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Anapc1 (NM_008569) Mouse Tagged ORF Clone

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SKU
MG215771
Anapc1 (tGFP-tagged) - Mouse anaphase promoting complex subunit 1 (Anapc1), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,833.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Anapc1 or NM_008569"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Anapc1
Synonyms 2610021O03Rik; AI047775; AI853536; Apc1; AW547281; Mcpr; tsg24
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215771 representing NM_008569
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGAACTTCTCTGAAGAAAGGGCAACGATGATTGCAGCCGGGGATTTGCAGGAATTTGTTCCTTTTG
GCCGAGACCACTGCAAGCACCACCCTAACGCTCTGAACCTTCAGCTTCGTCAGCTGCAGCCAGCCTCTGA
GTTATGGTCTTCTGATGGTGCAGCTGGCTTGGTGGGATCTCTTCAGGAGGTTACAATCCATGAGAAACAG
AAGGAAAGCTGGCAGTTAAGAAAAGGTGTGAGTGAGATTGGAGATGCAGCTGACTACGATGAAGAGCTCT
ATGTGGCTGGGAACATGGTTATCTGGAGCAAAGGGAGTAAGAGCCAGGCACTGGCAGTGTACAAAGCGTT
CACAGTGGACAGCACTGTCCAGCAGGCATTGTGGTGTGACTTCATTATATCACAGGATAAGTCTGAAAAG
ATCTATAAGAGCCATGAACTAGAGAAATGCATATGTATATTACAGAGTTCATGTATGAACATGCATAGCA
TAGACGGGAAGGATTATATAGCTTCCTTACCTTTTCAGGTGGCAAATGTTTGGGCCACTAAATATGGCTT
GCTGTTTGAACGATGCAGTTCTTCACATGAGGTACCTCCAAGTCTACCTAGAGAACCTTTGCCTACTATG
TTCAGCATGCTGCACCCACTGGATGAGATAACTCCACTTGTTTGTAAATCCGGAAGTCTTTTTGGCTCAT
CACGGGTACAGTATGTTGTAGATCCAGCAGTGAAAATTGTGTTTCTGAACATTGACCCCTCCATTGTCAT
GACCTATGATGCTGTTCAGAATGTGCATTCTGTGTGGACTCTGCGGAGGGTAAAGCCAGAGGAAGAGAAT
GCTGTTTTAAAGTTCCCTGAGCAGGCAGGGACCCTGCAGAATGCAACTACTAGCAGCTCCCTGACAGCAC
ATCTCAGAAGCCTCTCCAAAGGCGAATCCCCTGTGGCTTCGCCGTTCCAGAATTACTCTTCCATTCACAG
CCAGAGTCGCTCAACCTCTTCGCCCAGTCTACACTCTCGGTCGCCTTCCATTTCCAACATGGCGGCTCTA
AGTCGTGCTCACTCTCCTGCCTTAGGTGTGCACTCTTTCTCAGGGGCGCAAAGATTCAACCTTTCAAGCC
ACAGTCAGTCACCAAAGAGACACAGCATTTCTCACTCTCCGAGTGGCAGTTTCAACGACTCATTTTTGGC
ACCAGAAACAGAGCCAATTGTTCCTGAGCTGTGCATTGATCACTTATGGACTGAGACACTTCCGAATATA
AGAGAGAAAAATTCTCAAGCCTCCAAGGTATTTATAACGACTGACCTGTGTGGACAGAAGTTCTTGTGCT
TTTTAGTGGAGGCCCAGCTTCAGTTACGCTGTGTAAAGTTTCAAGAGAGTAATGACAAGACTCAGCTTAT
CTTTGGCTCTGTCACCAACATACATGCAAAAGATGCAGCCCCAGTGGAGAAGATACACACCATGCTGGTC
CTAGAAGGCAACGGGAACCTGGTGCTGTACACAGGAGTGGTGCGGGTGGGGAAAGTCTTTATCCCTGGAC
TTCCGGCTCCTTCCCTGACAATGTCCAACATGATGCCTCGGCCCAGTACCCCGCTCGACGGTGTTGGTAC
TCCAAAGCCTCTGAGTAAATTGCTTGGATCGATGGATGAGGTGGTTCTGTTGTCTCCAGTTCCAGAATTG
AGGGATTCTTCAAAACTTAATGATTCTCTCTACAATGAGGATTGCACATTCCAGCAGCTTGGAACTTACA
TTCATTCTGTGCGAGACCCTGTTCATAACAGAGTCACTCTAGAACTGAGCAATGGCTCCATGGTTCGGAT
CACTATCCCTGAAGTGGCCACCTCGGAATTAGTACAAACGTGTCTGCAAGCAATTAAGTTCATCCTGCCA
AAAGAAGTAGCCATTCAGGTGCTCGTCAAGTGGTACAATGTGCACAGTGCTCCAGGAGGACCCAGCTGTC
ACTCAGAGTGGAGTTTATTTGTGATTTGTCTCTTGAACATGATGGGTTATAACACAGACCGCTTAGCATG
GACCCGAAGTTTTGACTTTGAAGGATCACTTTCCCCAGTCATTGCACCCAAAAAAGCAAGGCCTTCTGAT
ACTGGATCTGACGAGGACTGGGAGTACTTACTGAACTCAGAGTACCATCGCAATGTTGAGTCTCATCTTT
TGAACAAATCTCTATGTTTGACTGCTTTGGAAGTTTCAAATGCAAAGGATGAAGATTTTTCGCAGAACCT
CAGTCTGGATTCTTCTACCCTTCTCTTTGCTCACATTCCTGCAATTTTCTTTGTTCTTCACCTGGTTTAT
GAAGAGCTCAAGTTGAATACTCTAATGGGAGAAGGAATTTGTTCTCTTATCGACCTCCTTGTTCAGTTGG
CAAGGGACTTAAAATTGGACTCTTACTTGGACCATTACTATCGAGATTCCCCAACTCTTGTCAAAACCAC
TGGACAAGTATGCACCATTGACCAAGGTCAGATGGGATTTATGCATCATCCCCCATTTTTTACTTCTGAG
CCACCAAGTATTTATCAGTGGGTGAGTTCATGCCTGAAGGGTGAAGGGATGCCACCCTATCCTTACCTTC
CTGGGATTTGCGAGAGGAGCAGGCTAGTGGTCTTGAGCATTGCCCTCTATACCCTTGGTGATGAGAGCTG
TGTGTCTGATGAAACTTGCCAGTACTTATCCAAAGTAACCTCAACTCCCCAAAAGCCACAAGCAGAGCAA
GAAGAAAACAGGTTTACTTTCCGGCATTCTGCTTCCGTTTCTGTTCTGGCCGAGAGATTAGTTGTCTGGA
TGGCCAGTGTAGGATTCACTTTAAGAGATCTGGAGACTCTTCCCTTTGGGATTGCTCTTCCCATCAGAGA
TGCGATTTACCACTGTCGGGAGCAGCCTGATTCAGATTGGTCAGAAGCGGTCTGTCTCTTGATTGGACGT
CAGGATCTTTCCAAGCAGGCTTGTGAAGGAAATTTACCCAGAGGCAAATCTGTGCTCTCATCAGAGGTGT
CTTCAGGAACTGAGGCAGAGGAGGAAGATGATGGCATGAATGACTTGAACCATGAGGTTATGTCATTAAT
ATGGAGTGAAGATTTACGGGTGCAGGATGTGCGAAGGCTGCTTCAAAGTGCACAGCCTGTCCGTGTCAAT
GTGGTGCAGTACCCAGAACTCAGTGACCATGAGTTCATTGAAGAAAAGGAAAACAGACTGCTCCAGTTGT
GTCAGCGAACTATGGCCCTTCCAGTAGGACGAGGAATGTTTACCTTGTTCTCATATCATCCTGTTCCAAC
AGAGCCGTTGCCTGTTCCTAAATTGAATCTGACAGGGCGAGCCCCTCCACGAAACACAACTGTAGATCTT
AATAGCGGAAACATCGACGTGCCTCCCAACATGGCCAGCTGGGCCAGCTTTCATAATGGTGTGGCTGCCG
GCCTGAAGATAGCCCCAGCCTCCCAGATAGACTCAGCTTGGATCGTTTACAACAAGCCAAAGCATGCTGA
GTTAGCCAATGAGTATGCCGGCTTTCTCATGGCCCTGGGTCTGAATGGGCACCTTACCAAGCTGGCTACT
CTCAATATCCATGACTATTTGACCAAGGGCCATGAAATGACAAGCATTGGATTGCTACTTGGTGTTTCTG
CTGCAAAGCTTGGCACCATGGACATGTCAATTACCCGGCTCCTCAGCATTCACGTTCCTGCTCTCTTACC
CCCGACATCCACAGAGCTTGACGTGCCTCACAATGTCCAAGTGGCGGCTGTGGTTGGCATTGGCCTTGTG
TATCAGGGCACAGCTCACAGACACACTGCGGAAGTCCTGTTGGCTGAAATAGGGCGGCCCCCTGGTCCAG
AAATGGAATACTGCACTGACAGAGAGTCGTACTCCTTAGCTGCTGGCCTGGCCCTGGGCATGGTTTGCTT
GGGGCATGGCAGCAATTTGATTGGCATGTCTGATCTCAATGTGCCTGAGCAGCTGTATCAGTACATGGTT
GGAGGCCATAGGCGATTTCAAACTGGAATGCACAGGGAGAAACATAAGTCTCCAAGTTATCAGATCAAAG
AAGGAGACACCATAAACGTGGATGTGACTTGTCCCGGTGCTACTCTGGCGTTGGCTATGATCTACTTAAA
AACCAATAACAGGTCCATTGCTGATTGGCTGCGTGCTCCTGATACCATGTATTTGCTAGACTTTGTGAAA
CCAGAATTCCTTTTGCTTAGGACACTTGCTCGGTGCTTGATTTTGTGGGATGATATCTTACCAAATTCCA
AGTGGGTTGACAGCAATGTTCCTCAGATTATAAGAGAAAATAGTATCTCTCTGAGTGAAATTGAATTGCC
TTGTTCAGAGGACTTGAATTTGGAAACCTTGTCGCAAGCACACGTCTACATCATTGCAGGAGCCTGCTTG
TCTCTAGGGTTTCGATTTGCTGGCTCAGAAAACTTATCAGCATTTAGCTGTCTGCATAAATTTGCAAAAG
ATTTTATGAATTATTTATCTGCACCCAATGCTTCTGTAACAGGGCCCTATAACCTCGAAACCTGCCTGAG
TGTGGTCCTGCTGTCTCTTGCCATGGTGATGGCTGGCTCTGGGAACCTGAAGGTGTTGCAGCTCTGTCGC
TTTCTGCACATGAAGACTGGTGGAGAGATGAACTATGGCTTCCACTTGGCCCACCACATGGCCCTGGGCC
TTCTCTTTTTGGGAGGAGGAAGGTACTCTTTGAGCACATCCAACTCATCCATTGCTGCCCTTCTCTGCGC
CCTTTACCCACATTTCCCAGCCCACAGCACTGACAACCGGTATCATCTCCAGGCCCTTCGGCACCTCTAT
GTGCTAGCTGCAGAACCAAGGCTCCTGGTACCTGTGGATGTGGATACAAACACACCCTGCTATGCCCTTA
TAGAAGTTACTTACAAGGGCACTCAGTGGTATGAACAGACCAAAGAAGAACTGATGGCTCCAACCCTTCT
TCCAGAACTTCATCTTTTAAAGCAGATGAAAGTTAAAGGGCCAAGATACTGGGAACTGCTCATAGATTTA
AGCAAGGGAGAACAGCACTTGAGGTCTATTCTTTCGAAGGATGGAGTTTTATATGTAAAGCTCAGGGCAG
GACAGCTCTCCTACAAAGAAGACCCAATGGGGTGGCAGAGTTTGTTGGCACAAACTGTTGCTAATAGGAA
CTCTGAAGCCCGGGCTTTCAAGCCTGAAACAATTTCATCATTCACTTCTGATCCAGCACTTCTGTCATTT
GCTGAATATTTCTGCAAGCCGACTGTGAGCATGGGTCCAAAACAGGAGATTCTGGACCTCTTTTCTTCGA
TACTTTATGAGTGTGTTGCTCAGGAAACCCCAGAGATGTTGCCTGCCTACATAGCAATGGATCAGGCTCT
AAGAAGTCTTAAAAAGAGAGACATGTCAGACACATCTGATCTTTGGCAGATAAAGTTGATATTAGAATTT
TTCAGTTCCCGAAGCCACCAGGATAGGCAGCATACCTATCCTAAGCGAGGGCTCTTCATAAATTCTGAGT
TCCTACCTGTGGTGAAGTGCACTGTTGATGCTACCCTGGACCAGTGGCTACAAGCTGGCGGTGATGTGTG
TGTGCATGCCTACCTCAGTGGGCAGCCTGTTGAGAAGTCTCAGCTAAACATGCTGGCCTGCTTCCTTGTC
TACCACTCTGTGCCAGCACCACGACACTTGCCCCCCATGGGACTGGAAGGGAGCACAAGCTTTGCTGAAC
TCCTCTACAGGTTCCGACACCTGAAGATGCCCGTCCGGGCTTTGCTAAGACTGGCTCCTGTGCTGCTTGG
GAACCCACAGCCGATGGTCATG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215771 representing NM_008569
Red=Cloning site Green=Tags(s)

MSNFSEERATMIAAGDLQEFVPFGRDHCKHHPNALNLQLRQLQPASELWSSDGAAGLVGSLQEVTIHEKQ
KESWQLRKGVSEIGDAADYDEELYVAGNMVIWSKGSKSQALAVYKAFTVDSTVQQALWCDFIISQDKSEK
IYKSHELEKCICILQSSCMNMHSIDGKDYIASLPFQVANVWATKYGLLFERCSSSHEVPPSLPREPLPTM
FSMLHPLDEITPLVCKSGSLFGSSRVQYVVDPAVKIVFLNIDPSIVMTYDAVQNVHSVWTLRRVKPEEEN
AVLKFPEQAGTLQNATTSSSLTAHLRSLSKGESPVASPFQNYSSIHSQSRSTSSPSLHSRSPSISNMAAL
SRAHSPALGVHSFSGAQRFNLSSHSQSPKRHSISHSPSGSFNDSFLAPETEPIVPELCIDHLWTETLPNI
REKNSQASKVFITTDLCGQKFLCFLVEAQLQLRCVKFQESNDKTQLIFGSVTNIHAKDAAPVEKIHTMLV
LEGNGNLVLYTGVVRVGKVFIPGLPAPSLTMSNMMPRPSTPLDGVGTPKPLSKLLGSMDEVVLLSPVPEL
RDSSKLNDSLYNEDCTFQQLGTYIHSVRDPVHNRVTLELSNGSMVRITIPEVATSELVQTCLQAIKFILP
KEVAIQVLVKWYNVHSAPGGPSCHSEWSLFVICLLNMMGYNTDRLAWTRSFDFEGSLSPVIAPKKARPSD
TGSDEDWEYLLNSEYHRNVESHLLNKSLCLTALEVSNAKDEDFSQNLSLDSSTLLFAHIPAIFFVLHLVY
EELKLNTLMGEGICSLIDLLVQLARDLKLDSYLDHYYRDSPTLVKTTGQVCTIDQGQMGFMHHPPFFTSE
PPSIYQWVSSCLKGEGMPPYPYLPGICERSRLVVLSIALYTLGDESCVSDETCQYLSKVTSTPQKPQAEQ
EENRFTFRHSASVSVLAERLVVWMASVGFTLRDLETLPFGIALPIRDAIYHCREQPDSDWSEAVCLLIGR
QDLSKQACEGNLPRGKSVLSSEVSSGTEAEEEDDGMNDLNHEVMSLIWSEDLRVQDVRRLLQSAQPVRVN
VVQYPELSDHEFIEEKENRLLQLCQRTMALPVGRGMFTLFSYHPVPTEPLPVPKLNLTGRAPPRNTTVDL
NSGNIDVPPNMASWASFHNGVAAGLKIAPASQIDSAWIVYNKPKHAELANEYAGFLMALGLNGHLTKLAT
LNIHDYLTKGHEMTSIGLLLGVSAAKLGTMDMSITRLLSIHVPALLPPTSTELDVPHNVQVAAVVGIGLV
YQGTAHRHTAEVLLAEIGRPPGPEMEYCTDRESYSLAAGLALGMVCLGHGSNLIGMSDLNVPEQLYQYMV
GGHRRFQTGMHREKHKSPSYQIKEGDTINVDVTCPGATLALAMIYLKTNNRSIADWLRAPDTMYLLDFVK
PEFLLLRTLARCLILWDDILPNSKWVDSNVPQIIRENSISLSEIELPCSEDLNLETLSQAHVYIIAGACL
SLGFRFAGSENLSAFSCLHKFAKDFMNYLSAPNASVTGPYNLETCLSVVLLSLAMVMAGSGNLKVLQLCR
FLHMKTGGEMNYGFHLAHHMALGLLFLGGGRYSLSTSNSSIAALLCALYPHFPAHSTDNRYHLQALRHLY
VLAAEPRLLVPVDVDTNTPCYALIEVTYKGTQWYEQTKEELMAPTLLPELHLLKQMKVKGPRYWELLIDL
SKGEQHLRSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISSFTSDPALLSF
AEYFCKPTVSMGPKQEILDLFSSILYECVAQETPEMLPAYIAMDQALRSLKKRDMSDTSDLWQIKLILEF
FSSRSHQDRQHTYPKRGLFINSEFLPVVKCTVDATLDQWLQAGGDVCVHAYLSGQPVEKSQLNMLACFLV
YHSVPAPRHLPPMGLEGSTSFAELLYRFRHLKMPVRALLRLAPVLLGNPQPMVM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008569
ORF Size 5832 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008569.2, NP_032595.2
RefSeq Size 8967 bp
RefSeq ORF 5835 bp
Locus ID 17222
UniProt ID P53995
Cytogenetics 2 F1
Summary Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008569" in other vectors (2)
SKU Description Size Price
MC225074 Anapc1 (untagged) - Mouse anaphase promoting complex subunit 1 (Anapc1), (10ug) 10 ug
$1,798.00
MR215771 Anapc1 (Myc-DDK-tagged) - Mouse anaphase promoting complex subunit 1 (Anapc1) 10 ug
$1,633.00

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