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Lcmt2 (NM_177846) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215677
Lcmt2 (tGFP-tagged) - Mouse leucine carboxyl methyltransferase 2 (Lcmt2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $828.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lcmt2
Synonyms D330024M17; Tyw4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215677 representing NM_177846
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCCGCGAGGCCGCCAGCGCCGGGCGGGAACGGTCCAGAGCACCAACGACAGCAGCTCCCTCAGCA
AACGATCGCTTGCCGCACACGGATACGTGCGCGACCCCTTTGCGGCGCTGCTGGTCCCGGGACCCGTGCG
GCGCACGCCGCTTATCCACCGCGGCTATTACGTGCGCGCGCGCGCCGTGCGTCACTGCGTGCGCGCCTTC
CTCGAACTCACTAGCGCGCTCCCGTCCCGGACCAGAGCCCAGATCCTGTCCTTGGGCTCAGGCTCCGACT
CGCTTTATTTTCGTCTGAAAGCGGCGGGCCTCCTGGCCCGAGCTGCCGTCTGGGAGGTGGACTTCCCGGA
CGTGTCTCGGCTCAAGGCGGAGAGGATCGAGGAGACCCCGGAGCTGCGTGCGCAGACCGGTCCTTTCAAG
ATCGGGGACTCGGCGTCATCTCTGTGCTTCGAGAGCGCGGACTACCGCATCCTGGGCGCGGACCTGCGGG
AGCTCCAGCGATTAGGAGAGGCCCTGGATGGCGCCGGCCTGGACGCCACCTCTCCCACGCTGCTCCTGGC
CGAGGCGGTGCTGACCTACTTGGAGCCCTCCAGTGCCACAGCCCTCATTGCCTGGGCCGCCCAGCGTTTC
CCCGACGCCCTTTTCGTGATCTATGAGCAGATGCAGCCGGGGGATGCCTTTGGGCAGATCATGCTGCAGC
ACTTTCAGCGGTTGCATTCACCCTTGCATGGCCTGGAGCTGTTTCCCGTCGTGAAGGCCCAGCGCCAGCG
CTTCCTTCAAGCTGGCTGGACTGCCTGCAGCGCCCTGGACCTGAACGAGTTCTATCGCCGCCTTCTCTCG
GCAGAAGAGCGCCAGCGGGTTGAGACGCTTGAACCCTTTGATGAATATGAGGAGTGGCATCTGAAGTGTT
CACATTATTTCATCCTGGCAGCATCTAGGGGAGACATTCTGTCAGAAACTCCAGTGTTTGAGCCCTCAGA
GGCTTCTTTTCAGATAGATCCTGCCTCGCCTTCAGGGTTTCTTTCTGCCAGAGTAGTCACTAGTGACCAC
CAGCACTCAAGCCTGAAGCGATATGGCCACGCCTCTGCCCTCTTGAGCCCTGGCGTTATTTTCAGTGCAG
GAGGCTTTGGAGAACAAGAGGGACGACACTGCCGAGTGAGCAGGTTTCACGTGCTCTCCAGATCCTGTGA
CTCGGAATGGGAAGGCTGCCAAATAAGTACCTTGGGGACTGAAGGCCAGTGGGATGGACGCCTTTATCAC
ACCATGACAAGGCTTTCTGATACTCGGGTTCTGGTTCTTGGAGGAAGACTCTCCCCAGTAAGTCCAGCCT
CTGGGGCTCTCCAACTTGATTTATACAAAAGTAAGGATAATTGCTCTGAGGGCCAAAATGTAACAGTAAC
GAAGGCTGCCTTGGAAGAAGGTTCAGTGTTGTCATGTTGGCGGCATTCAACAACGGAAGTATACTATCAG
AATCAAAGATACTTATTTGTGTATGGTGGCCGAAGTGTGGCTGAACCTGTACTAAGTGACTGTCGTTTTC
TACATGTAGAGACAATGGCTTGGGTCAGAATCCCAGTACAAGGCGCATCACCCGAAGGTCGGCATTCCCA
TAGCGCCTGCAGTTGGCAAGGGGGAGCACTTATCGCTGGAGGTCTTGGGGCTTCTGAGGAACTGTTGAGC
TCTGTACTCTTTCTCAAACCAGTGTCCTCTGGATTCCTCTGGGAATCAATAGATATCCAGCCCTCCATTA
CCCCAAGGTACTCTCACACGGCTCATGTGTTTAATGGAAAGCTGCTTCTGGTTGGAGGGGTCTGGATTCA
TTCCTCCTCAGTTCCTGGAGTGACTGTTATCTGTTTGACTACAGGGTTGAGCTCTGAATATCAGATTGAC
ACAGCGTCTGTGCCATGGCCATTAATGCTGCACAACCACAGCAGCGCCCTCCTTCCTGAAGAGCAGCAGC
TCCTGCTTATTGGAGGTGGAGGGAACTGCTTTTCCTTTGGTACTTATTTCAACCCCCACACAGTGGCATT
AGACCTTTCTTCCTTACGTTCAGGGCAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215677 representing NM_177846
Red=Cloning site Green=Tags(s)

MGPRGRQRRAGTVQSTNDSSSLSKRSLAAHGYVRDPFAALLVPGPVRRTPLIHRGYYVRARAVRHCVRAF
LELTSALPSRTRAQILSLGSGSDSLYFRLKAAGLLARAAVWEVDFPDVSRLKAERIEETPELRAQTGPFK
IGDSASSLCFESADYRILGADLRELQRLGEALDGAGLDATSPTLLLAEAVLTYLEPSSATALIAWAAQRF
PDALFVIYEQMQPGDAFGQIMLQHFQRLHSPLHGLELFPVVKAQRQRFLQAGWTACSALDLNEFYRRLLS
AEERQRVETLEPFDEYEEWHLKCSHYFILAASRGDILSETPVFEPSEASFQIDPASPSGFLSARVVTSDH
QHSSLKRYGHASALLSPGVIFSAGGFGEQEGRHCRVSRFHVLSRSCDSEWEGCQISTLGTEGQWDGRLYH
TMTRLSDTRVLVLGGRLSPVSPASGALQLDLYKSKDNCSEGQNVTVTKAALEEGSVLSCWRHSTTEVYYQ
NQRYLFVYGGRSVAEPVLSDCRFLHVETMAWVRIPVQGASPEGRHSHSACSWQGGALIAGGLGASEELLS
SVLFLKPVSSGFLWESIDIQPSITPRYSHTAHVFNGKLLLVGGVWIHSSSVPGVTVICLTTGLSSEYQID
TASVPWPLMLHNHSSALLPEEQQLLLIGGGGNCFSFGTYFNPHTVALDLSSLRSGQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177846
ORF Size 2058 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177846.3, NP_808514.2
RefSeq Size 3407 bp
RefSeq ORF 2061 bp
Locus ID 329504
UniProt ID Q8BYR1
Cytogenetics 2 E5
Summary Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity). May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_177846" in other vectors (4)
SKU Description Size Price
MC220477 Lcmt2 (untagged) - Mouse leucine carboxyl methyltransferase 2 (Lcmt2), (10ug) 10 ug
$692.00
MR215677 Lcmt2 (Myc-DDK-tagged) - Mouse leucine carboxyl methyltransferase 2 (Lcmt2) 10 ug
$589.00 $628.00
MR215677L3 Lenti ORF clone of Lcmt2 (Myc-DDK-tagged) - Mouse leucine carboxyl methyltransferase 2 (Lcmt2) 10 ug
$928.00
MR215677L4 Lenti ORF clone of Lcmt2 (mGFP-tagged) - Mouse leucine carboxyl methyltransferase 2 (Lcmt2) 10 ug
$928.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.