Dnaja3 (NM_023646) Mouse Tagged ORF Clone
- TrueORF®
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Dnaja3 |
| Synonyms | 1200003J13Rik; 1810053A11Rik; C81173; Tid-1; Tid1l |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
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| ACCN | NM_023646 |
| ORF Size | 1440 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_023646.4, NP_076135.3 |
| RefSeq Size | 2666 bp |
| RefSeq ORF | 1443 bp |
| Locus ID | 83945 |
| UniProt ID | Q99M87 |
| Cytogenetics | 16 2.46 cM |
| Summary | Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity (By similarity). Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_023646" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC216696 | Dnaja3 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily A, member 3 (Dnaja3), transcript variant 1, (10ug) | 10 ug |
$503.00
|
|
| MR215670 | Dnaja3 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily A, member 3 (Dnaja3), transcript variant 1 | 10 ug |
$289.00
$457.00
|
|
| MR215670L3 | Lenti ORF clone of Dnaja3 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily A, member 3 (Dnaja3), transcript variant 1 | 10 ug |
$757.00
|
|
| MR215670L4 | Lenti ORF clone of Dnaja3 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily A, member 3 (Dnaja3), transcript variant 1 | 10 ug |
$757.00
|
|
Plasmid Map for MG215670
Download sequence for
Cloning scheme for MG215670
Circular map for MG215670
Citations
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