UniProt ID Q3V384
Cytogenetics 10 22.88 cM
Summary Putative mitochondrial ATPase. Plays a role in mitochondrial morphology and mitochondrial protein metabolism. Promotes degradation of excess nuclear-encoded complex IV subunits (COX4I1, COX5A and COX6A1) and normal activity of complexes III and IV of the respiratory chain. Mediates mitochondrial translocation of TP53 and its transcription-independent apoptosis in response to genotoxic stress.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_145743" in other vectors (4)
SKU Description Size Price
MC216712 Lace1 (untagged) - Mouse lactation elevated 1 (Lace1), (10ug) 10 ug
$503.00
MR215922 Lace1 (Myc-DDK-tagged) - Mouse lactation elevated 1 (Lace1) 10 ug
$686.00
MR215922L3 Lenti ORF clone of Lace1 (Myc-DDK-tagged) - Mouse lactation elevated 1 (Lace1) 10 ug
$986.00
MR215922L4 Lenti ORF clone of Lace1 (mGFP-tagged) - Mouse lactation elevated 1 (Lace1) 10 ug
$986.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.