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Arhgap21 (NM_001128084) Mouse Tagged ORF Clone

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SKU
MG215805
Arhgap21 (tGFP-tagged) - Mouse Rho GTPase activating protein 21 (Arhgap21) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,843.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Arhgap21 or NM_001128084"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Arhgap21
Synonyms 5530401C11Rik; AA416458; ARHGAP10
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215805 representing NM_001128084
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACACATTGGACTGGTCTGCCTGAGGAAGATGGTGACAAACTCAAGGCCTGTGGGGCGGCCTCTG
CCTGTGAGGTGTCAAAAAACAAAGATGGAAAAGACCAAGGTGAACCTGTGTCACCGTCTGAAGATGAGCC
ATTCTCCTGGCCAGGTCCCAAAACAGTCATGCTGAAGAGAACATCTCAAGGGTTTGGTTTCACATTACGA
CACTTCATCGTTTATCCCCCAGAGTCTGCAATCCAGTTTTCATATAAGGATGAAGAGAATGGAAACAGAG
GAGGAAAGCAAAGGAACCGATTGGAGCCGATGGATACCATATTTGTTAAACAAGTGAAGGAAGGAGGGCC
GGCCTTCGAGGCTGGCTTATGCACAGGTGACAGAATTATAAAAGTCAATGGAGAAAGTGTCATAGGAAAG
ACCTATTCCCAAGTAATTGCTCTCATTCAGAACAGTGACACCACCCTGGAGCTCAGTGTAATGCCCAAGG
ATGAAGACATTCTCCAAGTGCTACAATTTACAAAGGATGTTACAGCACTGGCATATTCTCAAGATGCATA
CCTGAAAGGCAATGAAGCTTACAGCGGCAATGCCCGTAATATACCTGAACCTCCACCAGTTTGCTATCCC
TGGTTGCCATCGACTCCTTCAGCTACTGCACAGCCGGTGGAAACATGCCCTCCTGATTCATTACCGAACA
AACAGCAAACCAGTGCACCAGTCCTCACACAACCTGGCAGGGCCTATAGAATGGAAATACAAGTGCCTCC
GTCACCAACAGATGTTGCAAAGTCAAACACAGCAGTGTGTGTTTGCAATGAGAGTGTGAGGACCGTCATT
GTGCCTTCTGAGAAGGTGGTAGACTTGCTAGCCAATAGAAACAACCCTTCAGGTCCTTCACATAGAACTG
AAGAGGTAAGGTATGGTGTAAATGAGCAGGCCTCTACAAAAGCAGCATCGAGAACAACGTCACCGGCATC
AGTTCCCACTGCCCATCTGATTCATCAGACCACAGGCTCCAGATCCTTAGAACCTTCTGGAATTTTACTT
AAATCTGGAAATTACAGTGGACATTCAGAAGGAATCTCAAGCAGTAGATCACAAGCCGTGGATTCCCCTC
CTGTGTCTGTCAATCACTACTCTGCAAACTCCCATCAGCACATAGACTGGAAAAATTATAAGACTTACAA
AGAATACATTGATAATAGGAGATTGCACATAGGTTGTCGGACAATCCAGGAAAGACTGGACAGCTTACGA
GCTGCATCTCAGAGCGCAGCAGACTATAACCAGGTGGTACCCACCCGCACTACTCTGCAGGTACGACGTC
GAAGCACCTCTCATGACCGAGTGCCCCAGTCTGTTCAGATCCGGCAGCGCAGTGTATCTCAGGAAAGACT
GGAAGATTCTGTGCTGATGAAGTATTGCCCAAGGAGCGCGTCTCAAGGAGCGCTGACCTCACCGCCTGTT
AGCTTCAATAATCATAGAACTCGTTCGTGGGATTACATAGAGGGACAGACGGAAGCCACAGCCACTGTCA
ATTCTGAAAGTCAGATACCTGACTCAAATGGGGAACGAAAACAGACTTACAAGTGGAGTGGCTTCACTGA
GCAGGATGATAGACGCGGTATTCACGAAAGACCCAGGCAGCAAGAAATGCACAAGCCTTTCCGAGGCTCA
AATTTGACTGTGGCTCCCGTTGTTAATTCTGATAACAGGCGCCTGGTTGGGAGAGGGGTAGGACCTGTCT
CACAGTTTAAAAAAATCCCGCCGGACCTAAGACCACCACATTCCAACAGAAATTTTCCAACCACGACTGG
GGTGTCACTTCAACGGGGTATTGCACAAGACAGGTCCCCACTCGTGAAAGTCCGAAGTAATTCTCTGAAA
GTGCCACCTCCACCTGTGTCAAAACCGTCCTTCAGCCAGCACTCACTGGCTTCCATGAAAGACCAAAGGC
CTGTCAATCATCTGCATCAACACAGTGTGCTGAGTCAGCAGACGCAGTTCAGATCCGAAAGCACCTTCGA
GCACCAGCTGGAGACTGAGGTATCCTCGTGTCTACCAGGAACTTCTGCCAAGACCAGTCCACAGCTCAGT
GAGAATTTGGGAACATCAGACTTAGAACTACCTGCCATCCCAAGGAATGGAGATATAAACTTACAGGAGG
CTGAAATTCAGCAGCCAGATGTGCTAGATAATAAAGAATCTGTCATTCTAAGAGAGAAACCTCAGTCTGG
CCGCCAGACGCCACAGCCTTTAAGACATCAGTCTTACATCCTGGCAGTGAATGACCAGGAGACCGGGTCA
GACACCACCTGCTGGCTGCCAAACGATGCACGCAGAGAGGTCCATATAAAAAGAATGGAGGAGAGGAAAG
CTTCAAGCACCAGCCCTCCTGGTGATTCCCTGGCATCCATCCCTTTTATAGATGAGCCAACCAGTCCTAG
CATCGACCACGAGATTGCACATATTCCTGCATCTGCTGTCATCTCCGCCTCTACTGCCCACGTCCCCTCC
ATTGCAACTGTTCCCCCCAGCCTCACGACCTCTGCTCCTTTGATTCGACGTCAGCTCTCCCACGACCAAG
AATCTGTTGGACCTCCCAGCCTGGATGGCCAGCACAGCTCAAAGACAGAAAGATCCAAATCCTACGATGA
AGGACTGGATGATTATAGAGAAGATGCAAAACTATCCTTTAAGCACGTGTCTAGCCTGAAGGGAATCAAG
ATTACAGACAGCCAGAAGTCATCAGAAGACTCTGGATCGAGAAAGGGTTCTTCCTCCGAGGTCTTCAGTG
ATGCAGCCCGAGAAGGCTGGCTCCAGTTCCGACCACTGGTCACCGATAAGGGCAAGAGAGTTGGTGGCAG
TATTCGGCCATGGAAACAGATGTATGTTGTCCTTCGGGGCCACTCACTTTACTTGTACAAAGACAGAAGA
GAGCAGACAACGCCATCAGAAGAAGAGCAGCCCATCAGTGTCAACGCTTGCCTGATAGACATCTCGTACA
GTGAGACCAAGAGGCGGAATGTATTCCGACTTACCACGTCTGACTGTGAATGCTTGTTCCAGGCTGAAGA
CAGAGATGATATGTTATCATGGATCAAGACTATTCAGGAAAGTAGCAACCTAAATGAAGAGGACACTGGA
GTTACCAACAGAGATCTAATTAGTCGAAGAATCAAAGAGTATAACAGTCTGCTGAGCAAAACAGAACAGT
TGCCAAAAACACCTCGCCAGAGTCTTAGCATCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAAGAC
TCAAAGCCCACATTCTCCAAAGGAGGAGTCCGAGAGGAAGCTTCTCAGTAAAGATGATACCAGTCCCCCT
AAAGACAAAGGTACTTGGAGAAGAGGCATTCCAAGCATCGTGAGGAAGACGTTTGAGAAAAAGCCGGCCG
CCACAGGGACCTTTGGAGTTAGGCTGGATGACTGCCCACCCGCCCACACCAACAGGTACATTCCATTAAT
CGTTGACATATGTTGCAAATTAGTTGAAGAAAGGGGTCTTGAATATACAGGTATTTACCGAGTTCCCGGC
AACAACGCAGCCATTTCAAGCATGCAGGAAGAACTCAACAAGGGGATGGCAGACATTGACATACAAGATG
ATAAATGGCGAGATTTGAATGTGATAAGCAGTTTACTAAAATCCTTCTTCAGAAAGCTCCCTGAGCCTCT
CTTTACGAATGACAAATATGCTGACTTCATTGAGGCCAATCGCAAAGAGGACCCACTGGATCGGCTACGA
ACATTAAAGAGACTAATTCATGACCTGCCTGAGCATCATTTTGAGACGCTCAAGTTTCTCTCGGCTCATC
TGAAGACAGTGGCAGAGAACTCAGAAAAGAATAAGATGGAACCCAGAAACCTAGCGATCGTTTTTGGTCC
AACTCTAGTGAGAACATCAGAAGACAACATGACCCACATGGTCACGCACATGCCTGACCAGTATAAGATC
GTAGAGACTCTCATCCAGCATCATGACTGGTTTTTCACAGAAGAAGGTGCCGAAGAGCCTCTTACAGCAG
TGCAGGAGGAGAACACAGTAGACTCCCAGCCAGTACCAAACATAGATCATTTGCTCACCAACATTGGAAG
GACAGGCGTGCTCCCTGGAGATGTATCAGATTCAGCTACTAGTGACTCCGCAAAATCTAAGGGCTCCTGG
GGATCCGGGAAGGATCAGTATAGCAGGGAACTGCTTGTATCCTCCATCTTCGCAGCTGCCAGTCGCAAGA
GGAAAAAGCCGAAAGAGAAAGCCCAGCCCAGCAGCTCTGAGGATGAGCTGGACAGTGTGTTCTTTAAGAA
GGAGAATACGGAACAGAGTCACAGTGAGATTAAAGAAGAGTCCAAAAGAGAAAGCGAGACATCAGGTAGC
AAGCAGAGGGTCGTCGTTGCCAAAGAAAGTAACACTAAGAAAGACTCGGGTACAACGAAGGAGGAGAAGA
AGATACCGTGGGAAGAACCATCCCCACCACACAGCTCCAAACGCAACAGGTCACCAACCCTCAGCTGTCG
CCTGGCCATGCTGAAAGAGGGCCCCAGATCACTTCTGACACAGAAGCCCCACTGTGAAGAGACAGGGTCT
GACTCAGGCACTCTGCTTAGCACGTCTTCCCAGGCTTCGCTCTTGAGGTCCTCCACTAAAAAATCCACCA
GTCCGGAAACCAAGCACAGTGAGTTTTTGTCCATTGCTGGTACCACCACCTCGGATTATTCTACCACATC
ATCCACTACCTACTTGACCAGCCTGGACTCCAGCCGCCTGAGCCCTGAGGTGCAGTCAGTGGCCGAGAGC
AAGGGGGATGAGGCTGATGACGAGAGGAGCGAGCTGGTGAGTGAAGGACGGCCAGTGGAGACCGACAGTG
AGAGTGAGTTCCCCGTGTTTCCCGCCACCCTGACTTCAGACCGGCTGTTCCGAGGAAAATTCCAGGATGT
TGCCAGGGTGAGCCGCAGGAACTCCGAAGGCAGTGAGGCCAGCTGCACCGAGGGAAGCCTCACACCGAGT
TTAGACAGCCGCAGGCAGCAGTTCAGCTCCCATAGACTGATCGAATGCGACACACTGTCCAGGAAGAAGT
CTGCTCGCTTCAAGTCGGACAGTGGGAGCCCCGGAGACACCAGGACTGAGAAAGAAACTCCTGCCCTGGC
CAAGATGTTTGACGTCATGAAGAAAGGCAAGTCGACAGGGAGCCTCCTCACACCCAGCAGAAGTGAATCC
GAAAAGCAGGAGGCCACTTGGAAAACAAAAATAGCAGATCGTTTAAAACTGAGACCCAGGGCACCCGCAG
ACGACATGTTTGGAGTTGGGAATCAAAAACCTACTGCTGAGACGGCGAAAAGGAAAAACATCAAACGCAG
ACACACGCTTGGAGGCCACAGAGATGCTACTGAGATCAGCGTCCTGAGTTTCTGGAAAGCTCATGAGCAG
AGTGCAGACAAAGAGTCTGAACTTTCGGCTGTGAATCGGTTAAAACCCAAATGCTCAGCCCAGGACCTTT
CCATCTCAGACTGGCTCGCCAGGGAGCGCGTCCGCACCAGTGCCTCCGACCTTAGCAGAGGCGAAGGCCT
CGAGCCCCAGGCTGAGAGCCCCAGCGTGCTGGGAACACCCATCAGCACCCACTCACCACCGTCTCAGCAG
CCCGAAGCAAGGGTTGCTGCTACCAGCACCTTGGCTTCAACCAGCCAGTCCCCTCTTTTCACACCACCAC
AGTCACCTGACCAAATAAACAGAGAAAGCTTCCAGAACATGAGCCAAAATGCTAGTTCTACAGCTAACAT
CCACCCCCACAAACAGTCTGAAAGTCCAGACACTAAAGCAGAGACTCCTCCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215805 representing NM_001128084
Red=Cloning site Green=Tags(s)

MATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLR
HFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGK
TYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYP
WLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVI
VPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILL
KSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLR
AASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPV
SFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGS
NLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLK
VPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLS
ENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGS
DTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPS
IATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIK
ITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRR
EQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTG
VTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPP
KDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPG
NNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLR
TLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKI
VETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSW
GSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGS
KQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGS
DSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAES
KGDEADDERSELVSEGRPVETDSESEFPVFPATLTSDRLFRGKFQDVARVSRRNSEGSEASCTEGSLTPS
LDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSES
EKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQ
SADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQ
PEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001128084
ORF Size 5862 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001128084.2, NP_001121556.2
RefSeq Size 6974 bp
RefSeq ORF 5868 bp
Locus ID 71435
Cytogenetics 2 A3
Summary Functions as a GTPase-activating protein (GAP) for RHOA and CDC42. Downstream partner of ARF1 which may control Golgi apparatus structure and function. Also required for CTNNA1 recruitment to adherens junctions (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001128084" in other vectors (1)
SKU Description Size Price
MR215805 Arhgap21 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 21 (Arhgap21), transcript variant 1 10 ug
$1,643.00

file-alt Citations

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