Dnaja3 (NM_023646) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG215670
Dnaja3 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog subfamily A member 3 (Dnaja3) transcript variant 1, (10ug)
$489.00 $657.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dnaja3
Synonyms 1200003J13Rik; 1810053A11Rik; C81173; Tid-1; Tid1l
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023646
ORF Size 1440 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023646.4, NP_076135.3
RefSeq Size 2666 bp
RefSeq ORF 1443 bp
Locus ID 83945
UniProt ID Q99M87
Cytogenetics 16 2.46 cM
Summary Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity (By similarity). Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_023646" in other vectors (4)
SKU Description Size Price
MC216696 Dnaja3 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily A, member 3 (Dnaja3), transcript variant 1, (10ug) 10 ug
$503.00
MR215670 Dnaja3 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily A, member 3 (Dnaja3), transcript variant 1 10 ug
$289.00 $457.00
MR215670L3 Lenti ORF clone of Dnaja3 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily A, member 3 (Dnaja3), transcript variant 1 10 ug
$757.00
MR215670L4 Lenti ORF clone of Dnaja3 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily A, member 3 (Dnaja3), transcript variant 1 10 ug
$757.00

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