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Kazn (NM_144531) Mouse Tagged ORF Clone

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SKU
MG215840
Kazn (tGFP-tagged) - Mouse RIKEN cDNA 9030409G11 gene (9030409G11Rik) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $913.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kazn
Synonyms 2310007B04Rik; 9030409G11Rik; AI256607; AI666764; AW061099; Kaz; Kazrin
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215840 representing NM_144531
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGAAGACAATAAGCAGCTCGCGCTCCGCATCGATGGGGCGGTCCAGTCGGCCAGCCAGGAGGTGA
CCAACCTGCGAGCCGAACTCACGGCCACCAACCGGAGACTGGCGGAATTGAGCGGCGGAGGCGGCGGCCC
CGGCTCGGGCCCGGGAGCTGCGACCAGCGCCTCGGCAGCAGCGGTGACGGTGGCTGACTCGGCAGTGGCG
ACCATGGAGAACCATCAGCACGGAGCTCAAGTGCTCCTGCGGGAGGAAGTGGTCCAGCTTCAAGAGGAAG
TGCACCTTCTCCGGCAGATGAAGGAAATGCTGGCGAAGGACCTGGAGGAGTCACAGGGCGGCAAGTGCTC
TGAGGTCCTCTCAGCCACTGAGCTCCGGGTGCAGCTGGTACAGAAGGAGCAGGAACTGGCCAGAGCCAAA
GAAGCCTTGCAAGCCATGAAAGCTGACCGGAAGCGCTTAAAGGGCGAGAAGACTGACCTGGTGAGCCAGA
TGCAGCAGCTGTACGCCACGCTGGAGAGCCGCGAGGAGCAGTTGCGAGACTTCATCCGCAACTATGAGCA
GCACCGCAAGGAGAGTGAAGACGCAGTCAAAGCACTGGCCAAGGAGAAGGACCTGCTGGAGCGAGAGAAG
TGGGAGCTTCGACGGCAGGCCAAGGAAGCCACAGACCATGCTGCGGCCCTGCGCTCCCAGCTGGACCTCA
AGGACAACCGCATGAAGGAGCTGGAGGCGGAGCTCGCCATGGCCAAGCAGTCCTTAGCCACTCTGACCAA
GGATGTCCCCAAGAGGCATTCCCTCGCCATGCCCGGTGAGACGGTGCTCAATGGCAACCAGGAATGGGTG
GTGCAGGCTGACCTCCCACTGACTGCTGCCATCCGGCAAAGCCAACAGACTCTCTACCACTCACACCCTC
CCCACCCTGCAGACCGGCAAGTCAGGGTGAGCCCCTGCCACTCAAGACAGCCCTCCGTCATCTCTGATGC
TTCTGCTGCTGAAGGTGACCGGTCATCTACACCAAGCGACATCAACTCCCCAAGACACCGGACACACTCC
CTCTGCAACGGCGACAGTCCCGGCCCAGTTCAGAAGAGCCTACACAACCCTATTGTACAGTCACTAGAGG
ATCTTGAAGACCAAAAACGGAAAAAGAAGAAGGAGAAGATGGGATTCGGCTCCATCTCTCGAGTCTTCGC
CAGAGGGAAGCAACGGAAGTCCCTCGACCCGGGCCTCTTTGATGACTCCGACAGCCAGTGCAGCCCCACA
CGCCACAGCCTCAGCCTGTCTGAGGGAGAGGAGCAGATGGACCGTCTGCAGCACGTGGAGCTGGTGAGGA
CCACGCCCATGTCGCACTGGAAGGCTGGCACAGTGCAGGCCTGGCTGGAGGTGGTGATGGCCATGCCCAT
GTACGTCAAGGCCTGTGCAGAAAATGTCAAGAGTGGGAAGGTGCTTCTCAGCCTGAGTGACGAGGACCTG
GAGTTGGGCCTGGGAGTGTGCAGCTCCTTGCACAGGCGCAAGCTCCGCCTGGCCATTGAGGACTACCGAG
ATGCTGAGGCAGGCCGCAGCCTGTCCAAAGCTGCCGACCTGGACCATCACTGGGTGGCCAAGGCCTGGCT
GAATGACATTGGCCTGTCCCAGTATTCCCAGGCCTTCCAGAACCACCTGGTTGATGGGAGGATGCTGAAT
TCACTGATGAAGCGGGACCTGGAGAAACACCTGAACGTGTCCAAGAAGTTCCACCAAGTTAGCATCTTGC
TGGGAATTGAGCTGCTGTACCAAGTGAACTTCAGCAGGGAGGCTCTCCAGGAGCGCAGAGCCCGCTGTGA
AACACAGAACACAGACCCTGTGGTCTGGACCAACCAGCGGGTGCTCAAGTGGGTGCGCGATATCGACCTG
AAGGAGTATGCAGACAACCTGACCAATAGCGGTGTCCATGGTGCTGTGCTGGTCCTGGAGCCCACATTCA
ATGCCGAGGCCATGGCCACCGCCCTGGGCATCCCCAGCGGGAAGCACATACTCAGGAGACACCTGGCAGA
GGAGATGAGCACCATCTTCCATCCATCCAACTCCACAGGCATCCGGGAGTCTGAGCGCTTTGGGACACCA
CCTGGCAGGGCCTCTAGCATCACCAGAGCTGGGAGAGAGGACAGTGGGGGCAATAGCAAGCACAGAGCTG
GCCGGCTGCCCCTGGGGAAGATAGGAAGGGGCTTCAGCAGCAAAGAGCCCGATTTCCATGATGACTATGG
CTCTCTGGAGAACGAGGACTGTGGGGATGAGGACCTCCAAGGCAGGCCGGAACAGTGCCGTCTGGAGGGA
TACGGCAGCTTGGAGGTCACCAATGTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215840 representing NM_144531
Red=Cloning site Green=Tags(s)

MMEDNKQLALRIDGAVQSASQEVTNLRAELTATNRRLAELSGGGGGPGSGPGAATSASAAAVTVADSAVA
TMENHQHGAQVLLREEVVQLQEEVHLLRQMKEMLAKDLEESQGGKCSEVLSATELRVQLVQKEQELARAK
EALQAMKADRKRLKGEKTDLVSQMQQLYATLESREEQLRDFIRNYEQHRKESEDAVKALAKEKDLLEREK
WELRRQAKEATDHAAALRSQLDLKDNRMKELEAELAMAKQSLATLTKDVPKRHSLAMPGETVLNGNQEWV
VQADLPLTAAIRQSQQTLYHSHPPHPADRQVRVSPCHSRQPSVISDASAAEGDRSSTPSDINSPRHRTHS
LCNGDSPGPVQKSLHNPIVQSLEDLEDQKRKKKKEKMGFGSISRVFARGKQRKSLDPGLFDDSDSQCSPT
RHSLSLSEGEEQMDRLQHVELVRTTPMSHWKAGTVQAWLEVVMAMPMYVKACAENVKSGKVLLSLSDEDL
ELGLGVCSSLHRRKLRLAIEDYRDAEAGRSLSKAADLDHHWVAKAWLNDIGLSQYSQAFQNHLVDGRMLN
SLMKRDLEKHLNVSKKFHQVSILLGIELLYQVNFSREALQERRARCETQNTDPVVWTNQRVLKWVRDIDL
KEYADNLTNSGVHGAVLVLEPTFNAEAMATALGIPSGKHILRRHLAEEMSTIFHPSNSTGIRESERFGTP
PGRASSITRAGREDSGGNSKHRAGRLPLGKIGRGFSSKEPDFHDDYGSLENEDCGDEDLQGRPEQCRLEG
YGSLEVTNV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144531
ORF Size 2337 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144531.3, NP_653114.3
RefSeq Size 4914 bp
RefSeq ORF 2340 bp
Locus ID 71529
UniProt ID Q69ZS8
Cytogenetics 4 E1
Summary Component of the cornified envelope of keratinocytes. May be involved in the interplay between adherens junctions and desmosomes. The function in the nucleus is not known.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_144531" in other vectors (4)
SKU Description Size Price
MC221577 Kazn (untagged) - Mouse RIKEN cDNA 9030409G11 gene (9030409G11Rik), transcript variant 1, (10ug) 10 ug
$786.00
MR215840 Kazn (Myc-DDK-tagged) - Mouse RIKEN cDNA 9030409G11 gene (9030409G11Rik), transcript variant 1 10 ug
$589.00 $713.00
MR215840L3 Lenti ORF clone of Kazn (Myc-DDK-tagged) - Mouse RIKEN cDNA 9030409G11 gene (9030409G11Rik), transcript variant 1 10 ug
$1,013.00
MR215840L4 Lenti ORF clone of Kazn (mGFP-tagged) - Mouse RIKEN cDNA 9030409G11 gene (9030409G11Rik), transcript variant 1 10 ug
$1,013.00

file-alt Citations

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