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Arhgap8 (NM_001164628) Mouse Tagged ORF Clone

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SKU
MG215772
Arhgap8 (tGFP-tagged) - Mouse Rho GTPase activating protein 8 (Arhgap8) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Arhgap8
Synonyms 3110043J09Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215772 representing NM_001164628
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGGCCTGGACCCCACACTGAGCACAAGTCACCCATTCTATGATGTGGCCAGACACGGCATCCTGC
AGGTGGCAGGGGATGACCGCCAGGGGAGACGCATCTTCACTTTCAGCTGCTGCCGGCTGCCACCCTTGCA
CCAGCTCAACCACCAGCGCCTGCTGGAGTATCTGAAGTACACACTGGACCAGCATGTGGAGAACGACTAT
ACTATTGTCTACTTTCACTATGGCCTCAGCAGCCAGAACAAACCATCCCTAGGCTGGCTCCAGAACACCT
ACAAGGAGTTTGACCGGAAGTACAAGAAGAACCTGAAGGCACTGTACGTTGTACACCCCACCAGCCTCAT
CAAAGCACTGTGGAACATCTTCAAGCCCCTCATCAGTCACAAGTTTGGGAAAAAAGTCACCTACTGCAGC
AACCTGCGTGAGCTCCGAGAACACCTTCAGTGCGACCAGCTGCTCATCCCCCCGGAAGTAGTGCGGTATG
ACGAGAAACTGCAAAACCTGCACAAAGGTCAACCGCCCCCGCCCACCAAGACACCGCCACCTCGGCCGCC
TCTGCCCACCCAGCAGTTTGGCGTCAGCCTGCAATACCTCAGAGACAAAAATCAAGGTGAACTCATCCCC
CCGGTGCTGCGTTGGACGGTGACATATCTGAGAGAAAAAGGACTGCGCACTGAGGGCCTGTTCCGGAGAT
CGGCCAGCGCCCAGACTGTCCGTCAGGTGCAGCGGCTATATGATCAAGGGAAGCCCGTGAACTTTGATGA
TTATGGAGACATGCACCTCCCAGCCGTGATCCTGAAGACATTTCTCCGAGAGCTGCCCCAGCCACTGCTG
ACCTTCCAAGCCTACGAGCAGATTCTCGGGATCACCAGTGTGGAGAGCAGCCTTCGAGTGACCCACTGCC
GCCTGATCCTGCGGAGCCTCCCAGAACACAACTATGCCGTACTCCGCTACCTCATGGGCTTCCTGCATGA
AGTGTCTCTGGAGAGCATTTCAAACAAGATGAACAGCTCTAACCTGGCGTGCGTGTTTGGATTGAACTTG
ATCTGGCCATCCCAGGGGGTGGCTTCCCTGAGCGCCCTGGTACCTCTGAACTTGTTCACAGAGCTGCTGA
TAGAGTACTATGACAAAGTCTTCAGTTGCCAGGAGGCCCCTGGGGAGCACACCCGGGATACTGTGGAAGT
ACAGCAGGCTGGCCCTGTTACCAAAGAATTTATGAAGACGGGCACTCCCCGGGCCTCACCATACCTGTCC
CGCCTCCGTATCTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215772 representing NM_001164628
Red=Cloning site Green=Tags(s)

MAGLDPTLSTSHPFYDVARHGILQVAGDDRQGRRIFTFSCCRLPPLHQLNHQRLLEYLKYTLDQHVENDY
TIVYFHYGLSSQNKPSLGWLQNTYKEFDRKYKKNLKALYVVHPTSLIKALWNIFKPLISHKFGKKVTYCS
NLRELREHLQCDQLLIPPEVVRYDEKLQNLHKGQPPPPTKTPPPRPPLPTQQFGVSLQYLRDKNQGELIP
PVLRWTVTYLREKGLRTEGLFRRSASAQTVRQVQRLYDQGKPVNFDDYGDMHLPAVILKTFLRELPQPLL
TFQAYEQILGITSVESSLRVTHCRLILRSLPEHNYAVLRYLMGFLHEVSLESISNKMNSSNLACVFGLNL
IWPSQGVASLSALVPLNLFTELLIEYYDKVFSCQEAPGEHTRDTVEVQQAGPVTKEFMKTGTPRASPYLS
RLRIS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001164628
ORF Size 1275 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001164628.1, NP_001158100.1
RefSeq Size 1519 bp
RefSeq ORF 1278 bp
Locus ID 73167
UniProt ID Q9CXP4
Cytogenetics 15 E2
Summary GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001164628" in other vectors (3)
SKU Description Size Price
MR215772 Arhgap8 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 8 (Arhgap8), transcript variant 2 10 ug
$289.00 $457.00
MR215772L3 Lenti ORF clone of Arhgap8 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 8 (Arhgap8), transcript variant 2 10 ug
$757.00
MR215772L4 Lenti ORF clone of Arhgap8 (mGFP-tagged) - Mouse Rho GTPase activating protein 8 (Arhgap8), transcript variant 2 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.