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Akap3 (NM_009650) Mouse Tagged ORF Clone

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SKU
MG215888
Akap3 (tGFP-tagged) - Mouse A kinase (PRKA) anchor protein 3 (Akap3), (10ug)
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ORF Plasmid:
tGFP DDK
  $991.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Akap3
Synonyms Prka3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215888 representing NM_009650
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGATAGGGTTGACTGGTTACAAAGTCAAAGTGGCGTTTGCAAAGTTGGTGTCTATTCACCTGGAG
ACAACCAACACCAAGACTGGAAAATGGACACATCAACAGATCCTGTCCGAGTGCTCAGCTGGCTCCGCAA
AGACCTGGAGAAAAGTACAGCGGGCTTCCAGGACTCGAGGTTCAAGCCTGGAGAGTCATCGTTTGTGGAG
GAAGTGGCTTACCCAGTGGACCAACGAAAAGGTTTCTGTGTTGATTATTACAATACCACCAACAAGGGCA
GTCCAGGAAGATTGCATTTTGAGATGTCTCACAAGGAGAACCCTTCCCAGGGCCTCATTTCCCATGTTGG
TAATGGGGGTTCCATAGACGAAGTTTCCTTCTATGCCAACCGCCTCACAAACCTAGTGATCGCCATGGCC
CGAAAGGAGATCAATGAGAAGATCCACGGCGCTGAAAACAAATGTGTCCATCAGTCATTGTATATGGGGG
ATGAGCCCACACCCCACAAAAGCTTGAGTACAGTGGCCTCTGAGCTCGTGAACGAGACAGTCACCGCATG
TTCCAAGAACATTTCCAGTGACAAAGCTCCCGGCTCTGGAGACAGGGCCTCGGGGTCGTCACAGGCCCCT
GGTCTAAGATACACGAGCACTCTGAAGATCAAGGAGAGTACAAAGGAAGGCAAGTGTCCAGACGACAAGC
CCGGCACTAAGAAGTCTTTCTTCTATAAGGAAGTGTTTGAGTCCCGGAATGCAGGAGATGCCAAGGAGGG
CGGGAGGTCCTTACCAGGAGATCAAAAACTGTTCAGGACCAGCCCCGACAACAGGCCTGATGACTTTTCA
AACTCTATCAGTCAAGGGATCATGACCTACGCCAACAGCGTGGTGTCTGACATGATGGTCTCCATCATGA
AGACGCTGAAGATCCAGGTGAAGGACACAACCATCGCCACGATTCTGCTGAAGAAGGTGCTGATGAAGCA
CGCAAAGGAGGTTGTCTCCGACCTCATCGACTCCTTCATGAAGAACCTCCACGGCGTCACGGGAAGCCTC
ATGACTGACACAGACTTTGTCTCGGCCGTGAAACGAAGTTTTTTTTCTCATGGAAGCCAAAAGGCCACAG
ATATCATGGATGCCATGCTGGGCAAGCTATACAATGTGATGTTTGCCAAGAAATTCCCTGAGAACATCCG
GAGAGCCAGGGACAAGTCGGAGAGTTACTCCCTTATCTCCACGAAATCACGGGCTGGTGACCCAAAGCTC
TCAAATTTGAACTTTGCGATGAAGTCAGAATCAAAGCTGAAAGAAAATTTGTTTTCTACATGCAAACTAG
AGAAAGAGAAGACGTGTGCCGAAACTCTGGGTGAGCATATTATTAAGGAGGGACTGCACATGTGGCACAA
GAGTCAGCAAAAATCTCCTGGCTTGGAGCGTGCCGCAAAACTGGGTAACGCTCCACAGGAGGTCTCCTTT
GAGTGCCCAGATCCTTGTGAGGCAAACCCTCCTCACCAACCTCAGCCACCAGAGAATTTTGCAAATTTTA
TGTGTGACTCAGACTCCTGGGCCAAGGACCTGATTGTATCCGCCCTGCTTCTGATTCAGTATCACCTGGC
ACAGGGAGGAAAGATGGATGCTCAGAGCTTCCTGGAAGCTGCTGCCAGCACCAATTTTCCCACCAACAAG
CCACCTCCTCCTTCTCCTGTAGTTCAGGATGAGTGCAAACTTAAGTCTCCTCCCCACAAGATATGTGACC
AAGAACAAACAGAAAAGAAAGATCTGATGAGTGTCATCTTCAATTTTATCCGGAACTTACTCAGCGAGAC
CATATTCAAGAGTAGCCGTAACTGTGAATCCAATGTGCATGAGCAGAACACTCAGGAAGAAGAGATACAC
CCGTGTGAAAGGCCTAAGACTCCATGTGAAAGGCCTATTACCCCGCCTGCCCCGAAATTCTGTGAGGATG
AGGAGGCCACTGGTGGTGCCTTATCTGGGCTAACCAAGATGGTTGCCAACCAGCTAGACAACTGTATGAA
TGGGCAGATGGTGGAGCACCTGATGGACTCGGTGATGAAGTTATGCCTCATTATTGCCAAGTCCTGTGAC
TCTCCCCTGTCGGAGCTGGGAGAGGAAAAGTGTGGGGATGCCAGCCGGCCAAATTCTGCCTTCCCAGATA
ACTTATATGAGTGCCTACCAGTCAAGGGCACAGGGACAGCTGAAGCCCTCCTGCAGAACGCCTACCTCAC
CATCCATAATGAACTGAGAGGTTTGTCAGGACAGCCCCCCGAGGGCTGTGAAATCCCCAAGGTGATCGTC
AGCAACCACAATCTGGCTGACACCGTTCAGAACAAGCAACTGCAAGCTGTCCTTCAGTGGGTGGCTGCCT
CAGAGCTCAATGTCCCTATTTTGTACTTTGCTGGTGACGATGAAGGAATCCAGGAGAAGCTGCTTCAGCT
CTCAGCCACTGCCGTGGAGAAAGGCCGCAGCGTCGGGGAGGTTCTGCAGTCGGTGCTGAGGTACGAGAAG
GAGCGACAGCTGGATGAAGCAGTGGGAAATGTCACGCGGCTGCAGCTGCTGGACTGGCTGATGGCAAACC
TG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215888 representing NM_009650
Red=Cloning site Green=Tags(s)

MADRVDWLQSQSGVCKVGVYSPGDNQHQDWKMDTSTDPVRVLSWLRKDLEKSTAGFQDSRFKPGESSFVE
EVAYPVDQRKGFCVDYYNTTNKGSPGRLHFEMSHKENPSQGLISHVGNGGSIDEVSFYANRLTNLVIAMA
RKEINEKIHGAENKCVHQSLYMGDEPTPHKSLSTVASELVNETVTACSKNISSDKAPGSGDRASGSSQAP
GLRYTSTLKIKESTKEGKCPDDKPGTKKSFFYKEVFESRNAGDAKEGGRSLPGDQKLFRTSPDNRPDDFS
NSISQGIMTYANSVVSDMMVSIMKTLKIQVKDTTIATILLKKVLMKHAKEVVSDLIDSFMKNLHGVTGSL
MTDTDFVSAVKRSFFSHGSQKATDIMDAMLGKLYNVMFAKKFPENIRRARDKSESYSLISTKSRAGDPKL
SNLNFAMKSESKLKENLFSTCKLEKEKTCAETLGEHIIKEGLHMWHKSQQKSPGLERAAKLGNAPQEVSF
ECPDPCEANPPHQPQPPENFANFMCDSDSWAKDLIVSALLLIQYHLAQGGKMDAQSFLEAAASTNFPTNK
PPPPSPVVQDECKLKSPPHKICDQEQTEKKDLMSVIFNFIRNLLSETIFKSSRNCESNVHEQNTQEEEIH
PCERPKTPCERPITPPAPKFCEDEEATGGALSGLTKMVANQLDNCMNGQMVEHLMDSVMKLCLIIAKSCD
SPLSELGEEKCGDASRPNSAFPDNLYECLPVKGTGTAEALLQNAYLTIHNELRGLSGQPPEGCEIPKVIV
SNHNLADTVQNKQLQAVLQWVAASELNVPILYFAGDDEGIQEKLLQLSATAVEKGRSVGEVLQSVLRYEK
ERQLDEAVGNVTRLQLLDWLMANL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009650
ORF Size 2592 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009650.2, NP_033780.2
RefSeq Size 3173 bp
RefSeq ORF 2595 bp
Locus ID 11642
UniProt ID O88987
Cytogenetics 6 F3
Summary May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009650" in other vectors (4)
SKU Description Size Price
MC222248 Akap3 (untagged) - Mouse A kinase (PRKA) anchor protein 3 (Akap3), (10ug) 10 ug
$871.00
MR215888 Akap3 (Myc-DDK-tagged) - Mouse A kinase (PRKA) anchor protein 3 (Akap3) 10 ug
$589.00 $791.00
MR215888L3 Lenti ORF clone of Akap3 (Myc-DDK-tagged) - Mouse A kinase (PRKA) anchor protein 3 (Akap3) 10 ug
$1,091.00
MR215888L4 Lenti ORF clone of Akap3 (mGFP-tagged) - Mouse A kinase (PRKA) anchor protein 3 (Akap3) 10 ug
$1,091.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.