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Kcns3 (NM_173417) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215743
Kcns3 (tGFP-tagged) - Mouse potassium voltage-gated channel delayed-rectifier subfamily S member 3 (Kcns3) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Kcns3 or NM_173417"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kcns3
Synonyms D12Ertd137e
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215743 representing NM_173417
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGTTTGGTGAGTTTTTCCATCGCCCTGGACAAGATGAGGAACTTGTCAACTTGAACGTGGGGGGCT
TTAAGCAGTCTGTGGATCAAAGTACACTCCTGCGGTTCCCTCACACACGACTGGGAAAGCTGCTTACCTG
CCACTCTGAGGAGGCCATTCTGGAGCTGTGTGATGACTACAGCGTGGCAGATAAAGAGTACTACTTTGAT
CGGAACCCCTTCCTGTTCAGATACGTCTTGAACTTTTATTACACAGGGAAGCTGCATGTGATGGAGGAAC
TGTGTGTCTTCTCCTTCTGCCAGGAGATCGAGTACTGGGGCATCAATGAGCTCTTCATTGACTCCTGCTG
TAGCAGTCGGTACCAGGAGCGCAAGGAGGAGAGCCACGACAAGGACTGGGACCAGAAAAGCAACGATGTG
AGCACAGACTCCTCCTTTGAAGAATCGTCTCTGTTTGAGAAAGAGCTGGAGAAGTTTGATGAGCTGAGAT
TTGGTCAGCTCCGAAAGAAGATCTGGATTCGAATGGAAAATCCAGCTTACTGCCTGTCGGCCAAGCTCAT
TGCCATCTCCTCCTTGAGCGTGGTGCTGGCTTCCATAGTGGCCATGTGTGTGCACAGCATGTCGGAATTC
CAGAACGAGGATGGAGAAGTGGATGACCCTGTGCTGGAAGGTGTGGAGATTGCCTGCATTGCATGGTTTA
CTGGTGAGCTAGCCATCAGGCTGGTTGCTGCTCCATCACAAAAGAAGTTCTGGAAAAACCCTCTGAACAT
CATTGACTTTGTCTCTATCATTCCCTTCTATGCCACGTTGGCTGTGGACACCAAGGAAGAAGAGAGTGAG
GACATTGAGAATATGGGCAAGGTGGTCCAGATCCTTCGGCTCATGAGGATTTTCCGAATTCTGAAGCTTG
CCCGGCACTCTGTAGGGCTTCGGTCTCTTGGGGCCACACTGAGGCACAGTTACCATGAGGTGGGGCTACT
GCTTCTCTTCCTTTCTGTGGGCATCTCCATCTTCTCTGTGCTTATCTACTCTGTGGAGAAAGATGAACAC
AAGTCCAGTCTCACCAGCATCCCCATCTGCTGGTGGTGGGCCACTATCAGTATGACCACAGTGGGCTATG
GAGACACCCACCCAGTCACCTTAGCTGGGAAAATCATTGCAAGCACATGTATTATCTGTGGAATCTTAGT
GGTAGCCCTCCCCATTACCATCATCTTCAACAAGTTTTCCAAGTACTACCAGAAGCAGAAAGACATGGAA
GTGGACCAGTGCAGCGAGGACCCACCAGAGAAGTGCCATGAGCTACCGTACTTTAACATTAGGGACGTTT
ATGCACAGCAAGTACATGCCTTCATCACCAGTCTGTCTTCCATTGGCATCGTGGTCAGTGATCCTGACTC
CACAGATGCTTCGAGCGTTGAAGACAATGAGGATGCTTACAACACTGCATCCCTGGAGAACTGTACTGGA
AAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215743 representing NM_173417
Red=Cloning site Green=Tags(s)

MVFGEFFHRPGQDEELVNLNVGGFKQSVDQSTLLRFPHTRLGKLLTCHSEEAILELCDDYSVADKEYYFD
RNPFLFRYVLNFYYTGKLHVMEELCVFSFCQEIEYWGINELFIDSCCSSRYQERKEESHDKDWDQKSNDV
STDSSFEESSLFEKELEKFDELRFGQLRKKIWIRMENPAYCLSAKLIAISSLSVVLASIVAMCVHSMSEF
QNEDGEVDDPVLEGVEIACIAWFTGELAIRLVAAPSQKKFWKNPLNIIDFVSIIPFYATLAVDTKEEESE
DIENMGKVVQILRLMRIFRILKLARHSVGLRSLGATLRHSYHEVGLLLLFLSVGISIFSVLIYSVEKDEH
KSSLTSIPICWWWATISMTTVGYGDTHPVTLAGKIIASTCIICGILVVALPITIIFNKFSKYYQKQKDME
VDQCSEDPPEKCHELPYFNIRDVYAQQVHAFITSLSSIGIVVSDPDSTDASSVEDNEDAYNTASLENCTG
K

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173417
ORF Size 1473 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173417.3, NP_775593.1
RefSeq Size 2933 bp
RefSeq ORF 1476 bp
Locus ID 238076
UniProt ID Q8BQZ8
Cytogenetics 12 5.58 cM
Summary Potassium channel subunit that does not form functional channels by itself. Can form functional heterotetrameric channels with KCNB1; modulates the delayed rectifier voltage-gated potassium channel activation and deactivation rates of KCNB1. Heterotetrameric channel activity formed with KCNB1 show increased current amplitude with the threshold for action potential activation shifted towards more negative values in hypoxic-treated pulmonary artery smooth muscle cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_173417" in other vectors (4)
SKU Description Size Price
MC216898 Kcns3 (untagged) - Mouse potassium voltage-gated channel, delayed-rectifier, subfamily S, member 3 (Kcns3), transcript variant 1, (10ug) 10 ug
$503.00
MR215743 Kcns3 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, delayed-rectifier, subfamily S, member 3 (Kcns3), transcript variant 1 10 ug
$289.00 $457.00
MR215743L3 Lenti ORF clone of Kcns3 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, delayed-rectifier, subfamily S, member 3 (Kcns3), transcript variant 1 10 ug
$757.00
MR215743L4 Lenti ORF clone of Kcns3 (mGFP-tagged) - Mouse potassium voltage-gated channel, delayed-rectifier, subfamily S, member 3 (Kcns3), transcript variant 1 10 ug
$757.00

file-alt Citations

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