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Pik3c2a (NM_011083) Mouse Tagged ORF Clone

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SKU
MG215684
Pik3c2a (tGFP-tagged) - Mouse phosphatidylinositol 3-kinase C2 domain containing alpha polypeptide (Pik3c2a), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,617.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pik3c2a
Synonyms Cpk-m; PI3KC2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215684 representing NM_011083
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGGCGCGCCC

ATGGCTCAGATTTCCAACAACAGTGAATTTAAACAATGTTCATCTTCACATCCAGAACCAATAAGAACCA
AAGATGTGAACAAAGCAGAAGCGTTACAGATGGAAGCAGAAGCCTTAGCAAAACTGCAGAAGGATAGACA
AATGACTGACAGCCCAAGAGGCTTTGAGCTGTCTAGCAGCACTAGACAAAGAACACAAGGTTTTAACAAA
CAGGATTATGATCTCATGGTGTTTCCTGAGTTGGATTCCCAAAAAAGAGCAGTAGATATTGATGTAGAAA
AGCTCACCCAGGCTGAACTTGAGAAGATATTGCTGGACGACAATTTTGAAACTAGAAAACCTCCTGCATT
GCCAGTTACTCCTGTTCTGAGCCCTTCGTTCTCAACACAGCTGTATCTTAGACCTAGTGGTCAAAGAGGC
CAGTGGCCCCCTGGATTATGCGGGCCTTCCACGTACACTTTACCTTCTACTTATCCTTCAGCATACAGTA
AACAGGCCACATTCCAGAATGGCTTCAGTCCAAGGATGCCCACTTTTCCATCAACAGAGTCTGTATATTT
AAGACTTCCTGGACAGTCTCCATATTTTTCATATCCTTTGACACCTGCCACACCATTTCATCCACAAGGA
AGTTTACCAGTCTATCGGCCACTAGTCAGTCCTGACATGGCAAAACTATTTGAAAAAATAGCAAGTACCT
CAGAATTTTTAAAAAATGGGAAAGCAAGGACTGATTTGGAGATAGCAAACTCGAAAGCTTCAGTCTGCAA
TCTACAGATATCTCCAAAGTCTGAAGACATCAATAAGTTTGATTGGTTAGACTTGGATCCTCTGAGTAAG
CCTAAGGTAGACTATGTGGAGGTGTTAGAACATGAAGAAGAGAAGAAGGATCCAGTTTTGCTAGCAGAGG
ATCCTTGGGATGCTGTTCTTCTTGAAGAGAGATCGCCAAGTTGTCACCTAGAAAGAAAGGTGAATGGAAA
ATCCCTTTCTGGGGCAACTGTAACAAGAAGCCAGTCTTTAATCATTCGGACAGCTCAATTTACAAAAGCC
CAGGGCCAAGTATCTCAGAAAGACCCAAATGGGACCAGTAGTTTGCCAACTGGAAGTTCTCTTCTACAAG
AATTTGAAGTACAGAATGACGAGGTGGCAGCTTTTTGTCAATCCATTATGAAATTGAAGACCAAATTTCC
ATATACTGATCACTGCACAAATCCAGGCTATTTGTTAAGTCCAGTGACAGTGCAAAGAAACATGTGTGGG
GAGAATGCCAGTGTGAAGGTCTCCATTGAAATTGAAGGGCTTCAACTACCAGTTACTTTTACATGTGATG
TGAGTTCTACTGTAGAAATAATTATAATGCAAGCCCTTTGCTGGGTACATGATGACTTGAATCAAGTGGA
TGTTGGCAGCTACATTCTGAAAGTTTGTGGTCAAGAGGAGGTTCTACAGAATAATCATTGCCTTGGAAGT
CACGAACATATTCAAAATTGTCGAAAATGGGACACAGAGATTAAATTACAGCTCTTGACCTTGAGTGCAA
TGTGCCAGAATCTGGCTCGAACAGCAGAAGATGATGAAGCACCTGTGGATTTAAACAAATACTTGTATCA
AATAGAAAAACCTTATAAAGAAGTCATGACAAGACACCCTGTTGAAGAGCTCTTAGATTCCTATCACTAC
CAAGTAGAACTGGCTCTTCAAACTGAAAACCAGCACCGAGCTGTTGATCAAGTGATTAAAGCAGTAAGAA
AAATTTGTAGTGCTTTAGATGGGGTGGAGACCCCCTCCGTTACAGAAGCAGTGAAGAAGTTAAAGCGAGC
AGTTAACCTTCCAAGGAATAAAAGTGCTGATGTGACTTCATTATCTGGAAGTGACACAAGGAAGAACTCA
ACTAAGGGGTCACTGAATCCTGAAAATCCTGTTCAAGTAAGCATGGATCACCTAACAACAGCGATTTATG
ATCTTCTCAGGCTCCATGCAAATTCTAGTAGGTGTTCTACAGGCTGTCCCCGAGGGAGCAGGAACATCAA
GGAAGCATGGACTGCAACGGAGCAGCTCCAGTTCACTGTCTATGCCGCACACGGAATTTCCAGTAACTGG
GTATCAAATTATGAAAAATACTACTTGATATGTTCCCTGTCTCACAATGGGAAGGATCTTTTTAAGCCTA
TTCAGTCAAAGAAGGTTGGCACGTACAAGAATTTCTTCTATCTTATTAAATGGGATGAACTAATCATTTT
TCCTATCCAGATATCGCAGTTGCCATTAGAATCAGTTCTTCATCTTACTCTGTTTGGAGTTTTAAATCAG
AGCAGTGGAAGTTCCCCTGATTCTAATAAACAGAGAAAGGGGCCAGAAGCTCTGGGCAAAGTTTCTTTAA
CTCTATTTGATTTTAAACGGTTTTTAACATGTGGAACTAAACTTCTCTACCTTTGGACTTCATCACATAC
AAATTCTATTCCTGGAGCAATCCCCAAAAAAAGCTATGTCATGGAAAGAATTGTGCTACAGGTTGATTTT
CCTTCTCCTGCGTTTGACATTATTTATACATCTCCTCAAATTGATAGAAACATTATACAGCAAGACAAGT
TGGAAACACTGGAGAGTGATATAAAGGGGAAACTTCTGGATATTATTCACAGAGATTCATCATTTGGACT
TTCTAAAGAAGATAAGGTCTTTTTGTGGGAAAACCGCTATTATTGCCTAAAACATCCAAATTGTCTTCCG
AAGATATTAGCAAGTGCTCCAAACTGGAAGTGGGCTAATCTTGCCAAAACTTACTCATTGCTGCATCAGT
GGCCGCCATTGTGCCCACTAGCTGCATTGGAGCTCCTTGATGCAAAATTTGCTGATCAGGAGGTGCGATC
GCTTGCTGTGAGCTGGATGGAGGCCATTAGTGATGATGAGCTAGCAGATCTGCTCCCACAGTTCGTACAG
GCTTTGAAATATGAAATTTATTTGAATAGTTCACTAGTGCGCTTCCTTCTGTCCAGGGCATTGGGAAACA
TCCAGATAGCACACAGTTTGTATTGGCTTCTCAAGGATGCTTTGCATGATACACACTTTGGAAGCAGATA
TGAACATGTGTTGGGTGCTCTCCTCTCTGTAGGAGGAAAAGGACTCAGAGAAGAGCTTTCTAAGCAGATG
AAACTTGTACAGCTTTTAGGAGGAGTGGCAGAAAAAGTAAGGCAGGCTAGTGGATCAACAAGACAGGTTG
TCCTCCAAAAGAGTATGGAACGGGTACAGTCCTTTTTTCTGAGAAATAAATGCCGTCTTCCTCTCAAACC
AAGTCTAGTGGCAAAAGAACTAAATATTAAGTCATGTTCGTTCTTCAGTTCTAATGCTATGCCTCTGAAA
GTCACAATGGTGAATGCTGACCCTCTGGGGGAAGAAATTAATGTCATGTTTAAGGTTGGTGAAGATCTTC
GGCAAGATATGTTAGCTTTACAGATGATAAAGATTATGGATAAGATCTGGCTTAAAGAGGGACTGGATCT
GAGGATGGTGATATTCAGATGCCTGTCAACTGGCCGAGATCGAGGCATGGTGGAGCTAGTTCCTGCTTCA
GATACCCTCAGGAAAATCCAAGTGGAATATGGTGTAACAGGATCCTTTAAAGATAAACCACTTGCTGAGT
GGCTGAGGAAATACAATCCTTCTGAAGAAGAATATGAAAAGGCTTCTGAGAACTTTATCTACTCTTGTGC
TGGGTGCTGTGTAGCCACCTATGTTTTAGGCATTTGTGATCGGCACAATGACAATATAATGCTTCGAAGC
ACAGGACACATGTTCCACATTGACTTTGGAAAGTTTTTGGGCCATGCACAGATGTTTGGTAGCTTCAAAA
GGGACCGAGCTCCTTTTGTGCTTACCTCTGACATGGCGTATGTCATTAATGGAGGTGAAAAGCCCACCAT
TCGTTTCCAGTTGTTTGTGGACCTCTGCTGTCAAGCCTACAACTTGATAAGAAAGCAAACAAACCTCTTT
CTTAACCTTCTCTCACTGATGATTCCTTCAGGATTGCCAGAACTCACAAGTATTCAGGATCTGAAATATG
TTAGAGATGCACTTCAGCCCCAAACTACAGATGCTGAAGCTACTATTTTCTTTACTAGGCTGATTGAGTC
AAGTTTGGGAAGCATTGCCACAAAGTTTAATTTCTTCATTCATAACCTTGCTCAGCTACGTTTTTCTGGC
CTTCCTTCTAATGATGAGCCCATCCTTTCATTCTCACCGAAAACATACTCCTTTAGACAAGATGGCCGGA
TCAAGGAAGTCTCTGTTTTCACATATCATAAGAAATACAACCCAGATAAACACTATATTTATGTGGTTCG
AATTCTAAGAGAAGGACACCTTGAACCATCATTTGTATTCCGGACATTTGATGAATTTCAGGAACTTCAC
AATAAGCTCAGTATTATTTTTCCTCTTTGGAAATTACCTGGCTTTCCTAATAGGATGGTTCTTGGAAGAA
CACACATAAAAGATGTTGCAGCCAAGAGGAAAATTGAATTAAACAGTTATTTACAGAGTTTGATGAATGC
ATCAACAGATGTAGCAGAGTGTGATCTTGTTTGTACTTTTTTCCACCCTTTACTTCGTGATGAGAAAGCT
GAAGGAATAGCTAGGTCTGCAGGTGCAGTTCCCTTCAGCCCAACTCTGGGCCAAATAGGAGGAGCAGTGA
AGTTATCTGTTTCTTACCGAAATGGCACCCTCTTCATCATGGTGATGCACATCAAAGATCTTGTGACTGA
AGATGGGGCTGACCCAAATCCCTATGTCAAAACATACCTGCTTCCAGATACCCACAAAACGTCAAAACGT
AAAACCAAAATTTCACGTAAAACTAGGAACCCAACATTCAATGAAATGCTTGTATATAGTGGATACAGCA
AAGAAACTCTGAGGCAGAGAGAACTTCAACTGAGTGTACTCAGTGCAGAATCACTGCGGGAGAATTTCTT
CTTGGGTGGAATAACCCTGCCACTGAAAGATTTCAACTTGAGCAAAGAGACAGTTAAGTGGTATCAGCTG
ACTGCGGCAACGTATCTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215684 representing NM_011083
Red=Cloning site Green=Tags(s)

MAQISNNSEFKQCSSSHPEPIRTKDVNKAEALQMEAEALAKLQKDRQMTDSPRGFELSSSTRQRTQGFNK
QDYDLMVFPELDSQKRAVDIDVEKLTQAELEKILLDDNFETRKPPALPVTPVLSPSFSTQLYLRPSGQRG
QWPPGLCGPSTYTLPSTYPSAYSKQATFQNGFSPRMPTFPSTESVYLRLPGQSPYFSYPLTPATPFHPQG
SLPVYRPLVSPDMAKLFEKIASTSEFLKNGKARTDLEIANSKASVCNLQISPKSEDINKFDWLDLDPLSK
PKVDYVEVLEHEEEKKDPVLLAEDPWDAVLLEERSPSCHLERKVNGKSLSGATVTRSQSLIIRTAQFTKA
QGQVSQKDPNGTSSLPTGSSLLQEFEVQNDEVAAFCQSIMKLKTKFPYTDHCTNPGYLLSPVTVQRNMCG
ENASVKVSIEIEGLQLPVTFTCDVSSTVEIIIMQALCWVHDDLNQVDVGSYILKVCGQEEVLQNNHCLGS
HEHIQNCRKWDTEIKLQLLTLSAMCQNLARTAEDDEAPVDLNKYLYQIEKPYKEVMTRHPVEELLDSYHY
QVELALQTENQHRAVDQVIKAVRKICSALDGVETPSVTEAVKKLKRAVNLPRNKSADVTSLSGSDTRKNS
TKGSLNPENPVQVSMDHLTTAIYDLLRLHANSSRCSTGCPRGSRNIKEAWTATEQLQFTVYAAHGISSNW
VSNYEKYYLICSLSHNGKDLFKPIQSKKVGTYKNFFYLIKWDELIIFPIQISQLPLESVLHLTLFGVLNQ
SSGSSPDSNKQRKGPEALGKVSLTLFDFKRFLTCGTKLLYLWTSSHTNSIPGAIPKKSYVMERIVLQVDF
PSPAFDIIYTSPQIDRNIIQQDKLETLESDIKGKLLDIIHRDSSFGLSKEDKVFLWENRYYCLKHPNCLP
KILASAPNWKWANLAKTYSLLHQWPPLCPLAALELLDAKFADQEVRSLAVSWMEAISDDELADLLPQFVQ
ALKYEIYLNSSLVRFLLSRALGNIQIAHSLYWLLKDALHDTHFGSRYEHVLGALLSVGGKGLREELSKQM
KLVQLLGGVAEKVRQASGSTRQVVLQKSMERVQSFFLRNKCRLPLKPSLVAKELNIKSCSFFSSNAMPLK
VTMVNADPLGEEINVMFKVGEDLRQDMLALQMIKIMDKIWLKEGLDLRMVIFRCLSTGRDRGMVELVPAS
DTLRKIQVEYGVTGSFKDKPLAEWLRKYNPSEEEYEKASENFIYSCAGCCVATYVLGICDRHNDNIMLRS
TGHMFHIDFGKFLGHAQMFGSFKRDRAPFVLTSDMAYVINGGEKPTIRFQLFVDLCCQAYNLIRKQTNLF
LNLLSLMIPSGLPELTSIQDLKYVRDALQPQTTDAEATIFFTRLIESSLGSIATKFNFFIHNLAQLRFSG
LPSNDEPILSFSPKTYSFRQDGRIKEVSVFTYHKKYNPDKHYIYVVRILREGHLEPSFVFRTFDEFQELH
NKLSIIFPLWKLPGFPNRMVLGRTHIKDVAAKRKIELNSYLQSLMNASTDVAECDLVCTFFHPLLRDEKA
EGIARSAGAVPFSPTLGQIGGAVKLSVSYRNGTLFIMVMHIKDLVTEDGADPNPYVKTYLLPDTHKTSKR
KTKISRKTRNPTFNEMLVYSGYSKETLRQRELQLSVLSAESLRENFFLGGITLPLKDFNLSKETVKWYQL
TAATYL

TRTRPLE - GFP Tag - V
Restriction Sites AscI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011083
ORF Size 5058 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011083.2, NP_035213.2
RefSeq Size 8042 bp
RefSeq ORF 5061 bp
Locus ID 18704
UniProt ID Q61194
Cytogenetics 7 61.62 cM
Summary Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011083" in other vectors (2)
SKU Description Size Price
MC224824 Pik3c2a (untagged) - Mouse phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide (Pik3c2a), (10ug) 10 ug
$1,559.00
MR215684 Pik3c2a (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide (Pik3c2a) 10 ug
$1,417.00

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