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Slc22a16 (NM_027572) Mouse Tagged ORF Clone

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SKU
MG215814
Slc22a16 (tGFP-tagged) - Mouse solute carrier family 22 (organic cation transporter) member 16 (Slc22a16), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $804.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc22a16
Synonyms 4921504E14Rik; CT2; FLIPT2; OCT6; OKB1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215814 representing NM_027572
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCCTGCAACGTGGAGCTGATTTTTGACCACATAGGACACTTCGGCAGGTTCCAGATAGTCCTGT
ATCTGATATGTGCCTACCAGAGCCTCTCTTGTGGTATCCACTATCTGTCTTCTGTGTTCCTGTCAATTAT
CCCTGAGCATGCGTGCAAGCCCCCAGGCATGGTGCGGAAGGCTGTTTTCCACAACGTATCTGCTTGGAGG
TTGGAGGACATATTGGCTCTGCGGTCACCAGAGCACAAAGATCACATCATGGTGGAGCTGCAGGATGGGG
AGATCTGGGAACTCACGAGATGTAGCAGGACTTGGAGGGAGAACACGTCACATTTGGGCTATGAGTACAG
TGGTTATAAGCATGACAGTCCTTGCTTCGATGGCTACGTCTATGATCAGAGCAAATGGAGGAATTCTGCG
GTGAGAAATTTCAACCTGGTCTGTGATCAGAAATGGTATGCAAGAATGATCCAGCCCTTGATTATATTTG
GCGTGATGCTGGGATCAATTACTTTTAGCTACCTTTCTGACAGATTTGGAAGGCGAATGGCCCTGTGGTG
CACGAGCATCGGCGTGTTCTTCTTTGGAATAGCATCCCTCTTTATATTTGATTATCTCAGCTTCATGATC
ACACGCTTTTTCCTTGTGATGGCCTCGAGTGGCTATTTTGTGGTGGTCTTTGTCTATGTAATGGAGATCA
TTGGCAAAAAGGCTCGCACATGGGCATCCATACATCTCAATACCTTCTTTGCTATTGGAGCAATGTTGGT
GGCTTTGGCAAGCTATTTGTTGAAAACGTGGTGGCTCTATCAGATCATCCTGTGCATCGTGACTACCCCC
TTCATCCTGTGCTGTTGGATGCTTCCAGAGACTCCCTTTTGGCTCCTCTCAGAAGGAAGATACAAGGAAG
CACAAGGCACCGTGGACACCATGGCTGTGTGGAATAAGTCCAGCTCCTGTGACCTGGTAGAGCTCTTATC
ACTAGATGTGACGAGGTCCCACAATAAAAGCCCCCACAGCATTAGGAAGCACAGGCTAGCAGATCTGTTT
CATAACCTGGATGTCGCAAAGATGACCCTTATCGTTTGGCTGGATTGGTTCACGGCGAATTTGGGATACT
ACATGTTTGGCAAGGAGGTTATTCGGAGAAAAGAAAATGAACCCCTTTACCTCCTCCTAGTGGGTGCTAT
GGAAATCCCTGCCTACATCTGTCTGTGCATCTGGTTGAAGAGGGTGGGAAGAAGAAAGACTATGCTCCTC
TTCCTCTTGGTGTCCTCGCTCACCTGTATGCTGCATGTGGTGATGCCCTCGGATTACAAGACTGCGAAAA
GGATGGTAGCTTTACTTGTCAAGAGTGTTATAAGTTCCGTATTTGCCTTTATTTATCTTTATACGGCGGA
GCTGTATCCAACAACCGTAAGGTGCTTGGCTGTAGGGAGCAGCAACATGGTGTCCCACGTGTCGAGCATC
TTCATCCCATTCACCAGTCACTTCTCCAAAGTCTGGATCTTCCTGCCGCAGATTTTATTTGGGATCCTGG
CCATACTGAGTGGATTGTTGTCATTGAAGCTTCCAGAAACCCAGGACACACCAATGAAATCTACTTGGGA
GACGACTGAACAGCAGGTGCCAGAAAACAAGGATAGTTTAGGCGAAGGCCCTCCTGATAGTTTTGAGAGA
TGGGATTCAAGCAGAGCCCTTTCTTTTGCTGAGAGGTGGGGTTTAAGCAGAGCCTCTCCTGATGCTGAGA
AGTGGGGTTCAGGCAGAGTCCCTCCTGATGCTGGGAAGTGGGGTGCAGGCATAGCCCCTCCTGTGACTGA
GAGGGGTGCTTCAGGCAGAGCTTCTCTTGAAGATGAGAGTGGGGGTTCAGGCAGAGCCCCTCCTGAAAAG
AACACTGAGATGGAAAATGAGATAGAAAACATGAAAGTGAGTAACCTGGGGGGATTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215814 representing NM_027572
Red=Cloning site Green=Tags(s)

MESCNVELIFDHIGHFGRFQIVLYLICAYQSLSCGIHYLSSVFLSIIPEHACKPPGMVRKAVFHNVSAWR
LEDILALRSPEHKDHIMVELQDGEIWELTRCSRTWRENTSHLGYEYSGYKHDSPCFDGYVYDQSKWRNSA
VRNFNLVCDQKWYARMIQPLIIFGVMLGSITFSYLSDRFGRRMALWCTSIGVFFFGIASLFIFDYLSFMI
TRFFLVMASSGYFVVVFVYVMEIIGKKARTWASIHLNTFFAIGAMLVALASYLLKTWWLYQIILCIVTTP
FILCCWMLPETPFWLLSEGRYKEAQGTVDTMAVWNKSSSCDLVELLSLDVTRSHNKSPHSIRKHRLADLF
HNLDVAKMTLIVWLDWFTANLGYYMFGKEVIRRKENEPLYLLLVGAMEIPAYICLCIWLKRVGRRKTMLL
FLLVSSLTCMLHVVMPSDYKTAKRMVALLVKSVISSVFAFIYLYTAELYPTTVRCLAVGSSNMVSHVSSI
FIPFTSHFSKVWIFLPQILFGILAILSGLLSLKLPETQDTPMKSTWETTEQQVPENKDSLGEGPPDSFER
WDSSRALSFAERWGLSRASPDAEKWGSGRVPPDAGKWGAGIAPPVTERGASGRASLEDESGGSGRAPPEK
NTEMENEIENMKVSNLGGF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027572
ORF Size 1947 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027572.1, NP_081848.1
RefSeq Size 2377 bp
RefSeq ORF 1950 bp
Locus ID 70840
UniProt ID Q497L8
Cytogenetics 10 B1
Summary High affinity carnitine transporter; the uptake is partially sodium-ion dependent. Thought to mediate the L-carnitine secretion mechanism from testis epididymal epithelium into the lumen which is involved in the maturation of spermatozoa. Also transports organic cations such as tetraethylammonium (TEA) and doxorubicin. The uptake of TEA is inhibited by various organic cations. The uptake of doxorubicin is sodium-independent (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027572" in other vectors (4)
SKU Description Size Price
MC220096 Slc22a16 (untagged) - Mouse solute carrier family 22 (organic cation transporter), member 16 (Slc22a16), (10ug) 10 ug
$665.00
MR215814 Slc22a16 (Myc-DDK-tagged) - Mouse solute carrier family 22 (organic cation transporter), member 16 (Slc22a16) 10 ug
$289.00 $604.00
MR215814L3 Lenti ORF clone of Slc22a16 (Myc-DDK-tagged) - Mouse solute carrier family 22 (organic cation transporter), member 16 (Slc22a16) 10 ug
$904.00
MR215814L4 Lenti ORF clone of Slc22a16 (mGFP-tagged) - Mouse solute carrier family 22 (organic cation transporter), member 16 (Slc22a16) 10 ug
$904.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.