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AF529169 (NM_153509) Mouse Tagged ORF Clone

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SKU
MG215719
AF529169 (tGFP-tagged) - Mouse cDNA sequence AF529169 (AF529169), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,040.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol AF529169
Synonyms DD1; Kiaa1024
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215719 representing NM_153509
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCCAATCAGGAAGCTTCCCTCTTTTTGGTGAAGATCTTAGAAGAACTGGACAGTAAACAAAATA
CTGTTTCCTATCAAGACCTGTGCAAGTCACTCTGTGCCCAGTTTGATCTGTCTCAGCTTGCCAAACTGAG
AAGTGTGCTTTTCTATACAGCATGCCTCGATCCCAACTTTCCAGCAACTTTATTCAAAGACAAGATGAAA
TGCTCTGTAAATAATCAGCAGTCAAAGAAAATCATGGTGGCAGCAGATATCGTAACAATCTTCAACCTGA
TCCAGATGAATGGGGGCACTGCCAAGGAGAAGCTGCCCATGAGTTGCCACAAGGTGCGCAAGAAGGAAGC
ATCCTTTGAGTCTTGCCGGTCCGACACCGAGGTGTGCAGCCCGACAGTTTGTGAGCCTCTGAACTGTGAG
CTGAGTGAGAGACCCTTCAGCCGAGGCTACCCCACCAGGCAGTCATCCAAATGCCGGAAGATGGACTGTA
AGGAATGCCCACAGTTTGTCCCTGCCTCTGAGCCCAACTTCCTGTTGGGTGTTAGCAAAGAGGTGAAAAA
TCGGGCAGCCTCTCTGGATAGGCTGCAAGCCCTGTCCCCCTATTCTGTGGCCAGCCCTCAGCCCTGTGAG
ATGCAGAGGACCTATTTCCCCATGAATATTGAGAACGAGCCCATGTCAGATCAGGACTCTCTGCCCATCA
GCCAAGGCATCAAAGAGACCTTCATCTCCAGTGAGGAACCGTTTGTGGTTCAGTCCTGTGTTCAGAAAAG
GAACATCTTCAAAGAAGACTTCCACAATCTCATGACTGTATCCCCCAGTTTGGTTGGTACAACTAACAAA
GCAGAAGAGGGTCATGGGGAGCCCCAGAGTCAGAAGGAGCTCCACAAGCCACCCTTCTTCAACCACAGCT
TTGAGATGCCTTACCACAACCAATACCTGAATCCGGTGTATTCCCCCATCCCTGACAAAAGGCGGGCGAA
GCATGAAAGCTTAGATGACCTTCAAGCCTCTACCTATTTTGGACCCACTCCAGTGATGGGAACACAAGAT
ACCAGGCGCTGTCCAGGGAGGTCCAGTAAACAGACTCCATGGCCCGCCAAAAGCTGGAGCCTAAACACGG
AGGAAGTGCCTGATTTCGAACGCTCCTTTTTCAATAGAAATCCCTCTGAAGAGAAGCTACGCTATCCAAA
TTCTGGCAGCCAGACTCCCAATTTCTCAGGCCCAGACAGGCATCCAGTTTACTTGGTGCCAAAGGATCAA
CAGAAGGTTCTCCCTGCTGGCTATGCAGTGAAACCAAATGGACTCAAATCGAAAGAGATCTCGTCCCCTG
TTGACCTAGAGAAGCACGAAGCAGTCAAGAAGTTTAAAGACAAAAGCATCAGCTGCACCAGTGGGCAGCA
CAGCTCAGACACCAGTAGTGTGGGTACCCAGACAGAGCAGCATGTGCTGGATCCCCCCAAATGCAAAGAT
TTGTGTACTTCGGGTCAGGCCAAGTACGGTGACAGGCATGCCATGAAGCAGTCGGACGATGACTCAGAAA
TCGTCAGCGACGACATCAGTGACATTTTCCGGTTTCTCGATGACATGAGCATCAGCGGCTCCACAGGAGT
GATACAGTCATCCTGCTACAACAGCACCGGCTCCTTGTCTCAGCTTCATAAGTCAGACTGTGATAGTTCG
CCAGAGCACCACCTCGCCAAAATCACCAATGGGGTCTCCAGCGGCAAGGGGGACAAGTGTAACCGGCCTG
AAAACGTCCATCACTCCGAGGAAGAGTTGAAGAGTAGCGTGTGCAAACTGGTTCTCAGGATTGGTGAGAT
AGAGCGGAAGCTGGAATCGCTGTCTGGGGTCCGAGAGGAAATCTCCCAGGTCCTTGGGAAACTCAATAAA
CTGGATCAGAAAATACAGCAGCCGGAGAAAGTGAATGTGCAGATTGACCTGAACTCCTTGACGAGTGAGG
CACCTTCTGATGACAGCGCCTCTCCGAGGGTCTTCCGTGCACACAGTGGCTCCCACGGACCTAAGCTGGA
GAACAGTCCTGACTGGTGCTGCTCAGATGCCAGTGGAAGTAACAGTGAAAGCCTGCGTGTCAAGGCCTTA
AAGAAAAGCCTCTTTACCAGGCCATCTTCCAGGTCCCTGACTGAGGAGAACAGTGCCACAGAGTCTAAAA
TTGCCAGCATCTCGAACTCTCCCAGAGACTGGCGAACCATCACATACACCAACCGGATGAGCCTCAACGA
GGAGGAGATAAAAGATGCAGGCCCAGCCAATAACAAAGATTGGCATCGGAAATCTAAGGAGGCAGACAGG
CAGTATGACATCCCCCCACAGCACAGATTGCCCAAGCAGCCCAAAGATGGCTTCCTGGTGGAGCAGGTGT
TCAGCCCTCATCCTTACCCCACCTCCCTCAAGGGCCACATGAAGAGCAACCCCCTGTATACAGACATGCG
GCTGACTGAACTAGCTGAGGTGAAGCGGGGCCAGCCTTCTTGGACCATAGAGGAATATGCTCGGAACTCC
GGTGACAAAGGCAAGCTGACAGCTCTGGACCTGCAGACCCAAGAATCTTTAAACCCAAACAACTTAGAGT
ACTGGATGGAAGATATTTATACTCCAGGCTATGATTCATTACTAAAACGGAAAGAAGCTGAATTCAGACG
AGCCAAGGTTTGCAAGATCGCTGCTCTTATCACTGCTGCTGCATGCACGGTCATTCTTGTCATTGTTGTG
CCAATCTGCACGATGAAGTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215719 representing NM_153509
Red=Cloning site Green=Tags(s)

MEANQEASLFLVKILEELDSKQNTVSYQDLCKSLCAQFDLSQLAKLRSVLFYTACLDPNFPATLFKDKMK
CSVNNQQSKKIMVAADIVTIFNLIQMNGGTAKEKLPMSCHKVRKKEASFESCRSDTEVCSPTVCEPLNCE
LSERPFSRGYPTRQSSKCRKMDCKECPQFVPASEPNFLLGVSKEVKNRAASLDRLQALSPYSVASPQPCE
MQRTYFPMNIENEPMSDQDSLPISQGIKETFISSEEPFVVQSCVQKRNIFKEDFHNLMTVSPSLVGTTNK
AEEGHGEPQSQKELHKPPFFNHSFEMPYHNQYLNPVYSPIPDKRRAKHESLDDLQASTYFGPTPVMGTQD
TRRCPGRSSKQTPWPAKSWSLNTEEVPDFERSFFNRNPSEEKLRYPNSGSQTPNFSGPDRHPVYLVPKDQ
QKVLPAGYAVKPNGLKSKEISSPVDLEKHEAVKKFKDKSISCTSGQHSSDTSSVGTQTEQHVLDPPKCKD
LCTSGQAKYGDRHAMKQSDDDSEIVSDDISDIFRFLDDMSISGSTGVIQSSCYNSTGSLSQLHKSDCDSS
PEHHLAKITNGVSSGKGDKCNRPENVHHSEEELKSSVCKLVLRIGEIERKLESLSGVREEISQVLGKLNK
LDQKIQQPEKVNVQIDLNSLTSEAPSDDSASPRVFRAHSGSHGPKLENSPDWCCSDASGSNSESLRVKAL
KKSLFTRPSSRSLTEENSATESKIASISNSPRDWRTITYTNRMSLNEEEIKDAGPANNKDWHRKSKEADR
QYDIPPQHRLPKQPKDGFLVEQVFSPHPYPTSLKGHMKSNPLYTDMRLTELAEVKRGQPSWTIEEYARNS
GDKGKLTALDLQTQESLNPNNLEYWMEDIYTPGYDSLLKRKEAEFRRAKVCKIAALITAAACTVILVIVV
PICTMKS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153509
ORF Size 2751 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153509.2, NP_705729.1
RefSeq Size 4250 bp
RefSeq ORF 2754 bp
Locus ID 209743
UniProt ID Q8K3V7
Cytogenetics 9 E3.1
Summary Intrinsically disordered protein which may negatively regulate mTOR signaling pathway by stabilizing the mTOR complex component DEPTOR. Negatively regulates angiogenesis. Negatively regulates cell growth (By similarity). Negatively regulates neurite outgrowth in hippocampal neurons (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_153509" in other vectors (4)
SKU Description Size Price
MC222610 AF529169 (untagged) - Mouse cDNA sequence AF529169 (AF529169), (10ug) 10 ug
$924.00
MR215719 AF529169 (Myc-DDK-tagged) - Mouse cDNA sequence AF529169 (AF529169) 10 ug
$589.00 $840.00
MR215719L3 Lenti ORF clone of AF529169 (Myc-DDK-tagged) - Mouse cDNA sequence AF529169 (AF529169) 10 ug
$1,140.00
MR215719L4 Lenti ORF clone of AF529169 (mGFP-tagged) - Mouse cDNA sequence AF529169 (AF529169) 10 ug
$1,140.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.