Snrpf (NM_027246) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG215801
Snrpf (tGFP-tagged) - Mouse small nuclear ribonucleoprotein polypeptide F (Snrpf), (10ug)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Snrpf
Synonyms 2010013O18Rik; sm-F; SMF; snRNP-F
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215801 representing NM_027246
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTCTACCCCTCAACCCCAAGCCCTTTCTCAATGGGCTGACAGGGAAGCCAGTGATGGTGAAACTTA
AGTGGGGCATGGAGTACAAGGGCTACCTGGTCTCCGTGGATGGCTACATGAACATGCAGCTTGCAAATAC
AGAAGAGTACATAGATGGGGCGCTGTCTGGACATCTGGGCGAAGTTCTAATAAGGTGTAATAATGTCCTC
TACATCAGAGGCGTTGAAGAAGAAGAGGAAGATGGGGAAATGAGAGAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215801 representing NM_027246
Red=Cloning site Green=Tags(s)

MSLPLNPKPFLNGLTGKPVMVKLKWGMEYKGYLVSVDGYMNMQLANTEEYIDGALSGHLGEVLIRCNNVL
YIRGVEEEEEDGEMRE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027246
ORF Size 258 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027246.1, NP_081522.1
RefSeq Size 869 bp
RefSeq ORF 261 bp
Locus ID 69878
UniProt ID P62307
Cytogenetics 10 C2
Summary Plays role in pre-mRNA splicing as core component of the SMN-Sm complex that mediates spliceosomal snRNP assembly and as component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. Is also a component of the minor U12 spliceosome. As part of the U7 snRNP it is involved in histone 3'-end processing.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027246" in other vectors (4)
SKU Description Size Price
MC211018 Snrpf (untagged) - Mouse small nuclear ribonucleoprotein polypeptide F (Snrpf), (10ug) 10 ug
$165.00
MR215801 Snrpf (Myc-DDK-tagged) - Mouse small nuclear ribonucleoprotein polypeptide F (Snrpf) 10 ug
$289.00
MR215801L3 Lenti ORF clone of Snrpf (Myc-DDK-tagged) - Mouse small nuclear ribonucleoprotein polypeptide F (Snrpf) 10 ug
$450.00
MR215801L4 Lenti ORF clone of Snrpf (mGFP-tagged) - Mouse small nuclear ribonucleoprotein polypeptide F (Snrpf) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.