Gabrg3 (NM_008074) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG215787
Gabrg3 (tGFP-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor subunit gamma 3 (Gabrg3), (10ug)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gabrg3
Synonyms B230362M20Rik; Gabrg-3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215787 representing NM_008074
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCAAAGCTGCTGCTTCTCCTCTGCCTGTTCTCGGGCTTGCATGCTCGGTCCAGGAGGGTAGAAG
AAGATGAGAATGAAGACTCCCCATCAAACCAGAAGTGGGTCTTGGCACCCAAATCACAAGACACCGATGT
AACGCTGATTCTCAACAAATTGTTGAGAGAATATGACAAAAAGCTGAGACCGGACATTGGAATAAAACCA
ACTGTGATCGATGTGGACATTTATGTTAACAGCATTGGTCCTGTGTCATCCATAAACATGGAATACCAAA
TTGATATATTTTTTGCTCAAACCTGGACAGACAGTCGCCTTCGATTCAACAGCACAATGAAAATACTAAC
TCTGAACAGCAACATGGTGGGCTTGATATGGATTCCAGACACCATCTTTCGAAATTCTAAAACAGCGGAG
GCTCACTGGATCACCACACCCAACCAGCTCCTCAGAATCTGGAATGATGGGAAAATCCTTTACACATTAA
GGCTCACTATCAATGCAGAGTGCCAGCTGCAACTGCATAACTTCCCTATGGATGCGCATGCCTGCCCCTT
GACCTTCTCTAGCTATGGCTACCCCAAAGAAGAAATGATTTATCGTTGGAGGAAAAATTCAGTTGAGGCA
GCTGATCAGAAATCATGGCGGCTCTATCAGTTTGACTTCATGGGCCTCAGAAACACTACAGAAATCGTGA
CAACATCTGCAGGTGATTATGTTGTCATGACTATCTATTTTGAACTGAGTCGAAGAATGGGATACTTCAC
GATCCAGACGTATATCCCCTGCATACTGACTGTGGTTCTGTCCTGGGTGTCATTTTGGATAAAAAAGGAT
GCGACACCAGCAAGAACAACATTAGGCATCACCACGGTGCTAACCATGACCACACTCAGCACCATTGCCA
GGAAGTCTCTGCCTCGGGTGTCCTATGTCACTGCCATGGACCTCTTTGTGACTGTGTGCTTCTTGTTTGT
CTTTGCCGCACTGATGGAGTATGCTACACTCAACTACTATTCAAGCTGTCGAAAGCCAACCATCAGGAAG
AAAAAAACATCGTTATTACATCCAGATTCCACAAGATGGATTCCTGATCGAATAAGCCTTCAAGCACCCT
CTAATTACTCTCTACTGGACATGAGGCCCCCACCACCTGTGATGATCACGTTAAACAATTCCATGTACTG
GCAGGAATTTGAGGACACCTGTGTCTATGAGTGTCTGGATGGCAAAGACTGCCAGAGCTTCTTCTGCTGC
TATGAGGAGTGCAAGTCTGGCTCCTGGAGGAGAGGCCGCATCCACATTGATGTCTCTGAGCTGGACTCCT
ACTCTCGGGTCTTCTTCCCGACATCCTTCCTGCTGTTCAACCTGGTCTATTGGGTTGGATACCTGTATCT
T


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215787 representing NM_008074
Red=Cloning site Green=Tags(s)

MAAKLLLLLCLFSGLHARSRRVEEDENEDSPSNQKWVLAPKSQDTDVTLILNKLLREYDKKLRPDIGIKP
TVIDVDIYVNSIGPVSSINMEYQIDIFFAQTWTDSRLRFNSTMKILTLNSNMVGLIWIPDTIFRNSKTAE
AHWITTPNQLLRIWNDGKILYTLRLTINAECQLQLHNFPMDAHACPLTFSSYGYPKEEMIYRWRKNSVEA
ADQKSWRLYQFDFMGLRNTTEIVTTSAGDYVVMTIYFELSRRMGYFTIQTYIPCILTVVLSWVSFWIKKD
ATPARTTLGITTVLTMTTLSTIARKSLPRVSYVTAMDLFVTVCFLFVFAALMEYATLNYYSSCRKPTIRK
KKTSLLHPDSTRWIPDRISLQAPSNYSLLDMRPPPPVMITLNNSMYWQEFEDTCVYECLDGKDCQSFFCC
YEECKSGSWRRGRIHIDVSELDSYSRVFFPTSFLLFNLVYWVGYLYL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008074
ORF Size 1401 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008074.3
RefSeq Size 1673 bp
RefSeq ORF 1404 bp
Locus ID 14407
UniProt ID P27681
Cytogenetics 7 33.48 cM
Summary GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008074" in other vectors (4)
SKU Description Size Price
MC216254 Gabrg3 (untagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit gamma 3 (Gabrg3), (10ug) 10 ug
$503.00
MR215787 Gabrg3 (Myc-DDK-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit gamma 3 (Gabrg3) 10 ug
$289.00 $457.00
MR215787L3 Lenti ORF clone of Gabrg3 (Myc-DDK-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit gamma 3 (Gabrg3) 10 ug
$757.00
MR215787L4 Lenti ORF clone of Gabrg3 (mGFP-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit gamma 3 (Gabrg3) 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.