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Fzd9 (NM_010246) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215690
Fzd9 (tGFP-tagged) - Mouse frizzled homolog 9 (Drosophila) (Fzd9), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $751.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fzd9
Synonyms mfz9
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215690 representing NM_010246
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGTGCCACCGCTGCTCCGCGGGGCGCTGCTGCTGTGGCAGCTGCTGGCGACGGGCGGCGCGGCGC
TGGAGATCGGCCGCTTCGATCCGGAGCGTGGCCGTGGCCCCGCACCGTGCCAAGCGATGGAGATCCCCAT
GTGCCGGGGCATTGGCTACAACCTGACCCGCATGCCCAACCTACTGGGCCACACGTCGCAGGGCGAGGCG
GCGGCGCAGCTGGCCGAGTTCTCGCCCCTCGTGCAGTACGGCTGCCACAGCCACCTGCGCTTCTTCCTCT
GCTCGCTCTACGCCCCAATGTGCACCGACCAGGTCTCCACTCCCATCCCAGCCTGCCGGCCCATGTGCGA
GCAGGCTCGCCTGCGCTGCGCCCCCATCATGGAGCAATTCAATTTCGGCTGGCCCGACTCACTCGACTGT
GCCCGGCTCCCCACGCGCAACGACCCGCACGCACTCTGTATGGAGGCACCCGAGAACGCTACAGCAGGCC
CCACAGAACCCCACAAGGGCCTGGGCATGCTCCCTGTGGCACCTCGGCCCGCGAGGCCACCGGGAGATTC
AGCCCCAGGTCCCGGCAGCGGTGGCACCTGCGACAACCCCGAGAAGTTCCAGTACGTGGAGAAGAGTCGC
TCGTGCGCTCCGCGCTGCGGGCCAGGCGTCGAGGTGTTCTGGTCTCGGCGCGATAAGGACTTCGCGCTGG
TTTGGATGGCTGTGTGGTCCGCGTTGTGTTTCTTCTCCACGGCCTTCACCGTGTTCACCTTCCTGCTGGA
GCCTCACCGGTTCCAGTACCCAGAGCGCCCGATTATCTTCCTTTCTATGTGCTACAATGTCTACTCCTTG
GCCTTCCTGATCAGAGCGGTGGCAGGTGCACAGAGTGTGGCATGCGACCAGGAGGCAGGGGCTCTGTATG
TGATCCAGGAGGGTCTGGAAAACACAGGCTGCACCCTGGTCTTCCTGTTGCTCTATTATTTCGGGATGGC
CAGCTCACTTTGGTGGGTGGTTTTGACTCTAACCTGGTTCCTGGCTGCAGGCAAAAAATGGGGCCACGAG
GCCATCGAGGCTCACGGCAGCTACTTCCACATGGCAGCGTGGGGCCTGCCAGCACTCAAAACTATCGTGG
TCCTGACTCTGCGCAAGGTGGCTGGCGATGAACTGACTGGGCTCTGCTATGTAGCCAGCATGGACCCGGC
AGCCCTCACTGGCTTTGTGTTGGTACCCCTATCTTGCTACCTGGTACTGGGTACCAGTTTCCTCCTGACC
GGTTTTGTGGCTCTCTTCCATATCCGCAAAATCATGAAGACGGGAGGCACCAATACGGAGAAGCTGGAGA
AGCTGATGGTCAAAATCGGAGTCTTTTCCATCCTTTACACAGTGCCGGCCACCTGCGTCATTGTCTGCTA
CGTTTATGAACGCCTCAACATGGACTTCTGGCGGCTTCGGGCCACAGAGCAACCATGTACTGCTGCCACC
GTGCCTGGAGGCCGGAGAGACTGCTCGCTGCCAGGGGGCTCGGTGCCCACTGTGGCTGTCTTCATGCTCA
AAATCTTCATGTCCTTGGTGGTGGGCATCACCAGTGGAGTCTGGGTATGGAGTTCCAAGACTTTTCAGAC
TTGGCAGAGCCTGTGCTACCGAAAAATGGCAGCTGGCCGAGCCCGGGCCAAGGCCTGCCGAACCCCAGGG
GGCTATGGCCGGGGTACCCACTGCCACTACAAAGCCCCCACGGTGGTCTTGCACATGACTAAGACAGACC
CCTCTCTGGAGAACCCCACACACCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215690 representing NM_010246
Red=Cloning site Green=Tags(s)

MAVPPLLRGALLLWQLLATGGAALEIGRFDPERGRGPAPCQAMEIPMCRGIGYNLTRMPNLLGHTSQGEA
AAQLAEFSPLVQYGCHSHLRFFLCSLYAPMCTDQVSTPIPACRPMCEQARLRCAPIMEQFNFGWPDSLDC
ARLPTRNDPHALCMEAPENATAGPTEPHKGLGMLPVAPRPARPPGDSAPGPGSGGTCDNPEKFQYVEKSR
SCAPRCGPGVEVFWSRRDKDFALVWMAVWSALCFFSTAFTVFTFLLEPHRFQYPERPIIFLSMCYNVYSL
AFLIRAVAGAQSVACDQEAGALYVIQEGLENTGCTLVFLLLYYFGMASSLWWVVLTLTWFLAAGKKWGHE
AIEAHGSYFHMAAWGLPALKTIVVLTLRKVAGDELTGLCYVASMDPAALTGFVLVPLSCYLVLGTSFLLT
GFVALFHIRKIMKTGGTNTEKLEKLMVKIGVFSILYTVPATCVIVCYVYERLNMDFWRLRATEQPCTAAT
VPGGRRDCSLPGGSVPTVAVFMLKIFMSLVVGITSGVWVWSSKTFQTWQSLCYRKMAAGRARAKACRTPG
GYGRGTHCHYKAPTVVLHMTKTDPSLENPTHL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010246
ORF Size 1776 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010246.1, NP_034376.1
RefSeq Size 2110 bp
RefSeq ORF 1779 bp
Locus ID 14371
UniProt ID Q9R216
Cytogenetics 5 75.08 cM
Summary Receptor for WNT2 that is coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (By similarity). Plays a role in neuromuscular junction (NMJ) assembly by negatively regulating the clustering of acetylcholine receptors (AChR) through the beta-catenin canonical signaling pathway (PubMed:24860427). May play a role in neural progenitor cells (NPCs) viability through the beta-catenin canonical signaling pathway by negatively regulating cell cycle arrest leading to inhibition of neuron apoptotic process (By similarity). During hippocampal development, regulates neuroblast proliferation and apoptotic cell death (PubMed:15930120). Controls bone formation through non canonical Wnt signaling mediated via ISG15 (PubMed:21402791). Positively regulates bone regeneration through non canonical Wnt signaling (PubMed:24391920).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010246" in other vectors (4)
SKU Description Size Price
MC219383 Fzd9 (untagged) - Mouse frizzled homolog 9 (Drosophila) (Fzd9), (10ug) 10 ug
$607.00
MR215690 Fzd9 (Myc-DDK-tagged) - Mouse frizzled homolog 9 (Drosophila) (Fzd9) 10 ug
$289.00 $551.00
MR215690L3 Lenti ORF clone of Fzd9 (Myc-DDK-tagged) - Mouse frizzled homolog 9 (Drosophila) (Fzd9) 10 ug
$851.00
MR215690L4 Lenti ORF clone of Fzd9 (mGFP-tagged) - Mouse frizzled homolog 9 (Drosophila) (Fzd9) 10 ug
$851.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.