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Pcgf2 (NM_009545) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215739
Pcgf2 (tGFP-tagged) - Mouse polycomb group ring finger 2 (Pcgf2) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pcgf2
Synonyms mel-18; Mel18; Rnf110; Zfp144
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215739 representing NM_009545
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATCGGACCACACGGATTAAAATCACGGAGCTGAACCCTCACCTCATGTGTGCCCTCTGTGGGGGCT
ATTTTATTGATGCCACCACCATTGTGGAATGCTTACATTCCTTCTGCAAAACCTGCATCGTACGCTACTT
GGAGACCAACAAATACTGCCCCATGTGTGACGTCCAGGTCCATAAAACTCGACCACTGCTGAGCATCAGA
TCAGACAAAACCCTCCAGGACATTGTCTACAAGTTGGTGCCTGGGCTTTTTAAAGATGAAATGAAACGGC
GACGGGACTTCTATGCAGCATACCCCCTGACAGAGGTCCCCAACGGCTCCAACGAGGACCGAGGCGAGGT
CCTGGAACAGGAGAAGGGGGCTCTGGGTGACGACGAGATTGTCAGTCTTTCCATTGAGTTCTACGAAGGC
GTCAGGGACCGAGAGGAGAAGAAGAACCTCACGGAGAATGGAGATGGGGACAAGGAGAAGACAGGAGTGC
GATTCCTGCGGTGCCCAGCAGCCATGACCGTCATGCACCTGGCAAAGTTCCTCCGCAACAAAATGGATGT
GCCCAGCAAGTACAAGGTGGAGATCCTCTATGAAGATGAGCCCCTGAAGGAATACTACACCTTAATGGAC
ATTGCGTACATCTACCCCTGGCGAAGGAATGGGCCTCTCCCCCTCAAGTACCGTGTCCAGCCAGCCTGCA
AGCGGCTCACCCTGCCCACGGTGCCAACCCCCTCAGAAGGCACCAACACCAGCGGGGCATCAGAGTGTGA
GTCAGTCAGCGACAAGGCTCCCAGCCCTGCCACCCTTCCGGCCACCTCCTCCTCCTTGCCCAGCCCTGCG
ACCCCATCCCATGGCTCTCCCAGCTCCCACGGCCCCCCAGCTACCCATCCTACTTCCCCCACTCCACCTT
CAACTGCCGCTGGGACCACCACAGCTACCAATGGGGGCACTTCGAACTGCCTGCAAACGCCATCCTCCAC
CAGCAGGGGGCGCAAGATGACTGTTAATGGCGCTCCTTGCCCCCCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215739 representing NM_009545
Red=Cloning site Green=Tags(s)

MHRTTRIKITELNPHLMCALCGGYFIDATTIVECLHSFCKTCIVRYLETNKYCPMCDVQVHKTRPLLSIR
SDKTLQDIVYKLVPGLFKDEMKRRRDFYAAYPLTEVPNGSNEDRGEVLEQEKGALGDDEIVSLSIEFYEG
VRDREEKKNLTENGDGDKEKTGVRFLRCPAAMTVMHLAKFLRNKMDVPSKYKVEILYEDEPLKEYYTLMD
IAYIYPWRRNGPLPLKYRVQPACKRLTLPTVPTPSEGTNTSGASECESVSDKAPSPATLPATSSSLPSPA
TPSHGSPSSHGPPATHPTSPTPPSTAAGTTTATNGGTSNCLQTPSSTSRGRKMTVNGAPCPP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009545
ORF Size 1026 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009545.2, NP_033571.1
RefSeq Size 2752 bp
RefSeq ORF 1029 bp
Locus ID 22658
UniProt ID P23798
Cytogenetics 11 61.06 cM
Summary Transcriptional repressor (PubMed:8521824). Binds specifically to the DNA sequence 5'-GACTNGACT-3' (PubMed:8521824). Has tumor suppressor activity (PubMed:8521824). May play a role in control of cell proliferation and/or neural cell development (Probable). Regulates proliferation of early T progenitor cells by maintaining expression of HES1(PubMed:15728456). Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells (PubMed:8625838, PubMed:15183898). Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009545" in other vectors (5)
SKU Description Size Price
MC202903 Pcgf2 (untagged) - Mouse polycomb group ring finger 2 (Pcgf2), transcript variant 1, (10ug) 10 ug
$457.00
MG205143 Pcgf2 (tGFP-tagged) - Mouse polycomb group ring finger 2 (Pcgf2) 10 ug
$657.00
MR215739 Pcgf2 (Myc-DDK-tagged) - Mouse polycomb group ring finger 2 (Pcgf2), transcript variant 1 10 ug
$289.00 $457.00
MR215739L3 Lenti ORF clone of Pcgf2 (Myc-DDK-tagged) - Mouse polycomb group ring finger 2 (Pcgf2), transcript variant 1 10 ug
$757.00
MR215739L4 Lenti ORF clone of Pcgf2 (mGFP-tagged) - Mouse polycomb group ring finger 2 (Pcgf2), transcript variant 1 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.