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Ndufa12 (NM_025551) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215813
Ndufa12 (tGFP-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 12 (Ndufa12) nuclear gene encoding mitochondrial protein, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ndufa12
Synonyms 2410011G03Rik; AW112974
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215813 representing NM_025551
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGATGAGAATGGAGCTGGTGGAGGTCCTGAAGCGCGGCGTGCAGCAGGTCACCGGCCACGGCGGCC
TGCGGGGCCTCCTACGGGTTTTCTTCAGGGCAAATGATATAAGGATTGGTACACTGGTGGGAGAAGACAA
ATATGGAAATAAATACTACGAAGACAACAAGCAATTTTTTGGCCGCCACCGATGGGTCATCTACACCACC
GAGATGAACGGCAAAAACACATTCTGGGATGTGGATGGAAGCATGGTGCCTCCTGAGTGGCACCGCTGGC
TTCACTGCATGACTGACGACCCTCCGACGACTAATCCACCGACTGCTCGTAAATTCATCTGGACAAACCA
TAAATTCAATGTGTCTGCCACTCCAGAGCAATACGTTCCTTATTCCACCACTAGAAAGGAGATTCATGAA
TGGGTTCCACCTTCGACACCTTACAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215813 representing NM_025551
Red=Cloning site Green=Tags(s)

MQMRMELVEVLKRGVQQVTGHGGLRGLLRVFFRANDIRIGTLVGEDKYGNKYYEDNKQFFGRHRWVIYTT
EMNGKNTFWDVDGSMVPPEWHRWLHCMTDDPPTTNPPTARKFIWTNHKFNVSATPEQYVPYSTTRKEIHE
WVPPSTPYK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025551
ORF Size 447 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025551.3, NP_079827.2
RefSeq Size 561 bp
RefSeq ORF 450 bp
Locus ID 66414
UniProt ID Q7TMF3
Cytogenetics 10 C2
Summary Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025551" in other vectors (4)
SKU Description Size Price
MC210362 Ndufa12 (untagged) - Mouse NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12 (Ndufa12), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$165.00
MR215813 Ndufa12 (Myc-DDK-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12 (Ndufa12), nuclear gene encoding mitochondrial protein 10 ug
$289.00
MR215813L3 Lenti ORF clone of Ndufa12 (Myc-DDK-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12 (Ndufa12), nuclear gene encoding mitochondrial protein 10 ug
$450.00
MR215813L4 Lenti ORF clone of Ndufa12 (mGFP-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12 (Ndufa12), nuclear gene encoding mitochondrial protein 10 ug
$450.00

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