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Klhdc3 (NM_027910) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215851
Klhdc3 (tGFP-tagged) - Mouse kelch domain containing 3 (Klhdc3) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Klhdc3
Synonyms 1300011D16Rik; Peas
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215851 representing NM_027910
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTACGGTGGACAGTGCATCTGGAGGGCGGGCCACGCAGGGTGAACCATGCTGCAGTGGCTGTCGGGC
ACCGAGTATATTCCTTCGGGGGTTACTGCTCTGGGGAAGACTACGAAACACTACGGCAGATAGATGTGCA
CATTTTCAATGCTGTGTCCTTGCGTTGGACAAAGCTGCCCCCAGTGAGGCCTGCCGTCCGAGGGCAGGCT
CCTGTCGTTCCCTACATGCGCTATGGGCACTCAACCGTCCTCATTGATGACACCGTCTTCCTTTGGGGTG
GGCGCAATGACACTGAAGGGGCCTGCAACGTACTCTACGCCTTTGATGTCAATACTCACAAGTGGTCTAC
ACCCCGAGTGTCAGGGACAGTTCCTGGGGCACGGGACGGACATTCAGCTTGTGTCCTGGGCAAGATCATG
TACATTTTTGGGGGATATGAACAGCTGGCCGACTGCTTTTCCAACGACATCCACAAGCTGGATACCAGCA
CCATGACATGGACTCTTGTTTGTACAAAGGGCAATCCTGCACGTTGGAGGGACTTCCACTCAGCCACAAT
GCTGGGCAATCACATGTATGTCTTTGGGGGCCGTGCAGACCGCTTTGGGCCATTTCATTCCAACAATGAG
ATCTACTGTAATCGAATTCGAGTCTTTGATACCAGAACGGAGGCCTGGCTCGACTGTCCTCACACGCCGG
TGCTGCCTGAGGGGCGCAGGAGCCATTCAGCCTTTGGCTACAATGGAGAGCTGTACATCTTTGGTGGCTA
CAATGCAAGGCTGAATCGGCATTTCCATGACCTCTGGAAGTTTAATCCTGGGTCCTTTACCTGGAAGAAG
ATTGAACCAAAGGGGAAAGGACCGTGTCCCCGCCGCCGCCAGTGTTGCTGTATTGTTGGTGATAAAATTG
TCCTCTTTGGCGGTACCAGCCCCTCTCCCGAGGAAGGCCTGGGGGATGAATTTGACCTCATAGATCATTC
TGACTTACACATCTTGGACTTCAGCCCTAGTCTGAAGACTCTGTGCAAGCTGGCGGTGATTCAGTATAGC
CTGGACCAGTCCTGTCTGCCCCATGACATCAGGTGGGAGCTCAACGCCATGACCACCAACAGCAATATCA
GCCGCCCCATTGTCTCCTCCCATGGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215851 representing NM_027910
Red=Cloning site Green=Tags(s)

MLRWTVHLEGGPRRVNHAAVAVGHRVYSFGGYCSGEDYETLRQIDVHIFNAVSLRWTKLPPVRPAVRGQA
PVVPYMRYGHSTVLIDDTVFLWGGRNDTEGACNVLYAFDVNTHKWSTPRVSGTVPGARDGHSACVLGKIM
YIFGGYEQLADCFSNDIHKLDTSTMTWTLVCTKGNPARWRDFHSATMLGNHMYVFGGRADRFGPFHSNNE
IYCNRIRVFDTRTEAWLDCPHTPVLPEGRRSHSAFGYNGELYIFGGYNARLNRHFHDLWKFNPGSFTWKK
IEPKGKGPCPRRRQCCCIVGDKIVLFGGTSPSPEEGLGDEFDLIDHSDLHILDFSPSLKTLCKLAVIQYS
LDQSCLPHDIRWELNAMTTNSNISRPIVSSHG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027910
ORF Size 1146 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027910.3, NP_082186.2
RefSeq Size 2139 bp
RefSeq ORF 1149 bp
Locus ID 71765
UniProt ID Q8VEM9
Cytogenetics 17 C
Summary May be involved in meiotic recombination process.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027910" in other vectors (5)
SKU Description Size Price
MC204532 Klhdc3 (untagged) - Mouse kelch domain containing 3 (Klhdc3), transcript variant 1, (10ug) 10 ug
$457.00
MG205971 Klhdc3 (tGFP-tagged) - Mouse kelch domain containing 3 (Klhdc3) 10 ug
$657.00
MR215851 Klhdc3 (Myc-DDK-tagged) - Mouse kelch domain containing 3 (Klhdc3), transcript variant 1 10 ug
$289.00 $457.00
MR215851L3 Lenti ORF clone of Klhdc3 (Myc-DDK-tagged) - Mouse kelch domain containing 3 (Klhdc3), transcript variant 1 10 ug
$757.00
MR215851L4 Lenti ORF clone of Klhdc3 (mGFP-tagged) - Mouse kelch domain containing 3 (Klhdc3), transcript variant 1 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.