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Cwf19l2 (NM_027545) Mouse Tagged ORF Clone

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SKU
MG215770
Cwf19l2 (tGFP-tagged) - Mouse CWF19-like 2 cell cycle control (S. pombe) (Cwf19l2), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,012.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cwf19l2
Synonyms 3230401L03Rik; 9030209E10
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215770 representing NM_027545
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCGTTTAGTGTAAGATTTGAAAGTGCTAGCAGTATCGAAGAGCGAAAAGAACAGACCCGTAATG
CCAGGGCCGAGGTGTTACGCCAGGCCAAACATAATTTTGAGAAAGAACAAAGGGGTGAAGAACGTAAGCG
ACTTCGGGATGAGGACACATGGATGCTTCCTGATGTGCATGAGCGAATTGAACAGTTCTCACAGGAACAT
TCTGAGAAGAAAAAGAAGAAAAAGGACAAGCATTCAAAAAAAGTGAAGAAAGAGAAGAAGAAGAAGAGGA
AGAAACAGAAATGTCAGAAACAGAGTGAGTCGACTGACAGCTCCGCAAGCTCTGAAGATGAGTGGGTTGA
GGCTGCTCCCTCCCAGATATCTGACAAAGAAAAGACCTGGAAAGTGAAAGACAAAAGGACAGAAGAAGAA
TGTGACAGCCACGATATTCAGCGTGATGAGTGGATGACTATTGATTTTATGTCCATTAAAACTGTGTCCT
CATCATCACTCAAAGCTGAAAAGGAAACTCTAAGACAAATAGAGCGGGAGAAAACCCAAGTGTTAGAGCA
GTCCAAACTGCTCGAAAGAGAATTGAATCCATACTGGAAGGACGGGGGGACAGGTCTCCCATCAAAAACC
TGTATACTTCCAGTTACTAAAGCTAAAGGAGTGGAGGATGGCGGATTAAGCTGGCTAAGAAAGTCTTGTC
AAAGAATGAAGGAACAAGCTCAAAAAGAAAACAGAAATTTTGAAGATATTGTAGCTGAAAAATACGGGTC
AATGGAAATATTTCAGTCAAAATTAAAAGAAGCTGAAAAAATTGCATACAAAAAAGAAGACTGTGGATGG
GAACGGTGGAGAAAGCCTACATATTCAGATAGAGCACAGTGTAGTCAAGCAAGTGGCACATCAGACTTAG
TGAAATGTAAAAATCTCTCAGAGGACAGACACCTGGAAATGGAGCCAGCAAATAGCAGCAACTATAAGTT
TAGTGGTCCTGACACAGGGAAGAGATCTGGGACTTTACAAACCTGTAGAAGGGAATCTGCCCTAAGAAAA
AACCAAGATTCTTCTGGAAATTTGAGATCTAAATTCTTAAGACCCTCTGATGAAGATGAATTGTCATTTC
ACAAGAGGAAAAATTTTGAATCATCTAGTTCATATTCTCCATTAGTGGCTCAGGCTTCTTTGCATTGTGA
CTTTCGAAAACTCACAGAGAACAGTGAAGAAAGTTCAGCATCCTGCAGTCGATCTGATAGGCGACAAGAA
AACAGGAAGCCTTCAGATAAAAAGCCATTGGAAACCTGGAGCTACAACGCCAACCAGCACTCTACAGGAG
GTAGAAGAGAACAGTTACAGGCTGAGAGCATGAGTTGTGATCCACCAGGAAGAGGACTTCAGCAAGACAT
GACATTGACAATCGCTGGTCCAGAGGCAGAGTCCACCTATATCTTAAATGTTGATGAAAAGAACAAGTTG
GGGGCCAAGATTATCAAGGCAGAAATGATGGGGAATATGGAATTAGCTGAACAACTTAAAGCCCAACTTA
AAGAGGCAAATAAATTTAAAGAAACACAGATGCCGGCAAAAAGATTGGGCGTAGAGCATGAAGATGAACA
AGAAGTAATCCTTATTCAAACAGATAAGTCTGGAAGAATGTGGCCTGTGAGCAGCCCTAGAGAAACCCTG
GATATGAAAGCAGAACGAAGGAAGAGAAAGAGAGTTTCAACCCATGAGGATAAAGAAAGGGTCCGATACT
TTCCTGATGATGATCATTTGAGCCTCAAAGATTTAGTCAAGAATGAGAAAATAGGAACAGATATCAACCA
AAACAGACTTTTCATGAAAATGGCATCTAAGTTTATGGGAAAATCAGATGAAGACAATTATACTCTGGAT
GACATGTTTGTCTCCAAAGCAGCTGAGAAAGAGCATCTTGGTAAGAAGGAAGAGAGCCAGAGGAGAAGAG
CGATTGCTGAGCACCAGAGTCTTGCTGCAAAAATGGCAAAGTGCCTTTACTGTTTTGACAGCTCTCAGTT
CCCCAAGCACCTTATTGTTGCAATCGGTGTGAAGGTTTATTTATGTTTACCCAGTTTCCAGTCTCTTACT
GAAGGACACTGCTTCATAGTCCCTCTGCAACATCATCAAGCAGCCACTGTATTAGATGAAGATGTCTGGG
AGGAGATCCAGATGTTTCGAAAATCATTGGTAAAAATGTTTGAAGATAAAGAATTGGATTGTATCTTTTT
GGAGACTAATATGGGTTTGAAGAAACACTATCATATGGTTTATGAGTGTATCCCTCTCCCAAAGGAAGTG
GGTGATATGGCTCCCATCTATTTTAAGAAAGCCATAATGGAATCTGATGAAGAATGGTCCATGAATAAGA
AGTTGATTGATTTGTCTTCAAAAGATATTAGAAAGTCTGTGCCCAGAGGTTTGCCTTACTTTGCTGTCGA
CTTTGGTCTTCAGGGAGGTTTTGCCCACATCATTGAAGATCAGTACAGATTCCCTCATTACTTTGGAAAG
GAAATCATTGGTGGTATGCTGGATCTAGAACCTAGACTATGGAGGAAGGGGATCCGAGAAAGCTTTGAGG
ACCAGAGGAAGAAATCATTGCAGTTTGCTCAGTGGTGGAAACCATATGATATCACCAAAAGTAAAAGCTC
T

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215770 representing NM_027545
Red=Cloning site Green=Tags(s)

MEAFSVRFESASSIEERKEQTRNARAEVLRQAKHNFEKEQRGEERKRLRDEDTWMLPDVHERIEQFSQEH
SEKKKKKKDKHSKKVKKEKKKKRKKQKCQKQSESTDSSASSEDEWVEAAPSQISDKEKTWKVKDKRTEEE
CDSHDIQRDEWMTIDFMSIKTVSSSSLKAEKETLRQIEREKTQVLEQSKLLERELNPYWKDGGTGLPSKT
CILPVTKAKGVEDGGLSWLRKSCQRMKEQAQKENRNFEDIVAEKYGSMEIFQSKLKEAEKIAYKKEDCGW
ERWRKPTYSDRAQCSQASGTSDLVKCKNLSEDRHLEMEPANSSNYKFSGPDTGKRSGTLQTCRRESALRK
NQDSSGNLRSKFLRPSDEDELSFHKRKNFESSSSYSPLVAQASLHCDFRKLTENSEESSASCSRSDRRQE
NRKPSDKKPLETWSYNANQHSTGGRREQLQAESMSCDPPGRGLQQDMTLTIAGPEAESTYILNVDEKNKL
GAKIIKAEMMGNMELAEQLKAQLKEANKFKETQMPAKRLGVEHEDEQEVILIQTDKSGRMWPVSSPRETL
DMKAERRKRKRVSTHEDKERVRYFPDDDHLSLKDLVKNEKIGTDINQNRLFMKMASKFMGKSDEDNYTLD
DMFVSKAAEKEHLGKKEESQRRRAIAEHQSLAAKMAKCLYCFDSSQFPKHLIVAIGVKVYLCLPSFQSLT
EGHCFIVPLQHHQAATVLDEDVWEEIQMFRKSLVKMFEDKELDCIFLETNMGLKKHYHMVYECIPLPKEV
GDMAPIYFKKAIMESDEEWSMNKKLIDLSSKDIRKSVPRGLPYFAVDFGLQGGFAHIIEDQYRFPHYFGK
EIIGGMLDLEPRLWRKGIRESFEDQRKKSLQFAQWWKPYDITKSKSS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027545
ORF Size 2661 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027545.2, NP_081821.1
RefSeq Size 3953 bp
RefSeq ORF 2664 bp
Locus ID 244672
UniProt ID Q8BG79
Cytogenetics 9 A1
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Documents
Resources
"NM_027545" in other vectors (4)
SKU Description Size Price
MC222447 Cwf19l2 (untagged) - Mouse CWF19-like 2, cell cycle control (S. pombe) (Cwf19l2), (10ug) 10 ug
$894.00
MR215770 Cwf19l2 (Myc-DDK-tagged) - Mouse CWF19-like 2, cell cycle control (S. pombe) (Cwf19l2) 10 ug
$589.00 $812.00
MR215770L3 Lenti ORF clone of Cwf19l2 (Myc-DDK-tagged) - Mouse CWF19-like 2, cell cycle control (S. pombe) (Cwf19l2) 10 ug
$1,112.00
MR215770L4 Lenti ORF clone of Cwf19l2 (mGFP-tagged) - Mouse CWF19-like 2, cell cycle control (S. pombe) (Cwf19l2) 10 ug
$1,112.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.