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Pgam5 (NM_001163538) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215881
Pgam5 (tGFP-tagged) - Mouse phosphoglycerate mutase family member 5 (Pgam5) nuclear gene encoding mitochondrial protein transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pgam5
Synonyms 2610528A17Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215881 representing NM_001163538
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTTCCGGCAGGCGCTTCAGCTGGCGGCCTGTGGTCTGGCCGGTGGCTCGGCCGCTGTTCTCTTCT
CGGCGGTCGCGGTAGGGAAGCCGCGAGGTGGCGGGGATGCAGACACGCGCGCGACCGAGCCGCCGGCCTG
GACAGGAGCGCGAGCCGGGCGCGGCGTCTGGGACACCAACTGGGACAGGCGAGAGCCACTGTCACTCATT
AACCTGAAGAAAAGGAATGTGGAATCTGGAGAAGACGAGTTGACATCCAGGCTGGATCACTATAAGGCCA
AGGCCACAAGGCACATCTTCCTCATCCGGCATTCCCAGTACCATGTGGATGGCTCCCTGGAGAAGGACCG
CACCCTGACACCATTAGGTCGGGAACAGGCTGAACTCACGGGGCTCCGACTTGCAAGCCTGGGATTAAAG
TTTAATAAAATTGTCCATTCCTCTATGACCCGTGCAGTAGAGACCACAGACATCATCAGCAAGCACCTGC
CAGGTGTCTCCAGAGTCAGCACAGACTTGCTACGGGAAGGTGCCCCCATTGAACCGGATCCACCTGTCTC
TCACTGGAAGCCAGAGGCTGTGTATTATGAAGATGGAGCCCGGATTGAAGCTGCCTTCAGGAACTACATC
CACCGAGCTGATGCCAGGCAGGAGGAGGACAGCTATGAGATCTTCATATGCCATGCCAATGTCATCCGCT
ATATTGTTTGTAGAGCGTTGCAGTTTCCCCCAGAAGGTTGGCTCCGCCTGTCCCTCAACAACGGCAGTAT
CACCCACCTGGTGATTCGACCCAATGGTCGTGTGGCACTCAGGACCCTTGGGGACACAGGGTTCATGCCC
CCAGACAAGATTACTCGGTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215881 representing NM_001163538
Red=Cloning site Green=Tags(s)

MAFRQALQLAACGLAGGSAAVLFSAVAVGKPRGGGDADTRATEPPAWTGARAGRGVWDTNWDRREPLSLI
NLKKRNVESGEDELTSRLDHYKAKATRHIFLIRHSQYHVDGSLEKDRTLTPLGREQAELTGLRLASLGLK
FNKIVHSSMTRAVETTDIISKHLPGVSRVSTDLLREGAPIEPDPPVSHWKPEAVYYEDGARIEAAFRNYI
HRADARQEEDSYEIFICHANVIRYIVCRALQFPPEGWLRLSLNNGSITHLVIRPNGRVALRTLGDTGFMP
PDKITRS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001163538
ORF Size 861 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163538.1, NP_001157010.1
RefSeq Size 2079 bp
RefSeq ORF 867 bp
Locus ID 72542
UniProt ID Q8BX10
Cytogenetics 5 F
Summary Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression (By similarity). Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001163538" in other vectors (4)
SKU Description Size Price
MC211339 Pgam5 (untagged) - Mouse phosphoglycerate mutase family member 5 (Pgam5), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug) 10 ug
$300.00
MR215881 Pgam5 (Myc-DDK-tagged) - Mouse phosphoglycerate mutase family member 5 (Pgam5), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$289.00 $300.00
MR215881L3 Lenti ORF clone of Pgam5 (Myc-DDK-tagged) - Mouse phosphoglycerate mutase family member 5 (Pgam5), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$600.00
MR215881L4 Lenti ORF clone of Pgam5 (mGFP-tagged) - Mouse phosphoglycerate mutase family member 5 (Pgam5), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$600.00

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