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Twistnb (NM_172253) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215714
Twistnb (tGFP-tagged) - Mouse TWIST neighbor (Twistnb), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Twistnb or NM_172253"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Twistnb
Synonyms 2410173G11; 2810024J17Rik; D16Wsu83e
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215714 representing NM_172253
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGGGATCTGTGGAGTCTCAGCGGTCCCAGGCGGCTTCCGAGAGGCCCGTGGCGGGTCAGGCCG
GGGTCCTGCCTTGCCTGGAGTTGCCGAGCTACGCGGCGGCGTGCGCGCTGGTGGGCAGCCGCTATTCGTG
CTTGGTGGCTGCCCCGCACCGCAGGCACATCGCCCTGTCGCCCCGCTACCTGAGCAGGAAGCGCACGGGC
ATCCGCGAGCAGCTGGACGCCGAGCTCCTGCGCTACTCCGAGAGCCTTTTAGGTGTCCCTATTGCATATG
ATAACATCAGAGTTGTGGGTGAACTAGGAGATATTTATGATGATCAAGGACACATTCATCTTAACATTGA
AGCAGATTTTGTTATTTTCTGCCCTGAGCCTGGGCAGACGTTAATGGGTACAGTTAATAAAGTATCGTCT
AGCCATATTGGCTGTCTAGTCCATGGATGTTTTAACGCCTCTATCCCTAAGCCTGAGCAGATGTCATATG
AGGAGTGGCAAACATTGGAGATACATGTAGGCGATGAACTGGAATTTGATGTATTTCGCTTAGATTCAGA
TTCTGCTGGAGTGTTCTGCATTCGAGGAAAACTGAGTACCACCAGTTTGCAACTTAAGCACTCTGCAGTT
TCTGAAGATGTAGCAGAAACTGTCGTTGAAGAGGTTGTTGAAAAAACTCCAAAGAAGAAAAAGAAGAAGA
AAGACAAAGATACAGACACATGTGGAACAGTTGATAGTGTTACAGAGGTAGCAGATGTTACAGATGTCAC
GCCACAGGAAGAAACAGACATACCATGTAGTGATAATGTGAATGACTTCTTTGAGGAAGAGCCAAAGAAG
AAGAAAAAGAAGAAGAAAAGGCACCAGGAAGATCAGGACCCCATTTTCCAAGCCAGTGACTCCAGTGGCT
ACCAGAGTGACCATAACAAGAAAAAAAAGAAAAGAAAGCACAGTGAAGAGGCTAACTTTGAGTCACCTAA
GAAGAGACAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215714 representing NM_172253
Red=Cloning site Green=Tags(s)

MAAGSVESQRSQAASERPVAGQAGVLPCLELPSYAAACALVGSRYSCLVAAPHRRHIALSPRYLSRKRTG
IREQLDAELLRYSESLLGVPIAYDNIRVVGELGDIYDDQGHIHLNIEADFVIFCPEPGQTLMGTVNKVSS
SHIGCLVHGCFNASIPKPEQMSYEEWQTLEIHVGDELEFDVFRLDSDSAGVFCIRGKLSTTSLQLKHSAV
SEDVAETVVEEVVEKTPKKKKKKKDKDTDTCGTVDSVTEVADVTDVTPQEETDIPCSDNVNDFFEEEPKK
KKKKKKRHQEDQDPIFQASDSSGYQSDHNKKKKKRKHSEEANFESPKKRQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172253
ORF Size 990 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172253.2, NP_758457.1
RefSeq Size 2324 bp
RefSeq ORF 993 bp
Locus ID 28071
UniProt ID Q78WZ7
Cytogenetics 12 A3
Summary DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through its association with RRN3/TIF-IA may be involved in recruitment of Pol I to rDNA promoters.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172253" in other vectors (2)
SKU Description Size Price
MC209807 Twistnb (untagged) - Mouse TWIST neighbor (Twistnb), (10ug) 10 ug
$330.00
MR215714 Twistnb (Myc-DDK-tagged) - Mouse TWIST neighbor (Twistnb) 10 ug
$289.00 $300.00

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