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Fastkd5 (NM_198176) Mouse Tagged ORF Clone

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SKU
MG215657
Fastkd5 (tGFP-tagged) - Mouse FAST kinase domains 5 (Fastkd5) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP tGFP DDK
AAV Particle:
DDK
  $899.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fastkd5
Synonyms C78212; mKIAA1792
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215657 representing NM_198176
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGCCGCTCTCAAGCTATTAGAACCTTTAAAGTACAAAGCACCTTGTAATCCTGCTTACCGTGCAG
CCCAAAGCGTGGCCCATTGGCACATGGGAAACATCACGCCACACGGGGGACAGACGCTCCCTGAATGTAA
TAGCTCCTGCCATCTTGCCAGAAAAGTGAAGAACGTTGGTGGTACCACCCTTCCTCGGAGAACCTTCACA
GCCAGCAGTGCCCACCTGGGTTTGGAGTTCAACAAAGCTTCTACCCTTAATGCCAGCACACTGCATCCAG
ACTCATCCAGTGCTGGAGGAGGTGAAGAGGATGTAGAGGTCTTTGATTCCTTCGAAGGAACCCGAGTTTT
CTTAAAGCTAAGGCCAGAGTACCAGCTTCACAGCTATAACAGAAGTGACACTCACCAACCCATAGCTGCT
TCAGAAGTTGAACTAATTTTGCACAAAGTCACATTCTACCAAAATAAACTCCAGCCAGAAGTCATAACTA
ATTATTTTTATAAGCTCAGCTCTTTGCCAGCAGAACAGAATTCTGTTTTGCTATCCAGTAACAGCTTTGC
TTTGCTCTGCCAGCTGAGTGTGAAAAACATACAGCTCTTTAACACCTCCGATCTGATCAGTATTTTGAAA
GCCTTTGTTGACTTAAGAATTCCCCCCTCCCTGTCCATGCTAGATGTGTATGAAACCAGATTTTGCCATC
AGGTATGGGAGATGACTCTAGATCAGCTTCTCCTAGTAGCTGATCTTTGGCGGAACTTAGGCCGCAGAGT
GCCTCGTTTTTTTAAAATTTTTTTTAGTTATCTTAATTTACACTGGAGAGAGCTATCTTTGTCTCAGCTG
ATTCACTTAATTTATATTATAGGTGAAAATCGTCAAGTACCACAGGACCTGATGCAAAGACTAGAATCAC
TGATCCTTAAGTATGTAGATTCAGTCAATTTAGAGGAAGTTGGTACAATCTGTTTGGGGTTTTTTAAATC
AAGTAGTAGTCTCTCTGAATTTGTCATGCGGAAAATTGGAGATCTAGCTTGTGCTAACATGCAGCATCTG
AGTAGCCACACCTTAGTTCATATTCTTAAAATGTTCCGCTTCACTCATGTAGATCATATACATTTTATGA
AGCAGTTTGGAGAAATTGCTCCTCAGAGAATTCCTTCCTTGGGGGTTCAGGGTGTTATGCACCTCACACT
TGCCTGCTCAGCCTTACGCATCCTGGATGAAAGAGTAATGAACGCAGTAGCTGCCTCTTTGCCTCCGAGG
GTAGCACACTGTCGAAGTAAAGATGTTGCCAAAATTCTGTGGTCCTTTGGAACTCTGAATTATAAGCCAC
CCAACACAGAAGAATTTTATTCCAGTCTCATAAATGAAATCCACAGAAAGATGCCTGAATTCAACCAGTA
CCCAGAGCACCTTCTCACCTGCCTGATTGGCTTGGCATTTTCTGAATACTTTCCAGTTGAGTTCATAAAT
GTTGCTCTGAGCCCAGGATTTGTCAAGTTAGCTCAAGAGAGAAGTAAGTTTGAACTCACTAAGGAACTGT
TTACTCTTGATGGTACAGTTGCCATTGAATGTCCAGATTACAAGGGCAACCGTCTTAATTCTCACCTTCA
ACAAGAAACATCTGCAAATCTGTGGAATCTAGCAAGTAAGGATATGAGATCAAAGCCTGAATTCCTAGAA
ACTCTCTATTTACTTGAGACCATGTTGGGTGGCCCCCAGTACATTAAGCACCATATGATTTTGCCTCATA
CCCGCTCTTCTGACTTAGAGGTTCAGCTTGATGCTAACATGAAACCAATGCCCTTTAATAGTGAAGCGAC
ACCAACTGAAGATGGAGCTCAATTACGGTTTAAGCAAGTGGGAGTCAGCCTTACAGATGATTTGATGAAT
CAGTTGCTGAAGGGAAAAGCCAAGAGGTATTTCCAGGGGCAAATTGAGTTAGAGACTGGCCAGCCACCCA
TGGAGTTAAGAAAGAAGACAACTGTACCCTTGGTGAACTCTGGTTGCAATGGGACAGATAGATTGGGGGA
TGGGATGGTTGGCTTGTGCCCTCTAGCCCACATGCAGCCCCCATTAGTGAAGCTAGCTATCCAGTTCACA
AACAAGAACCAATACTGCTATGGTTCCAGGGCTCTACTTGGATTGCACAATATGAAGAGGAGGCAGCTAG
TTCAAATTGGGTACCGTGTGGTAGAATTACCCCACTGGGAATGGCTTCCACTACTAAAAAGAACTCGCTT
AGAGAAGCTAGCATACCTCCATGAGAAAGTGTTCACCTCTGCTCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215657 representing NM_198176
Red=Cloning site Green=Tags(s)

MSAALKLLEPLKYKAPCNPAYRAAQSVAHWHMGNITPHGGQTLPECNSSCHLARKVKNVGGTTLPRRTFT
ASSAHLGLEFNKASTLNASTLHPDSSSAGGGEEDVEVFDSFEGTRVFLKLRPEYQLHSYNRSDTHQPIAA
SEVELILHKVTFYQNKLQPEVITNYFYKLSSLPAEQNSVLLSSNSFALLCQLSVKNIQLFNTSDLISILK
AFVDLRIPPSLSMLDVYETRFCHQVWEMTLDQLLLVADLWRNLGRRVPRFFKIFFSYLNLHWRELSLSQL
IHLIYIIGENRQVPQDLMQRLESLILKYVDSVNLEEVGTICLGFFKSSSSLSEFVMRKIGDLACANMQHL
SSHTLVHILKMFRFTHVDHIHFMKQFGEIAPQRIPSLGVQGVMHLTLACSALRILDERVMNAVAASLPPR
VAHCRSKDVAKILWSFGTLNYKPPNTEEFYSSLINEIHRKMPEFNQYPEHLLTCLIGLAFSEYFPVEFIN
VALSPGFVKLAQERSKFELTKELFTLDGTVAIECPDYKGNRLNSHLQQETSANLWNLASKDMRSKPEFLE
TLYLLETMLGGPQYIKHHMILPHTRSSDLEVQLDANMKPMPFNSEATPTEDGAQLRFKQVGVSLTDDLMN
QLLKGKAKRYFQGQIELETGQPPMELRKKTTVPLVNSGCNGTDRLGDGMVGLCPLAHMQPPLVKLAIQFT
NKNQYCYGSRALLGLHNMKRRQLVQIGYRVVELPHWEWLPLLKRTRLEKLAYLHEKVFTSAL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198176
ORF Size 2286 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198176.3
RefSeq Size 3164 bp
RefSeq ORF 2424 bp
Locus ID 380601
UniProt ID Q7TMV3
Cytogenetics 2 F1
Summary Plays an important role in the processing of non-canonical mitochondrial mRNA precursors.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_198176" in other vectors (5)
SKU Description Size Price
MC221790 Fastkd5 (untagged) - Mouse FAST kinase domains 5 (Fastkd5), transcript variant 1, (10ug) 10 ug
$814.00
MG210525 Fastkd5 (tGFP-tagged) - Mouse FAST kinase domains 5 (Fastkd5) 10 ug
$899.00
MR215657 Fastkd5 (Myc-DDK-tagged) - Mouse FAST kinase domains 5 (Fastkd5), transcript variant 1 10 ug
$589.00 $699.00
MR215657L3 Lenti ORF clone of Fastkd5 (Myc-DDK-tagged) - Mouse FAST kinase domains 5 (Fastkd5), transcript variant 1 10 ug
$999.00
MR215657L4 Lenti ORF clone of Fastkd5 (mGFP-tagged) - Mouse FAST kinase domains 5 (Fastkd5), transcript variant 1 10 ug
$999.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.