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Cse1l (NM_023565) Mouse Tagged ORF Clone

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SKU
MG215858
Cse1l (tGFP-tagged) - Mouse chromosome segregation 1-like (S. cerevisiae) (Cse1l), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,089.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cse1l
Synonyms 2610100P18Rik; AA407533; Capts; Cas; Xpo2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215858 representing NM_023565
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTCAGCGATGCGAATTTACAGACACTAACAGAGTATTTAAAGAAAACCCTGGATCCTGATCCTG
CCATTAGACGGCCAGCTGAGAAGTTTCTCGAATCCGTAGAAGGAAATCAGAATTACCCACTGTTGCTTTT
AACATTATTGGAAAAGTCACAGGATAATGTCATTAAAGTATGTGCTTCAGTAACCTTCAAGAACTATATT
AAAAGGAATTGGAGAATTGTTGAAGATGAACCAAACAAAATTTGCGAAGCTGATCGAGTAGCTATTAAAG
CCAACATAGTGCATCTGATGCTGAGCAGTCCAGAGCAGATTCAGAAGCAGCTGAGTGATGCTATTAGCAT
CATTGGCAGAGAAGATTTCCCTCAGAAATGGCCTGACTTGTTGACAGAAATGGTGAATCGCTTTCAGAGT
GGAGATTTTCACGTCATTAATGGAGTCCTTCGTACAGCGCACTCACTATTTAAGAGATACCGGCATGAGT
TCAAGTCAAATGAGCTGTGGACTGAAATTAAACTTGTCTTGGATGCCTTTGCTTTGCCTTTGACTAATCT
ATTCAAGGCCACCATTGAACTGTGCAGTACCCATGCAAATGACGCCTCTGCCCTGAGGATTCTTTTTTCT
TCCTTGATTTTGATCTCAAAATTGTTCTATAGTTTGAACTTTCAGGATCTCCCTGAATTCTTTGAAGATA
ATATGGAAACTTGGATGAACAATTTTCATACCCTCCTGACACTGGATAATAAGCTCTTGCAGACTGATGA
TGAAGAGGAAGCAGGCTTACTGGAGCTTTTAAAGTCTCAGATCTGTGATAATGCCGCTCTCTATGCACAG
AAATATGACGAAGAGTTTCAGCGGTACCTCCCTCGCTTTGTCACAGCTATCTGGAACTTACTCGTTACAA
CAGGGCGAGAAGTTAAATATGACTTGTTGGTTAGCAATGCAATTCAGTTTCTGGCTTCAGTTTGTGAAAG
GCCACATTATAAGAATCTGTTTGAGGACCAGAACACGCTGACAAGTATCTGTGAAAAAGTTATTGTGCCT
AACATGGAATTTAGAGCTGCTGATGAAGAAGCTTTTGAAGATAATTCTGAGGAGTACATACGGAGGGATT
TGGAAGGCTCTGATATTGATACTAGACGCAGGGCTGCTTGTGATCTTGTACGAGGATTATGCAAATTTTT
TGAAGGTCCTGTGACAGGAATCTTCTCTGGTTATGTTAATTCCATGCTCCAGGAGTATGCAAAAAACCCG
TCTGTCAACTGGAAACACAAAGATGCAGCTATTTACCTTGTCACATCTTTGGCATCAAAAGCCCAGACAC
AGAAGCATGGAATCACGCAAGCCAATGAACTTGTAAACCTAACTGAATTTTTCGTGAATCACATACTTCC
TGATTTAAAATCAAATAATGTGAATGAGTTTCCTGTGCTTAAAGCTGATGGTATCAAATATATTATGATT
TTTAGGAATCAAGTACCAAAAGAGCACCTCCTAGTCTCTATTCCCCTCTTGATCAGTCACCTGGAGGCTG
AGAGTATTGTCGTCCACACTTATGCCGCACATGCTCTCGAAAGGCTGTTCACTATGAGAGGGTCAAACAA
TACCACTCTTTTTACAGCTGCAGAAATCGCACCGTTTGTTGAGATTCTGCTAACAAACCTTTTCAAAGCT
CTCACACTTCCTGGCTCTTCAGAAAATGAATATATTATGAAAGCTATCATGAGAAGTTTCTCCCTCCTGC
AAGAGGCCATCATCCCCTATATCCCCACTCTCATCACGCAGCTCACACAGAAGCTGCTGGCTGTTAGTAA
GAATCCCAGTAAACCTCATTTCAATCACTACATGTTTGAAGCAATATGCTTGTCTATAAGAATAACCTGC
AAAGCTAACCCTGCTGCTGTTGTGAATTTTGAGGAGGCTCTGTTTCTAGTGTTCACTGAGATCTTACAAA
ATGATGTACAAGAGTTTATCCCATATGTCTTTCAAGTGATGTCTTTACTTCTGGAAACACATAAAAATGA
TATCCCGTCTTCCTATATGGCCTTGTTTCCCCACCTCCTTCAGCCAGTACTGTGGGAAAGGACAGGAAAC
ATCCCTGCTCTAGTGAGGCTGCTCCAGGCATTCTTAGAACGAGGCTCAAGTACAATAGCAACTGCTGCAG
CTGACAAAATTCCTGGATTACTGGGTGTCTTCCAGAAGCTGATTGCATCCAAGGCAAACGATCACCAAGG
TTTCTATCTTCTCAACAGTATAATCGAACACATGCCTCCTGAATCAGTTGACCAGTATAGGAAGCAGATC
TTCATTCTACTATTCCAGAGACTGCAAAACTCCAAGACCACCAAGTTTATCAAGAGTTTCTTAGTGTTTA
TTAACTTATATTGCATTAAGTATGGGGCACTAGCATTACAAGAAATATTTGATGGCATACAACCAAAAAT
GTTTGGAATGGTTTTGGAAAAAATCATTATTCCAGAGATTCAAAAGGTATCTGGGAACGTGGAGAAAAAA
ATCTGTGCAGTTGGCATAACCAAATTACTCACAGAGTGTCCACCAATGATGGACACGGAGTACACTAAGC
TGTGGACTCCTTTGTTACAATCTCTCATTGGCCTGTTTGAATTACCTGAAGATGACAGCATTCCTGATGA
AGAGCATTTCATTGACATAGAAGATACACCAGGATACCAGACTGCCTTCTCACAGCTGGCATTTGCTGGT
AAAAAGGAACATGATCCTGTAGGTCAGATGGTAAACAACCCCAAAATCCATCTGGCACAGTCACTTCACA
AACTGTCAACTGCTTGTCCTGGAAGGGTTCCCTCGATGGTGAGCACCAGCCTCAATGCAGAAGCCTTGCA
GTATCTCCAAGGTTACCTGCAGGCAGCCAGTGTGACCCTGTTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215858 representing NM_023565
Red=Cloning site Green=Tags(s)

MELSDANLQTLTEYLKKTLDPDPAIRRPAEKFLESVEGNQNYPLLLLTLLEKSQDNVIKVCASVTFKNYI
KRNWRIVEDEPNKICEADRVAIKANIVHLMLSSPEQIQKQLSDAISIIGREDFPQKWPDLLTEMVNRFQS
GDFHVINGVLRTAHSLFKRYRHEFKSNELWTEIKLVLDAFALPLTNLFKATIELCSTHANDASALRILFS
SLILISKLFYSLNFQDLPEFFEDNMETWMNNFHTLLTLDNKLLQTDDEEEAGLLELLKSQICDNAALYAQ
KYDEEFQRYLPRFVTAIWNLLVTTGREVKYDLLVSNAIQFLASVCERPHYKNLFEDQNTLTSICEKVIVP
NMEFRAADEEAFEDNSEEYIRRDLEGSDIDTRRRAACDLVRGLCKFFEGPVTGIFSGYVNSMLQEYAKNP
SVNWKHKDAAIYLVTSLASKAQTQKHGITQANELVNLTEFFVNHILPDLKSNNVNEFPVLKADGIKYIMI
FRNQVPKEHLLVSIPLLISHLEAESIVVHTYAAHALERLFTMRGSNNTTLFTAAEIAPFVEILLTNLFKA
LTLPGSSENEYIMKAIMRSFSLLQEAIIPYIPTLITQLTQKLLAVSKNPSKPHFNHYMFEAICLSIRITC
KANPAAVVNFEEALFLVFTEILQNDVQEFIPYVFQVMSLLLETHKNDIPSSYMALFPHLLQPVLWERTGN
IPALVRLLQAFLERGSSTIATAAADKIPGLLGVFQKLIASKANDHQGFYLLNSIIEHMPPESVDQYRKQI
FILLFQRLQNSKTTKFIKSFLVFINLYCIKYGALALQEIFDGIQPKMFGMVLEKIIIPEIQKVSGNVEKK
ICAVGITKLLTECPPMMDTEYTKLWTPLLQSLIGLFELPEDDSIPDEEHFIDIEDTPGYQTAFSQLAFAG
KKEHDPVGQMVNNPKIHLAQSLHKLSTACPGRVPSMVSTSLNAEALQYLQGYLQAASVTLL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023565
ORF Size 2913 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023565.3, NP_076054.1
RefSeq Size 3604 bp
RefSeq ORF 2916 bp
Locus ID 110750
UniProt ID Q9ERK4
Cytogenetics 2 87.22 cM
Summary Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_023565" in other vectors (4)
SKU Description Size Price
MC222918 Cse1l (untagged) - Mouse chromosome segregation 1-like (S. cerevisiae) (Cse1l), (10ug) 10 ug
$979.00
MR215858 Cse1l (Myc-DDK-tagged) - Mouse chromosome segregation 1-like (S. cerevisiae) (Cse1l) 10 ug
$589.00 $889.00
MR215858L3 Lenti ORF clone of Cse1l (Myc-DDK-tagged) - Mouse chromosome segregation 1-like (S. cerevisiae) (Cse1l) 10 ug
$1,189.00
MR215858L4 Lenti ORF clone of Cse1l (mGFP-tagged) - Mouse chromosome segregation 1-like (S. cerevisiae) (Cse1l) 10 ug
$1,189.00

file-alt Citations

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